Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof
A bio-organic fertilizer and stress-resistance technology, applied in the fields of botanical equipment and methods, organic fertilizers, rice cultivation, etc., to achieve the effects of strong environmental adaptability, increased crop yield, and strong nutritional function
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Embodiment 3
[0039] Preparation of Preliminary Example 3 Saccharomyces cerevisiae bacterial agent
[0040] Saccharomyces cerevisiae (Saccharomyces cerevisiae) was purchased from the Culture Collection and Management Center of Guangdong Institute of Microbiology, product number: GIM 2.39;
[0041] 1. Inoculate the strain of Saccharomyces cerevisiae in the culture medium (the medium is prepared according to the formula provided by the supplier) and store it in the refrigerator at 4°C after adding mineral oil to the slant seed strain of the medium. Take it out and activate it twice before use. The activated bacterial strain is transferred to 250 milliliters of eggplant bottles for cultivation at a temperature of 28-30°C for 48 hours. When the bacterial strain grows well, it is washed with sterilized physiological saline, and the eluate is the inoculum of the seed tank.
[0042] 2. Add culture medium into a 1-ton fermenter, the wort juice is generally 10-15 wavelines, and adjust the pH to 6.4....
Embodiment 5
[0047] Preparation of Preliminary Example 5 Lactobacillus plantarum inoculum
[0048] (1) Inoculate Lactobacillus plantarum (purchased from China Agricultural Microorganism Culture Collection and Management Center, No. ACCC11016) in beef juice peptone slant medium (3 grams of beef extract, 5 to 10 grams of peptone) , NaC 15 g, dissolve the above ingredients with distilled water and adjust the volume to 1000 ml, adjust the pH value to 7), add mineral oil to the slant seed strain of beef juice peptone medium and store it in a refrigerator at 4°C, take it out and activate it twice before use.
[0049] (2) Preparation of solid strains
[0050] 1 part of peptone, 0.5 parts of yeast powder, 0.2 parts of ammonium citrate, 2 parts of glucose, 0.054 parts of magnesium sulfate, 1 part of beef extract, 0.2 parts of dipotassium hydrogen phosphate, 0.5 parts of sodium acetate, 0.025 parts of manganese sulfate, 1.5 parts of agar, 93.021 parts of water, natural pH value, divided into test t...
Embodiment 8
[0065] Preliminary Example 8 Preparation of Organic Mixture Adsorbed with Composite Microorganism Liquid Preparation
[0066] (1) Preparing a compound microbial liquid preparation: mixing Lactobacillus plantarum, Lactobacillus acidophilus and water in a weight ratio of 30:50:20 to obtain a compound microbial liquid preparation;
[0067] (2) Adsorb the compound microbial liquid preparation in the organic mixture formed by zeolite powder, soybean meal, pomace and peat; wherein, the parts by weight of each component are: compound microbial liquid preparation 2, soybean meal 40, pomace 25, peat 30.
[0068] Preliminary Example 9, Preparation of Organic Mixture Adsorbed with Composite Microorganism Liquid Preparation
[0069] (1) Preparing a compound microbial liquid preparation: mixing Lactobacillus plantarum, Lactobacillus acidophilus and water in a weight ratio of 50:30:20 to obtain a compound microbial liquid preparation;
[0070] (2) Adsorb the compound microbial liquid prep...
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