Efficient composite microbial agent applied to black and odorous water body treatment and preparation method thereof

A technology of composite microbial bacterial agent and microbial bacterial agent, which is applied in the field of high-efficiency composite microbial bacterial agent and its preparation, can solve problems such as secondary pollution, achieve the effects of less dosage, accelerated growth and reproduction, and reduced eutrophication

Active Publication Date: 2018-06-01
BEIJING SOUND ENVIRONMENTAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the effect of chemical agents is remarkable, it is easy to introduce high-concentration chemical substances, resulting in secondary pollution

Method used

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preparation example Construction

[0028] The embodiment of the present invention also provides a method for preparing a high-efficiency composite microbial agent for the treatment of black and odorous water, which is used to prepare the above-mentioned composite microbial agent, comprising the following steps:

[0029] Using Bacillus subtilis, Bacillus licheniformis, Candida utilis, Rhodopseudomonas palustris, cellulase, amylase, protease, lipase and biological growth promoter as raw materials;

[0030] Bacillus subtilis, Bacillus licheniformis, Candida utilis and Rhodopseudomonas palustris in the above raw materials were respectively fermented and cultivated to obtain four kinds of fermented liquids, and then the four fermented liquids were mixed to form a mixed fermented liquid, passed After centrifuging the mixed fermentation broth to collect the bacteria, add trehalose, glycerol and β-cyclodextrin, and then freeze-dry to obtain the microbial bacterial agent, and combine the obtained microbial bacterial agen...

Embodiment 1

[0048] This embodiment provides a kind of high-efficiency composite microbial bacterial agent for black and odorous water treatment, and its preparation method is as follows:

[0049] Inoculate Bacillus subtilis, Bacillus licheniformis, Candida utilis, and Rhodopseudomonas palustris into LB medium, LB medium, YPD medium, and RCVBN medium slant medium respectively, at 35°C and 35°C, respectively. Cultivate for 20h, 20h, 40h, 100h at ℃, 28℃, 25℃ for fermentation;

[0050] The activated slant strains were inoculated in the seed medium (LB medium, LB medium, YPD medium, RCVBN medium) respectively, and cultured at 35°C, 35°C, 28°C, 25°C for 6h, 6h, 6h, 12h obtain seed liquid;

[0051] The seed solution was inoculated into the fermentation medium (LB medium, LB medium, YPD medium, RCVBN medium) according to the inoculation ratio (volume ratio) of 3%, 3%, 3%, and 10%, respectively, and was incubated at 35°C, After culturing at 35°C, 28°C, and 25°C for 20h, 20h, 40h, and 100h, four ...

Embodiment 2

[0056] This embodiment provides a kind of high-efficiency composite microbial bacterial agent for black and odorous water treatment, and its preparation method is as follows:

[0057] Inoculate Bacillus subtilis, Bacillus licheniformis, Candida utilis, and Rhodopseudomonas palustris into LB medium, LB medium, YPD medium, and RCVBN medium slant medium respectively, at 40°C and 40°C, respectively. Cultivate at ℃, 32℃, 32℃ for 40h, 40h, 60h, 150h for fermentation;

[0058] The activated slant strains were inoculated in the seed medium (LB medium, LB medium, YPD medium, RCVBN medium) respectively, and cultured at 40°C, 40°C, 32°C, 32°C for 18h, 18h, 18h, 36h obtain seed liquid;

[0059] The seed liquid was inoculated into the fermentation medium (LB medium, LB medium, YPD medium, RCVBN medium) according to the inoculation ratio (volume ratio) of 8%, 8%, 8%, and 30%, respectively, and was incubated at 37°C, After culturing at 37°C, 30°C, and 30°C for 40h, 40h, 60h, and 150h, four...

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Abstract

The invention discloses an efficient composite microbial agent applied to black and odorous water body treatment and a preparation method thereof. The composite microbial agent is mainly prepared frombacillus subtilis, bacillus licheniformis, candida utilis, rhodopseudomonas palustris, an enzyme preparation and a bio-energizer. The preparation method comprises the following steps: fermenting andculturing the four microorganisms separately; mixing fermentation liquor according to the proportion of (1 to 3) to (1 to 3) to (1 to 3) to (1 to 3); centrifugally collecting microbial inoculum; adding mycose, glycerinum and beta-cyclodextrin and performing freeze drying to obtain a microbial agent; compounding the microbial agent with the enzyme preparation and the bio-energizer to obtain the composite microbial agent. The composite microbial agent disclosed by the invention plays a role in efficiently and rapidly clarifying black and odorous water, is low in production cost, is easy to use and operate, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of sewage treatment, in particular to a high-efficiency composite microbial bacterial agent for treating black and odorous water bodies and a preparation method thereof. Background technique [0002] With the rapid economic development and the increase of urban population, most industrial wastewater and domestic sewage are directly discharged into rivers or lakes without effective treatment. With the continuous increase of organic pollutants flowing into rivers and lakes, the self-purification capacity of rivers and lakes has been exceeded, resulting in changes in water ecology, and finally resulting in seasonal or year-round black and odorous phenomena in water bodies. [0003] Black and odorous water is mainly formed by the deposition of organic sludge and microbial anaerobic fermentation. When a large amount of organic pollutants enter the water body and the dissolved oxygen in the water is exhausted by aerobic mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/125C12R1/10C12R1/72C12R1/38
CPCC02F3/34C02F3/342C02F3/347C02F2103/007C02F2303/02C12N1/16C12N1/20
Inventor 李晨刘希强张艳艳巩峰张丽云赵鹏娟赵雪莲邱国勤刘金泉
Owner BEIJING SOUND ENVIRONMENTAL ENG
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