Compound enzyme used for methane gas fermentation and capable of supplementing trace elements

A trace element, biogas fermentation technology, applied in the field of compound enzymes, can solve the problems of low utilization rate of raw materials, slow gas production rate, short gas production time, etc., and achieves the effects of fast reaction speed, increased gas production, and improved utilization rate

Active Publication Date: 2011-10-05
枣庄市杰诺生物酶有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, due to the restriction of the biogas technology by the raw material structure and the growth environment of the bacteria, the gas production speed is slow, the gas production is small, the gas production time is short, the raw material utilization rate is low, and there are many problems such as residual energy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Taking 100 grams as an example, the following formula can be taken:

[0029] Glycanase: 25g

[0030] Cellulase: 20g

[0031] Lipase: 10g

[0032] Pectinase: 7g

[0033] Protease: 8g

[0034] Amylase: 5g

[0035] Glucoamylase: 15g

[0036] Glucanase: 10g

Embodiment 2

[0038] Xylanase: 29g

[0039] Cellulase: 20g

[0040] Lipase: 8g

[0041] Pectinase: 7g

[0042] Protease: 5g

[0043] Amylase: 5g

[0044] Glucoamylase: 16g

[0045] Glucanase: 10g

[0046] Two, the percentage by weight of the trace element part is:

[0047] Zinc sulfate 10-15%, phosphate rock powder 20-30%, steelmaking slag 10-20%, calcium carbonate 12-16%, furnace ash 20-25%, activated carbon 5-10% and nonionic surfactants (such as : JFC, AEO, AE, alkanolamide, etc.) 4-8% as the main component.

[0048] Trace element part embodiment 1:

[0049] Taking 100 grams as an example, the following formula can be taken:

[0050] Zinc sulfate: 14g

[0051] Phosphate rock powder: 20g

[0052] Steelmaking slag: 17g

[0053] Calcium carbonate: 16g

[0054] Furnace ash: 20g

[0055] Activated carbon: 5g

[0056] Nonionic surfactant (AEO-9): 8g

[0057] Trace element part embodiment 2:

[0058] Taking 100 grams as an example, the following formula can be taken:

[0059] ...

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PUM

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Abstract

The invention discloses compound enzyme used for methane gas fermentation and capable of supplementing trace elements, which is used for quickly decomposing raw materials in methane gas fermentation to supplement the trace elements so as to quicken gas production and increase gas yield. The compound enzyme comprises an enzyme preparation part and a trace element part, wherein the enzyme preparation part is prepared by compounding the following solid monomeric enzyme raw materials in percentage by weight: 20 to 30 percent of 20,000u/g xylanase, 20 to 30 percent of 20,000u/g cellulose, 5 to 10 percent of 1,500u/g lipase, 5 to 10 percent of 10,000u/g pectinase, 5 to 10 percent of 30,000u/g saccharifying enzyme and 5 to 10 percent of 10,000u/g glucanase; the trace element part is prepared from the following raw materials in percentage by weight: 10 to 15 percent of zinc sulfate, 20 to 30 percent of powdered rock phosphate, 10 to 20 percent of steelmaking slag, 12 to 16 percent of calcium carbonate, 20 to 25 percent of stove ash, 5 to 10 percent of active carbon and 4 to 8 percent of nonionic surfactant; and a weight ratio of the enzyme preparation part to the trace element part is 1:(1-2). The compound enzyme can produce gas quickly, increase gas yield, prolong fermentation time, reduce residual energy and improve utilization rate of the raw materials.

Description

technical field [0001] The invention belongs to biological product protein engineering and enzyme engineering, and is used for rapid degradation of raw materials in biogas fermentation to replenish trace elements, to promote rapid gas production and increase gas production, and in particular to a compound enzyme used for biogas fermentation and capable of supplementing trace elements. Background technique [0002] With the long-term use of coal, oil, and natural gas, these non-renewable fossil energy sources are facing the danger of depletion. At the same time, there are a large number of crop straws, roots, stems and other wastes in my country's agricultural production every year. And animal husbandry produces a large amount of animal manure, as well as a large amount of organic waste in human production and life. [0003] Biogas technology, as a technology to produce flammable gases such as methane from livestock manure, crop stalks, food processing waste and wastewater, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50C12N9/46C12N9/42C12N9/34C12N9/26C12N9/20C12P5/02C02F11/04
CPCY02E50/343Y02E50/30
Inventor 许向阳宋在伟刘长青
Owner 枣庄市杰诺生物酶有限公司
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