Method for enriching vibrio phage and biologically preventing host bacteria

Abstract

The invention relates to a method for enriching vibrio phage and biologically preventing host bacteria. Due to splitting specificity of the phage, the phage of the specific host strains in the specific environment and a certain state cannot be obtained easily. According to the facts that the vibrio strains and the phage of culture zones and sea areas coexist with the environment ecologically and evolutionally, a long-term culture pool of the host bacteria and the phage is built in a specific area, lytic phage of specific pathogenic bacteria hosts in the area can be continuously enriched and separated, the lytic phage can be placed in the area after augmentation, proliferation of pathogenic bacteria of a cultivation water body can be controlled in a short time, concentration of the pathogenic bacteria of the water body can be restrained in a long term, and the effect of ecological defense is achieved. The method utilizes the phage in the cultivation sea water body to prevent diseases caused by the pathogenic bacteria and has the advantages of being efficient, ecological, safe, safe and lasting.

Description

technical field

[0001] The invention relates to a method for enriching Vibrio phages and biologically controlling host bacteria. The invention belongs to microbial technology for disease prevention and control in seawater aquaculture, and is suitable for marine economic animal breeding sea areas and farms. Background technique

[0002] As a common opportunistic pathogen in aquaculture, Vibrio causes great harm every year. Opportunistic pathogens are also known as opportunistic pathogens, and bacteria that can cause disease under certain conditions are called opportunistic pathogens. It can cause disease when the accumulation site of conditional pathogenic bacteria in the body changes, the body's resistance decreases, or the flora becomes imbalanced. The deterioration of the water quality in the water body, the increase of the concentration of conditioned pathogenic bacteria in the water body, and the decrease of the immune function of the farmed animals can all lead to dise...

Examples

Embodiment 1

[0031] Shrimp farming pond

[0032] In Zhanjiang Tengfei Industrial Co., Ltd., a mulch-type high-density prawn farm with a winter shed is built on 430 mu in the Anli of Donghai Island. In 2009 and 2010, the winter shed shrimp farming was completed. Construction 30m in 2009 2 , Four small cement pools with a depth of 1.5m, located at the four corners, add fresh filtered seawater (source water for aquaculture), and keep the water level not lower than 1m. Set up multi-layer filtration in the drain, and then hang the bag, which contains chlorine-containing disinfectant, and discharge it after disinfection for 1-2 days;

[0033] Four hanging oysters grown in the same sea area are placed in each pond, with about 10 oysters in each hanging, soaked in fresh seawater for 48 hours before putting in. Control phytoplankton algae by changing water, amino acid fertilizer, a small amount of fish meal and soybean meal to no more than 1-5×10 3 / mL, zooplankton not higher than 2×10 2 / mL,...

Embodiment 2

[0039] Breeding Dongfeng snails in mulched shrimp ponds

[0040] Reconstruction of 150 acres of shrimp ponds with 25 first-class water lifts in the Anli of Donghai Island is used as a place for cultivating Dongfeng snail ponds in plastic film shrimp ponds. Renovate the size of the pond, lay plastic film on the wall and bottom of the pond; renovate the water supply and drainage system, and add oxygenation facilities to make it a Dongfeng snail breeding system. Lay 5cm to 10cm of sand in the plastic film shrimp pond. Do not use too fine sand or too coarse sand. After screening with 10-mesh or 30-mesh sieve silk, take the sand of the middle size and lay it evenly on the bottom of the pool. The water depth is 60cm to 100cm. Equipped with 1 to 2 water wheel aerators with a power of 1.5kW;

[0041] 30m under construction in 2010 2 , 2 small cement pools with a depth of 1.5m, located near the drain, add fresh filtered seawater (source water for aquaculture), and keep the water le...

technical field

[0001] The invention relates to a method for enriching Vibrio phages and biologically controlling host bacteria. The invention belongs to microbial technology for disease prevention and control in seawater aquaculture, and is suitable for marine economic animal breeding sea areas and farms. Background technique

[0002] As a common opportunistic pathogen in aquaculture, Vibrio causes great harm every year. Opportunistic pathogens are also known as opportunistic pathogens, and bacteria that can cause disease under certain conditions are called opportunistic pathogens. It can cause disease when the accumulation site of conditional pathogenic bacteria in the body changes, the body's resistance decreases, or the flora becomes imbalanced. The deterioration of the water quality in the water body, the increase of the concentration of conditioned pathogenic bacteria in the water body, and the decrease of the immune function of the farmed animals can all lead to dise...

Examples

Embodiment 1

[0031] Shrimp farming pond

[0032] In Zhanjiang Tengfei Industrial Co., Ltd., a mulch-type high-density prawn farm with a winter shed is built on 430 mu in the Anli of Donghai Island. In 2009 and 2010, the winter shed shrimp farming was completed. Construction 30m in 2009 2 , Four small cement pools with a depth of 1.5m, located at the four corners, add fresh filtered seawater (source water for aquaculture), and keep the water level not lower than 1m. Set up multi-layer filtration in the drain, and then hang the bag, which contains chlorine-containing disinfectant, and discharge it after disinfection for 1-2 days;

[0033] Four hanging oysters grown in the same sea area are placed in each pond, with about 10 oysters in each hanging, soaked in fresh seawater for 48 hours before putting in. Control phytoplankton algae by changing water, amino acid fertilizer, a small amount of fish meal and soybean meal to no more than 1-5×10 3 / mL, zooplankton not higher than 2×10 2 / mL,...

Embodiment 2

[0039] Breeding Dongfeng snails in mulched shrimp ponds

[0040] Reconstruction of 150 acres of shrimp ponds with 25 first-class water lifts in the Anli of Donghai Island is used as a place for cultivating Dongfeng snail ponds in plastic film shrimp ponds. Renovate the size of the pond, lay plastic film on the wall and bottom of the pond; renovate the water supply and drainage system, and add oxygenation facilities to make it a Dongfeng snail breeding system. Lay 5cm to 10cm of sand in the plastic film shrimp pond. Do not use too fine sand or too coarse sand. After screening with 10-mesh or 30-mesh sieve silk, take the sand of the middle size and lay it evenly on the bottom of the pool. The water depth is 60cm to 100cm. Equipped with 1 to 2 water wheel aerators with a power of 1.5kW;

[0041] 30m under construction in 2010 2 , 2 small cement pools with a depth of 1.5m, located near the drain, add fresh filtered seawater (source water for aquaculture), and keep the water le...