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Compound probiotic fermentation product for preventing and treating aquatic vibriosis

A technology of compounding probiotics and fermented products, applied in the field of aquatic vibrio disease prevention and control, can solve problems such as the abuse of antibiotics and the impact on ecology

Inactive Publication Date: 2020-10-13
SICHUAN CHENGKANG ANIMAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of antibiotic abuse and ecological impact caused by the current use of antibiotics, the inventor has developed a product of compound probiotics fermented traditional Chinese medicine for the prevention and treatment of aquatic vibriosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, a compound probiotic fermentation product for preventing and treating aquatic vibriosis, the fermentation formula of the fermentation product comprises the following components by mass fraction: Scutellaria baicalensis 10%, eucalyptus leaves 10%, Dendrobium officinale 7%, Bacillus subtilis 3% of bacterial liquid, 3% of Candida utilis bacterial liquid, 17% of corn flour, 20% of soybean meal, 5% of wheat bran, 5% of brown sugar, and 20% of water; the fermentation condition of the fermentation product is: at the initial pH =6. Fermentation for 12 days under the conditions of temperature 25° C. and rotation speed 100 r / min.

[0021] The preparation method of described Bacillus subtilis bacterium liquid is:

[0022] ①Mix 10g of peptone, 3g of beef extract, 10ml of glucose, 5g of sodium chloride, 10ml of 3.08% magnesium sulfate, and 1L of water, and then sterilize under high pressure steam at 0.15MPa and 121 degrees Celsius for 20 minutes to obtain a liquid cult...

Embodiment 2

[0027] Embodiment 2, a compound probiotic fermentation product for preventing and treating aquatic vibriosis, the fermentation formula of the fermentation product comprises the following components by mass fraction: Scutellaria baicalensis 12%, eucalyptus leaves 8%, Dendrobium officinale 6%, Bacillus subtilis 4% of bacterial liquid, 4% of Candida utilis bacterial liquid, 16% of corn flour, 20% of soybean meal, 5% of wheat bran, 5% of brown sugar, and 20% of water; the fermentation condition of the fermentation product is: at the initial pH =5.5, fermented for 10 days under the conditions of temperature 28° C. and rotation speed 100 r / min.

[0028] The preparation method of described Bacillus subtilis bacterium liquid is:

[0029] ①Mix 10g of peptone, 3g of beef extract, 10ml of glucose, 5g of sodium chloride, 10ml of 3.08% magnesium sulfate, and 1L of water, and then sterilize under high pressure steam at 0.15MPa and 121 degrees Celsius for 20 minutes to obtain a liquid cultur...

Embodiment 3

[0034] Embodiment 3, a compound probiotic fermentation product for preventing and treating aquatic vibriosis, the fermentation formula of the fermentation product contains the following components in mass fraction: Scutellaria baicalensis 12%, eucalyptus leaves 10%, Dendrobium officinale 7%, subtilis Bacillus bacterium liquid 4%, Candida utilis liquid 4%, corn flour 20%, soybean meal 17%, wheat bran 5%, brown sugar 5%, water 16%; The fermentation condition of described fermentation product is: Fermentation was carried out for 12 days under the conditions of initial pH=5, temperature 28° C., and rotation speed 100 r / min.

[0035] The preparation method of described Bacillus subtilis bacterium liquid is:

[0036] ①Mix 10g of peptone, 3g of beef extract, 10ml of glucose, 5g of sodium chloride, 10ml of 3.08% magnesium sulfate, and 1L of water, and sterilize at 0.15MPa and 121 degrees Celsius for 20 minutes to obtain a liquid culture medium. ;

[0037] ②Inoculate the screened and...

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PUM

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Abstract

The invention discloses a compound probiotic fermentation product for preventing and treating aquatic vibriosis, and belongs to the field of prevention and treatment of aquatic vibriosis. The fermentation formula of the fermentation product comprises the following components in percentage by weight: 6-12% of scutellaria baicalensis, 3-10% of eucalyptus leaves, 5-7% of dendrobium officinale, 2-4% of bacillus subtilis liquid, 2-4% of candida utilis liquid, 15-20% of corn flour, 15-20% of soybean meal, 5-8% of wheat bran, 5-8% of brown sugar and 15-20% of water, wherein the fermentation conditions of the fermentation product are as follows: fermentation is carried out for 8-12 days, where the initial pH value is 5-6, the temperature is 25-28 DEG C, and the rotating speed is 50-100 r / min. Thefermentation product provided by the invention has strong killing and inhibiting effects on vibrio alginolyticus, vibrio anguillarum and vibrio parahaemolyticus of vibrio.

Description

technical field [0001] The invention relates to the field of prevention and treatment of aquatic vibriosis, in particular to a compound probiotic fermentation product for preventing and treating aquatic vibriosis. Background technique [0002] Vibriosis is a common disease in seawater aquatic products. Fish and shrimp seedlings are easily harmed by vibriosis during breeding. The pathogen of vibriosis is mainly bacteria of the genus Vibrio. The main symptoms are: sick fish, shrimp and body surface ulcers It is the main feature. In severe cases, the snout end of the head, body surface, bleeding and rot, and sometimes the internal organs have obvious lesions. The disease occurs every month, and July to August is a high incidence period, and the mortality rate can reach more than 80%. At present, the commonly used treatment method is to mix antibacterial drugs such as norfloxacin, ciprofloxacin, chloramphenicol, gentamicin, etc. into the bait, and the dosage is 1% to 3% of the ...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61P31/04C12N1/20C12N1/16C12R1/125C12R1/72A61K35/742
CPCA61K35/742A61K36/064A61K36/539A61K36/61A61K36/8984A61K2236/19A61P31/04C12N1/16C12N1/20A61K2300/00
Inventor 胡进成程超杨军黄建国
Owner SICHUAN CHENGKANG ANIMAL PHARMA
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