Gene chip of aquatic product cultivation pathogenic bacterium

A technology of gene chip and pathogenic bacteria, which is applied in the field of gene chip of pathogenic bacteria in aquatic products, to achieve the effect of quick and specific bacteria and small size
CN101691608AInactive Publication Date: 2010-04-07NINGBO UNIV +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NINGBO UNIV
Publication Date
2010-04-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a gene chip of aquatic product cultivation pathogenic bacterium, comprising a solid phase carrier which is modified chemically, a detection probe and a quality control probe are distributed on the solid phase carrier in a dot matrix way; the detection probe comprises specificity 16S rDNA sequences and / or gyrB gene sequences of vibrio, comma bacillus, vibrio harveyi, vibrio alginolyicus, vibrio anguillarum, vibrio parahemolyticus, nocardia, nacardia seriolea, aeromonas, hydrophilic aeromonas, streptococcus and dolphin streptococcus, which are to be detected, the quality control probe includes PCR positive, chip fixed positive control, chip hybridizing negative control, chip hybridizing positive control and chip hybridizing blank control; the gene chip has the advantages of small volume and high flux, can detect known and unknown germs of the vibrio, the nocardia, the aeromonas and the streptococcus, and can detect specific germs with multiple kinds, and the simpleness and rapidness and specificity of the germs can be detected, and automatic detection can be carried out after detection software is additionally arranged.
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Description

technical field

[0001] The invention relates to a gene chip, in particular to a gene chip of aquatic product cultivation pathogenic bacteria. Background technique

[0002] Due to the gradual scarcity of natural resources and the increasing demand of people, most aquatic products are currently artificially cultured. Due to the high density of aquatic products in the breeding process, the eutrophication of water bodies and the poor fluidity of water bodies, various pathogenic bacteria reproduce and the increase in infectivity have become an important problem in aquatic product farming. At present, there is a lack of detection methods for pathogenic bacteria in aquatic product farming. Farmers have to adopt the method of preventing and killing all kinds of pathogenic bacteria based on experience. Sprinkling medicine can not prescribe the right medicine, which not only causes a waste of medicine, but also fails to reach the proper dosage of killing pathogenic bacteria.

[0003] ...

Claims

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