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Gene chip of aquatic product cultivation pathogenic bacterium

A technology of gene chip and pathogenic bacteria, which is applied in the field of gene chip of pathogenic bacteria in aquatic products, to achieve the effect of quick and specific bacteria and small size

Inactive Publication Date: 2010-04-07
NINGBO UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on gene chips for high-throughput, miniaturized and automated detection of pathogenic bacteria in aquatic products

Method used

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  • Gene chip of aquatic product cultivation pathogenic bacterium
  • Gene chip of aquatic product cultivation pathogenic bacterium
  • Gene chip of aquatic product cultivation pathogenic bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] Below in conjunction with embodiment the present invention is described in further detail.

[0014] 1. Selection of solid phase carrier (substrate)

[0015] Solid phase supports include nylon membrane, cellulose membrane, glass slide, metal sheet, silicon wafer, ceramic sheet, thin film made of various organic polymers, etc. We choose glass slide with a size of 75.5mm×25.2mm×1.0mm as the substrate According to the measurement results of large batches of glass slides and the requirements of the spotting instrument for spotting substrates, the error of length and width is required to be less than or equal to ±0.2mm, and the error of thickness is less than or equal to ±0.05mm. At the same time, the appearance is required to be free from scratches and defects.

[0016] 2. Chemical modification of substrate surface

[0017] The surface of the slide must be chemically modified before the DNA can be firmly fixed. The existing chemical modifications on the surface of the slid...

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Abstract

The invention discloses a gene chip of aquatic product cultivation pathogenic bacterium, comprising a solid phase carrier which is modified chemically, a detection probe and a quality control probe are distributed on the solid phase carrier in a dot matrix way; the detection probe comprises specificity 16S rDNA sequences and / or gyrB gene sequences of vibrio, comma bacillus, vibrio harveyi, vibrio alginolyicus, vibrio anguillarum, vibrio parahemolyticus, nocardia, nacardia seriolea, aeromonas, hydrophilic aeromonas, streptococcus and dolphin streptococcus, which are to be detected, the quality control probe includes PCR positive, chip fixed positive control, chip hybridizing negative control, chip hybridizing positive control and chip hybridizing blank control; the gene chip has the advantages of small volume and high flux, can detect known and unknown germs of the vibrio, the nocardia, the aeromonas and the streptococcus, and can detect specific germs with multiple kinds, and the simpleness and rapidness and specificity of the germs can be detected, and automatic detection can be carried out after detection software is additionally arranged.

Description

technical field [0001] The invention relates to a gene chip, in particular to a gene chip of aquatic product cultivation pathogenic bacteria. Background technique [0002] Due to the gradual scarcity of natural resources and the increasing demand of people, most aquatic products are currently artificially cultured. Due to the high density of aquatic products in the breeding process, the eutrophication of water bodies and the poor fluidity of water bodies, various pathogenic bacteria reproduce and the increase in infectivity have become an important problem in aquatic product farming. At present, there is a lack of detection methods for pathogenic bacteria in aquatic product farming. Farmers have to adopt the method of preventing and killing all kinds of pathogenic bacteria based on experience. Sprinkling medicine can not prescribe the right medicine, which not only causes a waste of medicine, but also fails to reach the proper dosage of killing pathogenic bacteria. [0003]...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C40B40/06C12R1/63C12R1/46C12R1/20C12R1/37C12R1/365C12R1/01
CPCY02A50/30
Inventor 陈炯李明云史雨红李长红张亮
Owner NINGBO UNIV
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