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Microbial preparation for aquaculture water environment ammonia-nitrogen degradation and application thereof

A microbial preparation, aquaculture water technology, applied in the field of microbial preparations for ammonia nitrogen degradation in aquaculture water environment, can solve problems such as limited emergency degradation effect

Active Publication Date: 2018-11-30
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its consideration of the degradation and maintenance of ammonia nitrogen and nitrite in the water body, the emergency degradation effect on the sudden increase of ammonia nitrogen in the aquaculture water environment caused by high residual bait or other stress conditions is relatively limited. A water environment improver that can quickly alleviate the sudden increase of ammonia nitrogen is urgently needed by practitioners in this field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] In the present invention, the preparation method of Rhodopseudomonas palustris PSB20 bacterial agent preferably comprises the following steps:

[0024] (1) Inoculate the basal medium with Rhodopseudomonas palustris strain PSB20, and culture it under light at 2500-3500Lx for 72-144 hours at 25-35°C to obtain the seed liquid;

[0025] (2) inoculate the seed liquid into the propagation medium according to the inoculum amount of 5%-20% of the volume concentration, and cultivate under light at 30-35° C. for 3-5 days at 2500-3500 Lx to obtain the bacterial liquid;

[0026] (3) adding a flocculant to the bacterial liquid, and removing the supernatant after flocculation to obtain a thick bacterial paste;

[0027] (4) Adding a protective agent to the thick bacterial paste to obtain the Rhodopseudomonas palustris PSB20 microbial agent.

[0028] In the present invention, the PSB20 bacterial strain of Rhodopseudomonas palustris is first inoculated into the basic medium for light c...

Embodiment 1

[0038] (1) Cultivation of bacterial strain PSB20:

[0039] Take bacterial strain PSB20 and inoculate it into basal medium (sodium acetate 3.3g, potassium dihydrogen phosphate 0.6g, ammonium chloride 1.0g, magnesium sulfate 0.3g, calcium chloride 0.05g, sodium chloride 1.0g, yeast extract 1.0 g, manganese sulfate 0.023g, ferrous sulfate 0.005g, water 1000mL, pH 7.5~8.5), 27°C, 2500Lx light culture for 72h, when the OD of the culture solution 660 When it reached 1.5, the seed liquid was obtained.

[0040] Inoculate the seed liquid to the transparent plastic agricultural film (containing propagation medium: 1.0 g of ammonium chloride, 3.5 g of sodium acetate, 2.0 g of magnesium chloride, 0.1 g of calcium chloride, potassium dihydrogen phosphate, etc. 0.6g, 0.4g of dipotassium hydrogen phosphate, 0.1g of yeast extract, 1000mL of water, pH 7.2), 30~35°C (when the temperature is too high, use water spray to cool down), and use sunlight to cultivate for 3 days (cloudy and At night,...

Embodiment 2

[0044] Determination of the components of the product prepared in Example 1: After gradient dilution of the product prepared in Example 1 with physiological saline, each dilution was coated by the double-layer plate method and incubated at 35°C. The technical results showed that the product in Example 1 1 The number of viable PSB20 bacteria in the prepared product was 5.82×10 9 CFU / mL.

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PUM

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Abstract

The invention provides a microbial preparation for aquaculture water environment ammonia-nitrogen degradation and application thereof, and belongs to the technical field of aquaculture and water quality improvement. The microbial preparation comprises, by mass, 26-50% of rhodopseudomonas palustris PSB20 microbial inoculum and 50-74% of carrier. The rhodopseudomonas palustris PSB20 microbial inoculum is obtained by rhodopseudomonas palustris PSB20 bacterial strain culturing. The preservation number of the rhodopseudomonas palustris PSB20 bacterial strain is CGMCC No. 9632. According to the microbial preparation, ammonia nitrogen and organic matter content in the aquaculture water environment can be obviously reduced, the vibrio total in the aquaculture water environment can be obviously reduced, and efficient aquaculture water quality improvement can be achieved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture water quality improvement, and in particular relates to a microbial preparation for degrading ammonia nitrogen in the aquaculture water environment and an application thereof. Background technique [0002] With the development of the aquaculture industry, ponds are aging year by year, and the water quality and bottom quality of aquaculture are seriously polluted, resulting in frequent occurrence of aquaculture diseases. Water quality improvement has become one of the main measures for healthy aquaculture. In recent years, the research on water quality improvers is also in continuous development. Traditional water quality improvers use physical and chemical principles to improve water quality by absorbing or increasing dissolved oxygen in water. The efficiency is low, and they cannot meet the requirements of water purification in the current high-density farming mode. [0003] The Chinese inve...

Claims

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Application Information

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IPC IPC(8): C12N11/14C02F3/34C12R1/01
CPCC02F3/34C12N11/14
Inventor 唐旭黄仕新徐长安万婧倞王青华刘源森孙晓晖林凌
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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