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A kind of microbial preparation and application thereof for degradation of ammonia nitrogen in aquaculture water environment

A technology of microbial preparations and aquaculture water, which is applied in the field of microbial preparations for the degradation of ammonia nitrogen in the aquaculture water environment, and can solve problems such as limited emergency degradation effects

Active Publication Date: 2020-09-15
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its consideration of the degradation and maintenance of ammonia nitrogen and nitrite in the water body, the emergency degradation effect on the sudden increase of ammonia nitrogen in the aquaculture water environment caused by high residual bait or other stress conditions is relatively limited. A water environment improver that can quickly alleviate the sudden increase of ammonia nitrogen is urgently needed by practitioners in this field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] In the present invention, the preparation method of the Rhodopseudomonas palustris PSB20 inoculum preferably includes the following steps:

[0024] (1) Inoculate the PSB20 strain of Rhodopseudomonas palustris into the basic medium, cultivate at 25~35℃, 2500~3500Lx under light for 72~144h to obtain seed liquid;

[0025] (2) Inoculate the seed solution to a propagation medium at an inoculum of 5%-20% of the volume concentration, and cultivate at 30-35°C under 2500-3500Lx under light for 3-5 days to obtain a bacterial solution;

[0026] (3) Add a flocculant to the bacterial liquid, and remove the supernatant after flocculation to obtain a thick bacterial paste;

[0027] (4) Add a protective agent to the thick bacterial paste to obtain the Rhodopseudomonas palustris PSB20 bacterial preparation.

[0028] In the present invention, the Rhodopseudomonas palustris PSB20 strain is inoculated into a basic medium for light cultivation to obtain seed liquid. In the present invention, the bas...

Embodiment 1

[0038] (1) Cultivation of strain PSB20:

[0039] Take the strain PSB20 and inoculate it into the basic medium (3.3g sodium acetate, 0.6g potassium dihydrogen phosphate, 1.0g ammonium chloride, 0.3g magnesium sulfate, 0.05g calcium chloride, 1.0g sodium chloride, and yeast extract 1.0 g, manganese sulfate 0.023g, ferrous sulfate 0.005g, water 1000mL, pH 7.5~8.5), incubate at 27℃, 2500Lx under light for 72h, when the OD of the culture medium 660 When it reaches 1.5, the seed liquid is obtained.

[0040] The seed solution was inoculated into a transparent plastic agricultural film in an inoculum of 10% by volume (contains propagation medium: 1.0g ammonium chloride, 3.5g sodium acetate, 2.0g magnesium chloride, 0.1g calcium chloride, potassium dihydrogen phosphate 0.6g, dipotassium hydrogen phosphate 0.4g, yeast extract 0.1g, water 1000mL, pH 7.2), 30~35℃ (when the temperature is too high, spray water to cool down), and use sunlight to cultivate for 3 days (cloudy and At night, 60W / 25...

Embodiment 2

[0044] The components of the product prepared in Example 1 were determined: the product prepared in Example 1 was diluted with physiological saline, and the dilutions were coated by the double-layer plate method and incubated at 35°C. The technical results show the examples 1 The number of viable PSB20 cells in the prepared product is 5.82×10 9 CFU / mL.

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Abstract

The invention provides a microbial preparation for aquaculture water environment ammonia-nitrogen degradation and application thereof, and belongs to the technical field of aquaculture and water quality improvement. The microbial preparation comprises, by mass, 26-50% of rhodopseudomonas palustris PSB20 microbial inoculum and 50-74% of carrier. The rhodopseudomonas palustris PSB20 microbial inoculum is obtained by rhodopseudomonas palustris PSB20 bacterial strain culturing. The preservation number of the rhodopseudomonas palustris PSB20 bacterial strain is CGMCC No. 9632. According to the microbial preparation, ammonia nitrogen and organic matter content in the aquaculture water environment can be obviously reduced, the vibrio total in the aquaculture water environment can be obviously reduced, and efficient aquaculture water quality improvement can be achieved.

Description

Technical field [0001] The invention belongs to the technical field of aquaculture water quality improvement, and specifically relates to a microbial preparation for the degradation of ammonia nitrogen in aquaculture water environment and its application. Background technique [0002] With the development of the aquaculture industry, ponds are aging year by year, and the quality of aquaculture water and bottom quality are seriously polluted, resulting in frequent occurrence of aquaculture diseases, and water quality improvement has become one of the main measures for healthy aquaculture. In recent years, the research on water quality improvers is also under continuous development. Traditional water quality improvers use the principles of physics and chemistry to improve water quality by adsorbing or increasing dissolved oxygen in the water body, with low efficiency and unable to meet the requirements of water purification in the current high-density aquaculture mode. [0003] The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C02F3/34C12R1/01
CPCC02F3/34C12N11/14
Inventor 唐旭黄仕新徐长安万婧倞王青华刘源森孙晓晖林凌
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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