Bacillus licheniformis and feed additive comprising same

A technology of Bacillus licheniformis and feed additives, which is applied in animal feed, animal feed, bacteria, etc., and can solve problems such as drug-resistant strains, aquatic animal residues, and diseases caused by deterioration of the breeding environment

Active Publication Date: 2016-03-02
青岛玛斯特生物技术有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the intensification and high-density scale of aquaculture is expanding day by day, the deterioration of the breeding environment and the harm of diseases have brought huge economic losses to the aquaculture industry.
The traditional method of disease prevention and treatment is mainly to use antibiotics. The use of antibiotics is prone to the problem of drug-resistant strains, and there are residual problems in aquatic animals. Therefore, it is necessary to find alternatives to antibiotics.

Method used

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  • Bacillus licheniformis and feed additive comprising same
  • Bacillus licheniformis and feed additive comprising same
  • Bacillus licheniformis and feed additive comprising same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Isolation, screening and identification of embodiment 1 bacterial strain

[0012] 1. Sample

[0013] It was collected from the bottom mud of the breeding pond of the jiaonan japonicus farm in Jiaonan, Qingdao.

[0014] 2. Culture medium:

[0015] 1) 2216E seawater medium: 5g protein, 1g yeast extract, 0.01g iron phosphate, 1000ml seawater, pH7.6-7.8, prepare solid medium, add 15-20g agar, and sterilize at 121°C for 20min.

[0016] 2) TSB medium: tryptone soybean broth 30g, sodium chloride 15g, distilled water 1000ml, pH7.4-7.6, prepare solid medium, add 15-20g agar, sterilize at 121°C for 20min.

[0017] 3) MRS medium: peptone 10g, beef extract 10g, glucose 20g, yeast powder 5g, Tween-801ml, potassium dihydrogen phosphate 2g, diammonium citrate 2g, sodium acetate 5g, magnesium sulfate 0.58g, manganese sulfate 0.15g , 1000ml of distilled water, pH6.2-6.4, add 15-20g of agar to prepare a solid medium, and sterilize at 115°C for 20min.

[0018] 3. Screening method

[...

Embodiment 2

[0029] Example 2: Effects of Bacillus licheniformis DN29 on the growth, immunity and disease resistance of Apostichopus japonicus

[0030] The experiment set up a control group and a probiotic group, and each group was set up in three parallels. There were 40 juvenile A. japonicus per parallel (initial body weight 0.198±0.008). Among them, the control group uses sargassum powder as the basic feed, and the probiotic group uses 10 9 Add the above-mentioned Bacillus licheniformis DN29 in CFU / g, mix and feed juvenile Apostichopus japonicus. The breeding experiment was carried out in an indoor seawater system for a total of 30 days, feeding twice a day, changing 50% of the water every day, seawater salinity 28-30ppt, pH8.0±0.3, water temperature 20-22℃, and sufficient dissolved oxygen.

[0031] After the breeding experiment, juvenile sea cucumbers were counted and weighed to calculate their specific growth rate. In each parallel, 6 juvenile sea cucumbers were randomly selected, ...

Embodiment 3

[0037] Example 3: Effects of a compound immune enhancer comprising Bacillus licheniformis DN29 on the growth of young sea cucumbers

[0038] In this experiment, a control group and a probiotic group were set up, and three parallels were set up in each group, with 30 juvenile sea cucumbers in each parallel (initial body weight 4.928±0.008). The basal feed containing 21.5% of protein and 4.5% of fat is formulated with sage algae powder, fish meal and flour as basic raw materials. Among them, the control group was fed with basal feed, and the probiotic group was fed with 10% basal feed in the basal feed 9 Add Bacillus licheniformis DN29 for CFU / g, add dextran and peptidoglycan at the ratio of 1.25g / kg and 200mg / kg respectively, mix well and then feed.

[0039] The experiment was carried out in a circulating water system. The experiment lasted for 8 weeks. The sea cucumbers were fed bait at 2-3% of their body weight every day, and the amount of bait was adjusted at any time accor...

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Abstract

The invention relates to the technical field of functional microbiological screening and provides novel Bacillus licheniformis DN29 which is collected under CCTCC NO: M2015482. The Bacillus licheniformis DN29 obtained by screening can effectively inhibit pathogenic bacteria such as Vibrio splendidus and pseudoalteromonas, can reduce disease probability of bred animals and can serve as a feed additive to remarkably increase feed utilization rate of the bred animals and promote growing of the animals. The Bacillus licheniformis DN29 can serve as a probiotic to be applied in the process of rearing and producing Stichopus japonicus, has remarkable promoting effect on growing of Stichopus japonicus and can effectively improve immunity of and resistance, to Vibrio splendidus, of Stichopus japonicus.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Bacillus licheniformis and a feed additive containing the strain. Background technique [0002] With the increase in demand for aquatic products and the international development of aquatic product trade, the aquaculture industry has developed by leaps and bounds in recent years. However, the intensification and high-density scale of aquaculture is expanding day by day, the deterioration of the aquaculture environment and the harm of diseases have brought huge economic losses to the aquaculture industry. The traditional way to prevent and control diseases is mainly to use antibiotics. The use of antibiotics is easy to produce drug-resistant strains, and there are residual problems in aquatic animals. Therefore, it is necessary to find alternatives to antibiotics. [0003] Probiotics can participate in the microbial balance in animals, direc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/80C12R1/10
Inventor 周怡于朝磊许宏伟苟万里陈琳
Owner 青岛玛斯特生物技术有限公司
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