Method for separating denitrification desulfurizing bacteria based on biodiversity information

A technology of denitrification, desulfurization and biodiversity, applied in the field of environmental microbiology, can solve the problems of lack of screening conditions, difficult screening of functional microorganisms, etc., and achieve the effect of efficient and synchronous metabolism of organic carbon

Inactive Publication Date: 2015-03-25
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the blindness of traditional microbial screening methods and the lack of specificity in screening conditions, it is difficult to screen functional microorganisms with different bacterial abundance and spatial ecological distribution in a stable denitrification desulfurization system.

Method used

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  • Method for separating denitrification desulfurizing bacteria based on biodiversity information
  • Method for separating denitrification desulfurizing bacteria based on biodiversity information
  • Method for separating denitrification desulfurizing bacteria based on biodiversity information

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specific Embodiment approach 1

[0023] Specific embodiment one: a kind of method for separating denitrification desulfurization bacteria based on biodiversity information of the present embodiment, it is carried out according to the following steps:

[0024] 1. Sludge samples were collected from a stable expanded granular sludge bed reactor for biological desulfurization and denitrification;

[0025] 2. The high-throughput sequencing technology used to analyze the spatial structure and abundance of the sludge samples to obtain biological diversity information; wherein, the biological diversity information refers to: the fluctuation of bacterial abundance and spatial ecology Distribution information; at the same time, the sludge collected in step 1 was added to sterile water at a volume ratio of 1:5, and glass beads were put in at the same time, and the rotation speed was 220 rpm for 24 hours. suspension of genus;

[0026] 3. After diluting the bacterium solution of each genus in step 2, spread it on the sol...

specific Embodiment approach 2

[0031] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, the sludge sample is collected from the expanded granular sludge bed reactor for biological desulfurization and denitrification in step 1, which refers to the collection of sludge samples from the carbon in organic wastewater. Sludge samples were collected from an expanded granular sludge bed reactor for biological desulfurization and denitrification with removal rates of 99% for pollutants such as nitrogen and sulfur. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0032] Embodiment 3: This embodiment differs from Embodiment 1 in that the sludge collected in step 1 is collected from expanded granular sludge bed reactors that operate stably at different heights for biological desulfurization and denitrification. Others are the same as in the first embodiment.

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Abstract

The invention discloses a method for separating denitrification desulfurizing bacteria based on biodiversity information and relates to denitrification desulfurizing bacterium screening methods. The method comprises the steps of collecting sludge, conducting high-throughput sequencing on the sludge sample, analyzing the flora abundance fluctuation and space ecological distribution of the sludge sample, adopting different screening methods according to community composite and structure characteristics, conducting anaerobic culture on a target bacterial genus by means of an interlayer culture medium with different screening methods, selecting a single colony for isolated culture, and obtaining a single bacterial colony after repeated operation. Two kinds of denitrification desulfurizing bacterial strains, namely autotrophic denitrification desulfurizing bacteria which belong to Thiobacillus, and heterotrophic denitrification desulfurizing bacteria which belong to Thauera, Azoarcus, Arcobacter and Ochrobactrum are obtained through screening. Community structure information displayed by the strain screening result is consistent with that displayed by the high-throughput sequencing result, microbial resources are provided for improving biological treatment effectiveness of wastewater. Furthermore, the metabolic characteristics of the strains are identified.

Description

technical field [0001] The invention belongs to the field of environmental microbiology and relates to a screening method for denitrification and desulfurization bacteria. Background technique [0002] In recent years, with the rapid development of my country's chemical, pharmaceutical, food processing, petroleum refining and other industries, the discharge of high-concentration, refractory organic wastewater has increased year by year. Among them, a considerable part is organic wastewater containing sulfur and nitrogen. Such wastewater contains high concentrations of sulfur and nitrogen compounds, high toxicity, heavy pollution, and serious threats to the environment and human health. It has become one of the main pollution sources that lead to catastrophic ecological damage to water bodies in my country. At present, there are common problems in this kind of wastewater treatment such as "lack of applicable treatment technology, complex process system, huge engineering inve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/68C12Q1/04
CPCC12N1/20C12N1/02
Inventor 王爱杰马晓丹高灵芳远野黄聪谭文博赵友康陈川
Owner HARBIN INST OF TECH
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