Vibrio harveyi secretory type vaccine and structure and application thereof

Abstract

The invention relates to the fields of molecular biology and immunology, in particular to a vibrio harveyi secretory type vaccine and a structure and application thereof. Particularly, the secretory type vaccine is a base sequence in a sequence table SEQ ID No.1, and the construction process thereof is as follows: utilizing vibrio harveyi T4 as a template and F11 and R8 as primers for PCR amplification, connecting the product with pBS-T at room temperature, utilizing NdeI / XhoI double-enzyme cleavage to recover 1.2kb segment from plasmids pBSVhP1, simultaneously utilizing NdeI / XhoI double-enzyme cleavage to recover 4.3kb segment of plasmids pBT3, connecting the two segments at room temperature by ligase T4DNA for 2-4h, transforming connection liquid into colon bacillus DH5 alpha, culturing on an LB solid medium containing Ap for 24h to screen out white transformant, i.e. the Vibrio harveyi secretory type vaccine of base sequence in the expression sequence table SEQ ID No.1. The obtained vaccine has immune and protective functions on Vibrio harveyi. The obtained vaccine has the immune and protective effects on the Vibrio harbeyi, and the immune and protective efficiency of the vaccine of the invention on the Vibrio harveyi can reach to 90%. The preparation process is simple, devitalization and other steps do not needed, and no adjuvant is needed.

Description

technical field

[0001] The invention relates to the fields of molecular biology and immunology, in particular to a vibrio harveyi secreted vaccine and its construction and application. Background technique

[0002] Vibrio harveyi (Vibrio harveyi) is an important pathogenic bacteria in aquaculture, which can infect a variety of aquaculture animals, including fish, shrimp, and shellfish. The current prevention and control of Vibrio harveyi relies on antibiotics and immune control. Due to the potential hazards of antibiotics to the environment and human health, their use has gradually been restricted internationally. Vaccines against Vibrio harveyi are mainly simple inactivated vaccines, and the application of secreted vaccines has not been reported. Contents of the invention

[0003] The purpose of the present invention is to provide a vibrio harveyi secretory vaccine and its construction and application.

[0004] To achieve the above object, the technical solution adopte...