Bacillus pumilus E14, culturing method thereof and application

Abstract

The invention relates to a bacillus pumilus E14 numbered 6682 in China General Microbiological Culture Collection Center (CGMCC). The invention further discloses a culturing method of the bacillus pumilus E14. The method comprises the following steps of: inoculating the bacillus pumilus E14 with a 2216 E liquid medium, and culturing at 28 DEG C and 150 rpm for 20h so as to obtain a bacterial liquid. The bacillus pumilus E14 or the bacterial liquid obtained by the culturing method has a Vibrio harveyi inhibition application, and can be used for preparing shrimp and crab cultivation medicines or is added into shrimp and crab cultivation feeds as the bacterial inhabitation medicine.

Description

technical field [0001] The invention relates to a microbial strain, in particular to a bacillus pumilus E14; the invention also relates to a cultivation method and application of the bacteria. Background technique [0002] In recent years, with the advancement of urbanization and industrialization, the quality of the marine ecological environment has declined year by year, and the breeding diseases have become increasingly serious. They have developed from local breeding areas to almost all breeding areas, and from staged disease to the whole process of breeding. Among them, vibriosis is one of the main bacterial diseases of marine animals, which is caused by bacteria of the genus Vibrio, and Vibrio harveyi is an example. [0003] At present, traditional methods such as chemically synthesized drugs and antibiotics are mostly used in the prevention and treatment of vibriosis, which not only increases bacterial resistance, leads to microbial ecological imbalance, and produces ...

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Problems solved by technology

[0003] At present, traditional methods such as chemically synthesized drugs and antibiotics are mostly used in the prevention and treatment of vibriosis, which not only increases bacterial resistance, leads to microbial ecological imbalance, and produces double infection, but also causes antibiotics to remain in the organism. Ingestion can lead to chronic poisoning, etc., which affects the sustainable development of the aquaculture industry; the abuse of antibiotics has also repeatedly hindered the export of aquatic products in our country, causing huge losses to the aquaculture industry

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