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Bacillus pumilus E14, culturing method thereof and application

A technology of Bacillus pumilus and a culturing method, applied in the field of microbial strains, can solve the problems of blocked aquatic product export, microbial ecological imbalance, affecting the sustainable development of aquaculture industry, etc.

Active Publication Date: 2013-07-17
HUAIHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, traditional methods such as chemically synthesized drugs and antibiotics are mostly used in the prevention and treatment of vibriosis, which not only increases bacterial resistance, leads to microbial ecological imbalance, and produces double infection, but also causes antibiotics to remain in the organism. Ingestion can lead to chronic poisoning, etc., which affects the sustainable development of the aquaculture industry; the abuse of antibiotics has also repeatedly hindered the export of aquatic products in our country, causing huge losses to the aquaculture industry

Method used

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  • Bacillus pumilus E14, culturing method thereof and application
  • Bacillus pumilus E14, culturing method thereof and application
  • Bacillus pumilus E14, culturing method thereof and application

Examples

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Embodiment 1

[0035] Embodiment 1, a kind of bacillus pumilus ( Bacillus pumilus ) E14 CGMCC No. 6682.

Embodiment 2

[0036] Embodiment 2, a method for cultivating Bacillus pumilus E14 as described in Example 1, the steps are as follows: inoculate the Bacillus pumilus E14 strain into 2216E liquid medium, and cultivate it at 28°C and 150 rpm for 20 h to obtain bacteria liquid.

[0037] Bacillus pumilus E14 described in embodiment 1 or the bacteriostatic effect of the bacterium liquid that the method described in embodiment 2 is cultivated can be used for suppressing Vibrio harveyi ( Vibrio Harvey ), which can be used in the preparation of shrimp and crab breeding medicines, or as antibacterial drugs added to shrimp and crab breeding feed.

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Abstract

The invention relates to a bacillus pumilus E14 numbered 6682 in China General Microbiological Culture Collection Center (CGMCC). The invention further discloses a culturing method of the bacillus pumilus E14. The method comprises the following steps of: inoculating the bacillus pumilus E14 with a 2216 E liquid medium, and culturing at 28 DEG C and 150 rpm for 20h so as to obtain a bacterial liquid. The bacillus pumilus E14 or the bacterial liquid obtained by the culturing method has a Vibrio harveyi inhibition application, and can be used for preparing shrimp and crab cultivation medicines or is added into shrimp and crab cultivation feeds as the bacterial inhabitation medicine.

Description

technical field [0001] The invention relates to a microbial strain, in particular to a bacillus pumilus E14; the invention also relates to a cultivation method and application of the bacteria. Background technique [0002] In recent years, with the advancement of urbanization and industrialization, the quality of the marine ecological environment has declined year by year, and the breeding diseases have become increasingly serious. They have developed from local breeding areas to almost all breeding areas, and from staged disease to the whole process of breeding. Among them, vibriosis is one of the main bacterial diseases of marine animals, which is caused by bacteria of the genus Vibrio, and Vibrio harveyi is an example. [0003] At present, traditional methods such as chemically synthesized drugs and antibiotics are mostly used in the prevention and treatment of vibriosis, which not only increases bacterial resistance, leads to microbial ecological imbalance, and produces ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04A23K1/16C12R1/07A61K35/742
Inventor 李联泰安贤惠徐加涛
Owner HUAIHAI INST OF TECH
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