Agricultural insecticidal composition containing Verticillium lecanii
A technology of Verticillium leek and an insecticidal composition, applied in the field of pesticides, can solve the problems of not being at the same level and being unsatisfactory, and achieve the effects of reducing residues, reducing the dosage of pesticides, and reducing environmental pollution
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Embodiment 1
[0023] Embodiment 1 Indoor bioactivity assay
[0024] Experiment 1: Determination of indoor activity of Verticillium lecanii and indoxacarb against tea leafhoppers
[0025] Test object: tea leafhopper
[0026] Test agent: Verticillium lecanii K1207 (5×10 9 live spores / gram), indoxacarb (95%)
[0027] Test method (leaf soaking method): Put the young tea tree leaves with infestation in the refrigerator for 5-10 minutes, take them out when they are inactive, select the young tea tree leaves with more infestation, and remove the young nymphs and adults. Soak the young leaves of Luochong tea tree in the liquid medicine for 10 seconds, dry the base with cotton balls to keep moisture, put them in a straight cylindrical glass insect cover, cover the upper opening with 80-mesh nylon net, and tie it tightly with a rubber band. Each treatment was repeated 4 times, and 20 tea leafhoppers were used for each repetition. After the treatment, they were kept at (26±1)°C, light:dark=14h:10h...
Embodiment 2
[0040] Embodiment 2: formulation embodiment
[0041] The following examples use 5.0×10 9 Live spores / gram Verticillium laxacilis spore powder, 95% indoxacarb technical, 95% flubendiamide, and the percentages of each component are percentages by weight.
[0042] Preparation 1: 30% Verticillium lecanii · Indoxacarb SC / 30% Verticillium lecanii · Flubendiamide SC
[0043] 1% Verticillium lecanii spore powder, 29% indoxacarb / flubendiamide, 10% sorbitan fatty acid ester polyoxyethylene ether, 5% sodium lauryl sulfate, 3% tert-butyl p- Hydroquinone, ethoxylated vegetable oils make up to 100%.
[0044] Add the indoxacarb, sorbitan fatty acid ester polyoxyethylene ether, sodium lauryl sulfate, tert-butyl hydroquinone and ethoxylated vegetable oil into the mixing kettle in sequence, stir evenly, and After shearing in the shearing and dispersing kettle, it enters the sand mill for sand milling, and after sand milling until 90% of the particle size is less than 5 microns, it is evenly ...
Embodiment 3
[0054] Embodiment three: Drug efficacy test
[0055] Test 1: Control Test of Tea Leafhopper
[0056] Use the spray method. In the experiment, 3 tea trees with tea leafhoppers were selected. The number of tea leafhoppers was counted before the drug treatment, and the number of live insects of the tea leafhoppers was investigated 3 days, 7 days, 10 days, and 14 days after the drug treatment, and the control effect was calculated.
[0057] Table 3 The efficacy test of the combination of Verticillium lecanii and indoxacarb against green leafhoppers
[0058]
[0059] The combination of Verticillium lecanii and indoxacarb for the control of tea leafhoppers has improved quick-acting and persistent effects, which is of great significance for reducing the amount of pesticides and alleviating drug resistance.
[0060] Experiment 2: Control experiment of diamondback moth
[0061] During the peak period of Plutella xylostella larvae (mainly 2-3 instars), the growth of cabbage is in ...
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