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Insecticide jointly using matrine and verticillium lecanii

A technology of Verticillium leek, matrine, which is applied in the directions of pesticides, applications, biocides, etc., can solve the problems of resistance, insignificant drug effect, etc., and achieves delaying the generation of insect-resistant types of pests and improving the control effect. , the effect of reducing environmental pollution

Active Publication Date: 2017-05-31
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to overcome the above-mentioned problems such as the existing pesticides are easy to produce resistance and the drug effect is not obvious, and to provide a composition of matrine and Verticillium lecanii with synergistic effect. The research on the compound effect of matrine and Verticillium lecanii and the activity of acetylcholinesterase (AchE) after treatment found that the compound compound can show unexpected synergistic effect, improve the control effect, and can obviously Reduce the dosage of single chemical pesticides and reduce environmental pollution

Method used

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  • Insecticide jointly using matrine and verticillium lecanii
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  • Insecticide jointly using matrine and verticillium lecanii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Indoor Toxicity Determination of the Combination of Verticillium lecanii and Matrine to Bemisia tabaci

[0028] 1. Experimental materials

[0029] Verticillium lecanii: Verticillium lecanii L. muscarium V20, preserved in the Biological Control Engineering Research Center of the Ministry of Education, South China Agricultural University, was originally isolated from the infected whitefly. The initial concentration of Verticillium lecanii spore suspension that the present invention uses is 1 * 10 8 / ml, diluted to a lower concentration by stepwise dilution method for experiments.

[0030] Matrine powder (95%): provided by Guangdong Yangjiang New City Bioengineering.

[0031] 2. Experimental method

[0032] (1) Drug grouping: matrine, Verticillium lecanii, combination of matrine and Verticillium lecanii. The combinations of Verticillium lecanii, matrine and their different concentration ratios used in experiments in each group are shown in Table 1.

[003...

Embodiment 2

[0043]Example 2 Determination of sensitivity to matrine after Verticillium lecanii treated Bemisia tabaci for 24 hours

[0044] 1. As shown in Table 3, treat newly molted 2nd instar Bemisia tabaci nymphs with different concentrations of Verticillium lecanii. The nymphs treated on the leaves were placed in a clean glass Petri dish (φ20 cm). Place a piece of filter paper on the bottom of the dish and keep hydrated with 200 μl of water. After 24 hours, leaves with whitefly nymphs infected with Verticillium lecanii were immersed in different concentrations of matrine (as shown in Table 3) for 30 seconds. The same batch of insect and conidia suspensions was used for each treatment group (each dose combination) and control group, with 3 replicates, each replicate containing 4 leaves, 100 Bemisia tabaci nymphs per leaf. Mortality was recorded for 8 days at 24 hour intervals.

[0045] Table 3 Different concentrations and ratios of Verticillium lecanii and matrine used in the experi...

Embodiment 3

[0053] Example 3 Determination of acetylcholinesterase activity of different treatments in different time intervals in Bemisia tabaci

[0054] 1. Use different concentrations of Verticillium lecanii (1×10 6 , 1×10 7 and 1×10 8 conidia / ml), matrine (5, 0.5 and 0.05mg / L) and Verticillium lecanii+matrine combination (5mg / L+1×10 8 Conidia / ml, 0.5mg / L + 1×10 7 Conidia / 0.05mg / L + 1×10 6 conidia / ml) inoculated 2nd instar Bemisia tabaci nymphs (100 heads). Collected nymphs were homogenized with ice-cold 0.05 M sodium phosphate buffer (pH 7.0). Homogenized samples were centrifuged at 12000 rpm for 10 minutes at 4°C. Transfer the supernatant to a new tube and centrifuge at 12000 rpm for 15 min at 4 °C. The final supernatant was used as enzyme preparation.

[0055] Determination of acetylcholinesterase (AChE) activity: The reaction mixture contained 50 μL of sample solution, 100 μL of 45 μM 5-5-dithiobis(2-nitrobenzoic acid), 100 μL of acetylthiocholine iodide, and 90 μL of sodi...

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Abstract

The invention discloses an insecticide jointly using matrine and verticillium lecanii. The insecticide uses matrine and verticillium lecanii as main active components. The insecticide has the advantages that the synergistic action of composition of matrine and verticillium lecanii is obvious, the usage amounts of matrine and verticillium lecanii are reduced, the use cost is reduced, the environment-friendly effect is realized, the drug resistance is not produced, and the strong application potential is realized in the biological prevention and control of bemisia tabaci.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to an insecticide combined with matrine and Verticillium lecanii. Background technique [0002] Bemisia tabaci ( Bemisiatabaci (Gennadius)) is a worldwide pest with a wide range of hosts. In addition to direct food damage, it also secretes honeydew that affects photosynthesis, pollutes crop quality, and spreads plant viruses and induces plant virus diseases. The global average annual economic loss More than $300 million. Bemisia tabaci was recorded in my country in the 1940s, but it has not been listed as a major pest because of its mild damage. In recent years, the B-biological type Bemisia tabaci, which is more harmful, has invaded my country and spread rapidly to more than 20 provinces and cities. It broke out and caused disasters in many areas, causing major economic losses to vegetable and flower production. [0003] At present, the control of Be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/90A01P7/04
CPCA01N43/90A01N63/30A01N2300/00
Inventor 邱宝利阿里·肖卡特郭长飞王兴民
Owner SOUTH CHINA AGRI UNIV
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