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Selective culture medium for quantitatively detecting verticillum lecanii and application thereof

A technology for quantitative detection of Verticillium lecanii, applied in the field of microorganisms, can solve the problems of restricting application and promotion, unfavorable use methods of Verticillium lecanii, lack of Verticillium lecanii, etc., and achieves low price, simple preparation, The effect of easy access to components

Inactive Publication Date: 2014-12-17
张艳军 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the basic research on the continuation and survival of Verticillium lecanii in the field is quite lacking, which is not conducive to specifying suitable methods for the use of Verticillium lecanii, which seriously restricts the application and promotion of this fungus.

Method used

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  • Selective culture medium for quantitatively detecting verticillum lecanii and application thereof
  • Selective culture medium for quantitatively detecting verticillum lecanii and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Embodiment 1: a kind of selective culture medium of quantitative detection Verticillium lecanii, is characterized in that: oat 15g, pours in the pot, adds distilled water and boils 30 minutes, removes residue with 4 layers of gauze filtration, and filtrate adds agar powder 17g and cetyltrimethylammonium bromide 0.5g, boiled again, distilled water to 1000ml, 121 ℃ autoclaving for 25min, when the culture medium is cooled to not hot, add chloramphenicol 0.5g, mix well Finally, pour it into sterilized Petri dishes with a diameter of about 9cm, about 20mL per dish, and store at low temperature after condensation for later use, and the storage time should not exceed 2 weeks.

Embodiment 2

[0014] Embodiment 2: A kind of selective culture medium of quantitative detection Verticillium lecanii, is characterized in that: oat 15g, pours in the pot, adds distilled water and boils 30 minutes, removes residue with 4 layers of gauze filtration, and filtrate adds agar powder 17g and carbendazim 0.5g, boiled again, distilled water to 1000ml, 121 ℃ autoclaving for 25 minutes, when the culture medium was cooled to not hot, add streptomycin sulfate 0.5g, after mixing well, pour into the sterilized Bacteria in a petri dish with a diameter of about 9cm, each dish is about 20mL, and stored at low temperature after condensation for later use, and the storage time does not exceed 2 weeks.

Embodiment 3

[0015] Embodiment 3: A kind of selective culture medium of quantitative detection Verticillium lecanii, is characterized in that: oat 15g, pours in the pot, adds distilled water and boils 30 minutes, removes residue with 4 layers of gauze filtration, and filtrate adds agar powder 17g , Hexadecyltrimethylammonium bromide 0.3g and carbendazim 0.3g, boil again, distilled water to 1000ml, 121 ℃ autoclaving for 25min, when the culture medium is cooled to not hot, add chloramphenicol 0.5g, mix well, pour into sterilized Petri dishes with a diameter of about 9cm, about 20mL per dish, store at low temperature after condensation, and store for no more than 2 weeks.

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Abstract

The invention discloses a selective culture medium for quantitatively detecting verticillum lecanii and an application thereof. The selective culture medium for quantitatively detecting verticillum lecanii is prepared from the following components by weight: 10-20g of oatmeal, 15-20g of agar powder, 0.1-1.0g of bacterial bacteriostatic agent, 0.1-1.0g of fungus bacteriostatic agent and 1000ml of distilled water. The selective culture medium for quantitatively detecting verticillum lecanii has the advantages that interference of bacteria to target fungus detection is fully expelled by means of an inhibiting and killing effect of antibiotics to bacteria; influence of other funguses in a to-be-detected sample is eliminated by means of characteristics of sensitivity difference of different funguses on the fungus bacteriostatic agent as well as the difference of different funguses in utilization capacity of a carbon source, thereby achieving the purpose of effectively and quantitatively detecting verticillum lecanii. The culture medium is easily available in component, low in price, simple to prepare and convenient to use.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a selective culture medium for quantitatively detecting Verticillium lecanii and its application. Background technique [0002] Verticillium lecanicillium (Lecanicillium Lecanii) is an important entomogenic fungus, which has a good control effect on piercing-sucking pests. This fungus has been widely used in many countries and regions in Western Europe, the Soviet Union, the United States, and the Middle East. As we all know, the control effect of bio-control bacteria on crop pests is closely related to the application time, single application dose, and application frequency of bio-control bacteria. Therefore, it is particularly important to formulate appropriate methods of bio-control bacteria application for different crops or habitats. Biocontrol bacteria exist naturally in nature, and it is of great significance to guide the use of biocontrol bacteria to study the na...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 张艳军谢明彭德良
Owner 张艳军
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