Compound desinsection primary agent and pesticide consisting of verticillium lecanii and nimbin

A technology of Verticillium lecanii and azadirachtin, applied in the field of pesticides, can solve the problems of limited resources of azadirachtin, low supply of azadirachtin preparations, and no discovery of Verticillium lecanii and azadirachtin. Delaying the emergence of pest resistance, strong infection and insecticidal effect, and the effect of reducing pesticide residues

Inactive Publication Date: 2009-08-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the resources of azadirachtin are limited, and the supply of azadirachtin preparations is far lower than the demand in production
At present, there is no relevant technical report on the compound insecticide of Verticillium lecanii and azadirachtin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: 20% Verticillium lecanii azadirachtin suspension concentrate (diluted 2000 times during use)

[0024] Part A is 40% conidia suspension of Verticillium lecanii: 40% conidia powder of Verticillium lecanii is dissolved in 0.21M phosphate buffer and 10% glycerin mixture, adjust pH to 7.0, Store at 4-8°C.

[0025] Part B is wettable powder: azadirachtin (commercially available) 0.12%, Tween-80 20%, sodium lignosulfonate 6%, wetting agent LX 4%, add clay to make up to 100%.

[0026] Part A and Part B each account for 50%, and the two parts are directly mixed to obtain 20% Verticillium lecanii azadirachtin suspension concentrate, and the mass ratio of Verticillium lecanii:Azadirachtin is 100:0.30. The results of the toxicity test are shown in Table 1.

Embodiment 2

[0027] Embodiment 2: 50% Verticillium lecanii azadirachtin oil (diluted 2000 times during use)

[0028] Verticillium lecanii powder (10 10 each / g) 50%, azadirachtin 0.1% (commercially available), Tween-805%, paraffin oil 23%, methyl green 2%, add diatomaceous earth to make up to 100%, and make 50% wax scale Azadirachtin oil. Among them, the mass ratio of Verticillium lecanii bacterial powder to azadirachtin is 100:0.2. The results of the toxicity test are shown in Table 1.

Embodiment 3

[0029] Embodiment 3: 60% Verticillium lecanii azadirachtin oil (diluted 2000 times during use)

[0030] Verticillium lecanii powder (10 10 Individual / g) 60%, azadirachtin 0.05% (commercially available), methyl green 2%, Tween-805%, paraffin oil 23%, kaolin supplemented to 100%, and 60% Verticillium lecanii imprint Neem oil. Wherein, the mass ratio of Verticillium lecanii bacterial powder:Azadirachtin is 100:0.08. The results of the toxicity test are shown in Table 1.

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PUM

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Abstract

The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and nimbin. The primary agent is a mixture of the verticillium lecanii and the nimbin, wherein, the mass ratio between the verticillium lecanii and the nimbin is 100:0.05-0.30; while the pesticide consists of a primary agent and a secondary agent; according to mass percent, the primary agent accounts for 20-60%, and the secondary agent 40-80%. The compound pesticide of the invention can be prepared into wettable powder, fungal spore suspending agent and medicinal oil; in addition, the compound pesticide of the invention has better effect in preventing and controlling the pests than the preventing effect when one medical preparation alone such as the nimbin is used and can effectively reduce the use of the nimbin in practical production. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pests in particular. The compound pesticide consisting of the verticillium lecanii and the nimbin of the invention has good effect in preventing and controlling pests such as white fly, plant louse, diamond back moth and the like.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and in particular relates to a microbial insecticide, in particular to an environmentally friendly, pollution-free and residue-free insecticide main agent and insecticide compounded with Verticillium lecanii and azadirachtin. Background technique [0002] Verticillium lecanii (Zimmermann) Viegas is an entomopathogenic fungus. As a living microbial pesticide, it has a new mechanism of action different from existing chemical insecticides, and has the characteristics of no pollution to the environment and no residue. , can be used as an alternative control drug to control some insects resistant to Bacillus thuringiensis (Bt), and the development and utilization of Verticillium lecanii has important economic significance. Verticillium lecanii is widely distributed, and has good control effects on whitefly, greenhouse whitefly, aphids, thrips, etc., and has broad development and application prospec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/90A01N65/00A01P7/00A01P7/04
CPCY02A50/30
Inventor 黄振任顺祥
Owner SOUTH CHINA AGRI UNIV
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