Bacillus licheniformis 201 and use thereof

A technology of Bacillus licheniformis and mycelium, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of blank and weak technology, and achieve the effects of easy preservation, good development and application prospects, and simple cultivation conditions

Inactive Publication Date: 2009-07-22
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Isolation and screening from banana plants to obtain biocontrol bacterial strains with antagonistic effects on Fusarium wilt of banana is of great significance to the control of Fusarium wilt of banana, but the relevant technology is still weak or even blank

Method used

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  • Bacillus licheniformis 201 and use thereof
  • Bacillus licheniformis 201 and use thereof
  • Bacillus licheniformis 201 and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Inhibitory effect of Bacillus licheniformis 201 on mycelia FOC4 of Fusarium wilt fungus No. 4 race

[0027] Insert the 5mm diameter mycelium block of mycelium FOC4 of the banana fusarium wilt fungus race 4 into the center of the PDA plate of potato sucrose medium, and inoculate three pieces of sterilized filter paper with a diameter of 5mm around the FOC4 mycelium block at equal distances. 10 μL of culture solution of strain 201 cultured for 24 h was added dropwise on the filter paper sheet. Repeat three times, culture at 25-28°C, and measure the diameter of the inhibition zone after 5 days. attached by figure 1 It can be seen that Bacillus licheniformis 201 has a strong antibacterial effect on Fusarium wilt of banana. Example 2 Inhibition of Bacillus licheniformis 201 on Banana Fusarium Wilt under Greenhouse Conditions

Embodiment 2

[0028] The Brazilian banana seedlings with a plant height of about 10 cm were immersed in the culture solution of the strain 201, and the OD of the culture solution was equal to 0.5. After 30 minutes, they were taken out and planted in flower pots. On the 2d, 7d, 15d and 30d respectively, pick up the banana seedlings, immerse them in the spore suspension of FOC4 for 30min, the spore suspension is 1×105 spores / mL, and then plant them in flower pots. Treat 36 plants in pots. After inoculation, the banana seedlings were placed in a greenhouse at 23-25°C for cultivation, and watered once a day. The incidence rate and disease index were investigated 20 days, 30 days and 40 days after FOC4 inoculation. The results showed that the challenge inoculation with FOC4 after strain 201 was inoculated 15 days and 30 days later, compared with the control, the incidence of banana seedlings was significantly reduced. 82% and 57%, while the incidence rate of the control strain was above 95%; t...

Embodiment 3

[0029] Example 3 Teratogenic effect of Bacillus licheniformis 201 on FOC4 hyphae

[0030] Cut the cellophane into strips of 5mm×25mm, autoclave at 121°C, and place it between the strain 201 and the pathogen FOC4 with tweezers. Cultivate at a constant temperature of 28°C for 5 to 7 days. After an obvious bacteriostatic zone appears between the mycelium of strain 201 and FOC4, carefully remove the cellophane and observe the morphological changes of the mycelium under a microscope with a power of 10×40. The pathogenic bacteria of strain 201 was used as a control. It is observed that the hyphae are enlarged and prominent in the early stage, and obviously thinner in the later stage, with many branches and short, and uneven shape. The teratogenic effect of Bacillus licheniformis 201 on FOC4 mycelium can be understood by observing the morphological changes of the mycelium under a 10×40 magnification microscope : The experimental results are attached Figure 2-4 , with figure 1 It...

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Abstract

The invention discloses a Bacillus licheniformis 201, which is preserved in China Center for Type Culture Collection that is located in Wuhan University, Logosun, Wuchang, Wuhan, Hubei, and with CCTCC NO. of M207092. The invention simultaneously discloses a preparation method and applications of the Bacillus licheniformis 201, which has strong bacteriostasis of banana wilt fusarium, wide bacteriostasis spectrum and antagonism to various pathogenic fungi including banana wilt fusarium, watermelon wilt fusarium, tomato wilt fusarium, banana colletotrichum, gloeocystidium colletotrichum, corn leaf spot curvularia, cotton verticillium lecanii, gibberella zeae, and the like, and the Bacillus licheniformis 201 has high control efficiency, good environmental security and good development and application prospect.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to an endogenous biocontrol bacterial strain, in particular to a bacillus licheniformis 201 for efficiently preventing and treating banana wilt and its application. Background technique [0002] Banana Fusarium wilt is a devastating disease caused by the infection of Fusarium oxysporum f.sp.cubense, and it is a devastating soil-borne disease of banana production. The pathogen invades from the injured rhizome, spreads to the pseudostem and leaves through the vascular bundle, and blocks the xylem vessel until the plant withers and dies. Fusarium wilt of banana is a soil-inhabiting fungus, and its chlamydospores can survive in the soil for 8 to 10 years. Banana Fusarium wilt is distributed in many counties and cities in the banana production area of ​​the Pearl River Delta in China. The rate of diseased plants in the diseased garden is about 10% to 40%, and it is more than 90% in sever...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/10
Inventor 王振中王春华纪春艳孙正祥
Owner SOUTH CHINA AGRI UNIV
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