Predatory mite and verticillum lecanii combined-application biopesticide and preparing and application method thereof

A technology of Verticillium lecanii and biopesticides, applied in the direction of botanical equipment and methods, applications, acaricides, etc., can solve the problems of large manpower and material resources, achieve uniform distribution, save manpower and material resources, and protect the ecological environment

Inactive Publication Date: 2015-10-21
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Both spider mites and whitefly pests are important pests in plants, and they often occur together on many crops. The current method is generally to control them separately, which consumes a lot of manpower and material resources.

Method used

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  • Predatory mite and verticillum lecanii combined-application biopesticide and preparing and application method thereof
  • Predatory mite and verticillum lecanii combined-application biopesticide and preparing and application method thereof
  • Predatory mite and verticillum lecanii combined-application biopesticide and preparing and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1. The predation effect of releasing Neoseius pasterei alone on Panonychus citrus

[0023] 1. Predation and control effects of different methods of use on Panonychus citrus

[0024] The test was carried out in the navel orange orchard of the Citrus Research Institute of the Chinese Academy of Agricultural Sciences in Xiema Town, Beibei District, Chongqing City, and the area of ​​each demonstration area was 1-3hm 2 , the demonstration area is equipped with a spreading area (1,500 neoseiusius mite per tree), a release area (1,500 neoseiusius mite hanging from each tree), and a chemical defense area (that is, 15% pyridaben 30ml / 667m 2 ), the blank control area. Set up 10 standard plants in each district, and each plant is divided into 5 orientations, east, west, north, south, and middle, and 10 leaves are fixedly observed in each orientation, with 50 leaves per plant. effect.

[0025] The control effect of Neoseiius pasteurii on Panonychus citrus is shown i...

Embodiment 2

[0040] Example 2. Effect of Verticillium lecanii on Neoseiusius mite

[0041] Use a petri dish with a diameter of 15 cm, put a sponge with a built-in diameter of 13 cm and absorb water at the bottom of the petri dish, put a piece of filter paper on the sponge, and then put the washed and dried citrus leaves on the filter paper, and cover the edges of the leaves with cotton The thin strips form a thin circle to prevent the escape or inward migration of spider mites and predator mites. Inoculate 50 adult mite of Neoseiusius pastereii on each plate, and put in enough Panonychus citrus to carry out the test.

[0042] In order to help the germination of Verticillium lecanii spores, this experiment adopts the spore powder spraying method: take a certain amount of spore powder, respectively configured into the following spore powder suspension (5 × 10 6 pcs / ml, 4×10 6 pcs / ml, 3×10 6 pcs / ml, 2×10 6 pcs / ml, 1×10 6 Individual / ml) spore powder bacterial liquid of 5 concentrations, s...

Embodiment 3

[0047] Example 3. Effect of Verticillium lecanii controlling citrus whitefly alone

[0048] Before the peak of the first generation of citrus whiteflies (the peak of occurrence is at the end of April), and at the beginning of March, the outdoor potted seedlings (over 1 year old, because citrus whiteflies like to harm young shoots and leaves) are pruned intensively. , put the shoots once, and then put the pruned potted seedlings in the middle of the field, and adopt the method of natural inoculation, so that the new shoots and leaves of the potted seedlings are infected with citrus whitefly in large quantities. When the second-instar nymphs of the citrus whitefly appeared in large numbers, the potted seedlings infected with the citrus whitefly were collected in a large airtight net room for natural feeding of the whiteflies for the test. During the test, the leaves of potted seedlings with a sufficient number of second-instar citrus whiteflies that had neatly developed for the ...

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Abstract

The invention discloses a predatory mite and verticillum lecanii combined-application biopesticide and a preparing and application method thereof and belongs to the field of plant insect pest prevention. The predatory mite and verticillum lecanii combined-application biopesticide is prepared by wheat bran, predatory mites and verticillum lecanii conidial powder in a mixed mode. According to the ratio, each kilogram of wheat bran is mixed with 1.5 * 105-2.0 * 105 predatory mites and 3-5 grams of verticillum lecanii conidial powder. The preparing method comprises the steps that 1. materials are prepared, wherein the verticillum lecanii conidial powder and the predatory mites are produced; and 2. the predatory mites and the verticillum lecanii conidial powder are mixed. The application method comprises the steps that on March to April or September to October each year, the combined biopesticide is applied in a broadcasting or powder spraying mode, so that the pesticide is evenly distributed on leaves, according to the using amount, 8-12 grams are sprayed to fruit trees at least 5 years old, 4-6 grams are sprayed to fruit trees at most 5 years old, and 450-550 grams are sprayed to each acre of vegetable and flowers.

Description

technical field [0001] The invention belongs to the field of plant pest control, and relates to biological pesticides and their application to control pests, in particular to biological pesticides combined with predatory mites and Verticillium lecanii and their preparation and application methods. Background technique [0002] Predator mite is a kind of omnivorous beneficial mite whose main food is citrus red spider mite, yellow spider mite, rust tick, hawthorn spider mite, cinnabar spider mite, apple spider mite and other plant spider mites. Predator mite is used to control and control citrus Orange red spider, yellow spider and rust tick can replace chemical control to control citrus pest mites, and it is one of the important technologies to produce pollution-free and green citrus. Predatory mite is a general term for many beneficial mites, and its scope is very wide, including Echomidae, Echromidae, Pleurodidae, Pyrethidae, and Phytoseiidae. At present, the predatory mit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P7/02A01P7/04A01G13/00
Inventor 李鸿筠刘浩强冉春丛林
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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