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Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control

A technology of Verticillium leek, oil suspension agent, applied in the directions of botanical equipment and methods, application, pesticides, etc., can solve the problems of affecting fungal spore viability, high environmental humidity requirements, unstable field control effect, and the like, Achieve the effect of overcoming severe dependence on high humidity, good compatibility, and promoting spore germination

Inactive Publication Date: 2015-01-07
谢明 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wettable powders are easy to use and have a wide range of control, but the surfactants added in the preparations often affect the viability of fungal spores. Like powders, wettable powders require high environmental humidity, and the control effect in the field is unstable.

Method used

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  • Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control
  • Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control
  • Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: The influence of emulsifier on the spore activity of Verticillium lecanii in oil suspension

[0014] Grind soybean oil, Verticillium lecanii spore powder (20% by weight) and emulsifier in a grinder for 10 minutes (1000 rpm), the temperature of the whole process must not be higher than 38°C, and each configuration is 500 grams different The oil suspension of the emulsifier should be stored in a ground Erlenmeyer flask at room temperature. Mix the different oil suspensions thoroughly every month, then use the inoculation loop to inoculate a little on the 2% water agar plate (WA), spread evenly, incubate at a constant temperature of 25±1°C, check under the microscope after 16 hours, and count 200 - 300 spores, calculate the germination rate. The dried spore powder of Verticillium lecanii (water content less than 5%) stored in a sealed storage at 4°C was used as a control, and each treatment was repeated 5 times. It can be seen from Table 1 that after the sp...

Embodiment 2

[0018] Example 2: Control effect of Verticillium lecanii oil suspension concentrate on apple yellow aphid

[0019] Grind rapeseed oil, Verticillium lecanii spore powder (20% by weight) and emulsifier in a grinder for 10 minutes (1000 rpm), the temperature of the whole process must not be higher than 38°C, and each configuration is 500 grams Oil suspensions of different emulsifiers should be stored in ground-mouth Erlenmeyer flasks at room temperature. Different Verticillium lecanii oil suspensions were diluted 1000 times with water, the conventional drug was 25% deltamethrin emulsifiable concentrate, and the blank control was clear water. Carry out insect population base survey before spraying. After the survey, spray the whole apple tree evenly with a knapsack manual sprayer. The spraying rate is 2250kg / hm 2 , Control efficacy investigations were carried out on the 10th day, 20th day and 30th day after the medicine. Trials were repeated 3 times for each treatment setting. ...

Embodiment 3

[0023] Example 3: Control effect of Verticillium lecanii oil suspension concentrate on alfalfa aphids

[0024] Grind soybean salad oil, Verticillium lecanii spore powder (20% by weight) and emulsifier in a grinder for 10 minutes (1000 rpm), the temperature of the whole process must not be higher than 38°C, and each configuration is 500 grams Oil suspensions of different emulsifiers should be stored in ground-mouth Erlenmeyer flasks at room temperature. Different Verticillium lecanii oil suspensions were diluted 1000 times with water, the conventional drug was 40% omethoate EC, and the blank control was clear water. The variety of alfalfa is Canadian ABLE, and the main types of alfalfa aphids are pea aphid without net and clover aphid. The experiment was set up at the Experimental Farm of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Langfang, Hebei. During the peak period of alfalfa aphid occurrence, use a knapsack sprayer to evenly spray each ...

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Abstract

The invention discloses a lecanicillium lecanii oil solution, a preparing method thereof and applications of the oil solution in agricultural pest control. A lecanicillium lecanii oil suspension is prepared from following components by weight: 10-30% of lecanicillium lecanii spore powder, 5-20% of an emulsifier, 1-3% of a stabilizing agent and 47-84% of plant oil. The oil suspension is advantageous in that: the spore storage time is long, and after storage at room temperature for 15 months, the rate of live spores is more than 80%; the cost is low, and the amount of oil used for dilution of the oil solution is largely saved; the oil suspension meets conventional pesticide using custom; operation in the field is simple, wherein the oil suspension is directly diluted with water by 1-10 times and then sprayed by an ultra-low volume technology, or is diluted with water by 1000-2000 times and then sprayed in a normal amount; and in a dry environment, the product has good control effects for apple yellow aphids and alfalfa aphid.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a lecanococcus oil agent, a preparation method thereof and its function in preventing and controlling agricultural pests. Background technique [0002] Piercing-sucking pests (aphids, whiteflies, thrips, etc.) are important pests of a variety of crops, and their control mainly depends on chemical pesticides. Moreover, excessive pesticide residues in agricultural products are also becoming more and more serious. In recent years, people's living standards have been continuously improved, and the requirements for food quality are getting higher and higher, and the production of pollution-free food and green food has emerged as the times require. The use of bio-pesticides that do not pollute the environment and are non-toxic to humans and animals to replace chemical pesticides has become an important means of crop pest control. Verticillium lecanicillium (L...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/04A01P7/04
Inventor 谢明张艳军陈福良彭德良尉文彬
Owner 谢明
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