Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control

A technology of Verticillium leek, oil suspension agent, applied in the directions of botanical equipment and methods, application, pesticides, etc., can solve the problems of affecting fungal spore viability, high environmental humidity requirements, unstable field control effect, and the like, Achieve the effect of overcoming severe dependence on high humidity, good compatibility, and promoting spore germination

Inactive Publication Date: 2015-01-07
谢明 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wettable powders are easy to use and have a wide range of control, but the surfactants added in the preparations often affect the viabili

Method used

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  • Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control
  • Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control
  • Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0013] Example 1: Influence of emulsifier on spore viability of Verticillium leek in oil suspension

[0014] Grind soybean oil, Verticillium cereus spore powder (20% by weight) and emulsifier in a grinder for 10 minutes (1000 rpm), the temperature during the whole process should not be higher than 38 ℃, and each configuration is 500 grams different The oil suspension of the emulsifier is stored in a ground conical flask at room temperature. Mix different oil suspensions thoroughly every month, then dip a little bit with an inoculating loop and inoculate it on a 2% water agar plate (WA), spread evenly, incubate at 25±1°C, microscopically examine after 16 hours, and count 200 -300 spores, calculated germination rate. The dry spore powder of Verticillium cereus (water content less than 5%) stored in a low temperature seal at 4°C was used as a control, and each treatment was repeated 5 times. As can be seen from Table 1, after the spores of Verticillium cereus were stored in the...

Example Embodiment

[0018] Example 2: Control effect of Verticillium leeks oil suspending agent on yellow apple aphid

[0019] Grind rapeseed oil, Verticillium cereus spore powder (20% by weight) and emulsifier in a grinder for 10 minutes (1000 rpm), the temperature during the whole process should not be higher than 38 ℃, and each configuration is 500 grams Oil suspensions of different emulsifiers are stored in ground conical flasks at room temperature. The oil suspensions of different Verticillium cereus were diluted 1000 times with water, the conventional agent was 25% deltamethrin EC, and the blank control was clear water. The insect population base was investigated before spraying. After the investigation, the whole apple tree was evenly sprayed with a knapsack hand sprayer, and the spraying rate was 2250kg / hm. 2 , the control effect was investigated on the 10th, 20th and 30th days after the drug. Trials were set up with 3 replicates per treatment. From Table 1, it can be seen that the oil...

Example Embodiment

[0023] Example 3: Control effect of Verticillium leeks oil suspension on alfalfa aphids

[0024] Grind the soybean salad oil, Verticillium cereus spore powder (20% by weight) and emulsifier in a grinder for 10 minutes (1000 rpm), the temperature during the whole process should not be higher than 38 ℃, and each configuration should be 500 grams Oil suspensions of different emulsifiers are stored in ground conical flasks at room temperature. Different Verticillium cereus oil suspending agents were diluted 1000 times with water, the conventional agent was 40% omethoate EC, and the blank control was clear water. The alfalfa variety is Canadian ABLE, and the alfalfa aphid species are mainly aphid of peas without nets and aphids of clover. The experiment was set up in the experimental farm of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Langfang, Hebei Province. In the peak period of alfalfa aphids, use a knapsack sprayer to evenly spray each treatm...

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Abstract

The invention discloses a lecanicillium lecanii oil solution, a preparing method thereof and applications of the oil solution in agricultural pest control. A lecanicillium lecanii oil suspension is prepared from following components by weight: 10-30% of lecanicillium lecanii spore powder, 5-20% of an emulsifier, 1-3% of a stabilizing agent and 47-84% of plant oil. The oil suspension is advantageous in that: the spore storage time is long, and after storage at room temperature for 15 months, the rate of live spores is more than 80%; the cost is low, and the amount of oil used for dilution of the oil solution is largely saved; the oil suspension meets conventional pesticide using custom; operation in the field is simple, wherein the oil suspension is directly diluted with water by 1-10 times and then sprayed by an ultra-low volume technology, or is diluted with water by 1000-2000 times and then sprayed in a normal amount; and in a dry environment, the product has good control effects for apple yellow aphids and alfalfa aphid.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a lecanococcus oil agent, a preparation method thereof and its function in preventing and controlling agricultural pests. Background technique [0002] Piercing-sucking pests (aphids, whiteflies, thrips, etc.) are important pests of a variety of crops, and their control mainly depends on chemical pesticides. Moreover, excessive pesticide residues in agricultural products are also becoming more and more serious. In recent years, people's living standards have been continuously improved, and the requirements for food quality are getting higher and higher, and the production of pollution-free food and green food has emerged as the times require. The use of bio-pesticides that do not pollute the environment and are non-toxic to humans and animals to replace chemical pesticides has become an important means of crop pest control. Verticillium lecanicillium (L...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/04A01P7/04
Inventor 谢明张艳军陈福良彭德良尉文彬
Owner 谢明
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