High-oleic-acid-content peanut molecular marker, assistant selection back cross breeding method and application of back cross breeding method

Abstract

The invention relates to a high-oleic-acid-content molecular marker, a back cross breeding method for carrying out assistant selection by utilizing the molecular marker, and an application of the back cross breeding method. Sequences of variant specific primers of the molecular marker are as follows: a forward primer MITE-INS-F, and a reverse primer MITE-INS-R; the sequences of non-variant specific primers are as follows: a forward primer WILD-F, and a reverse primer WILD-R. The back cross breeding method utilizing the molecular maker comprises the following steps: carrying out hybridization on a peanut parent of a gene type AABB and a parent of a gene type aabb, then carrying out cross breeding; amplifying an FAD2A gene segment by utilizing one pair of primers in a genome, and selecting a single plant containing a genotype Aa; carrying out PCR (polymerase chain reaction) detection by utilizing a primer, at a site ahFAD2B, of a peanut as a marker, and selecting a single plant containing a genotype Bb; carrying out back crossing on the single plant which is dominant in two markers, namely the single plant containing the genotype AaBb, and the parent AABB until a high-oleic-acid-content single plant which is of a genotype aabb and is consistent with a recurrent parent in other economical characters is selected from later generations, and then carrying out self-crossing and amplifying propagation. The high-oleic-acid-content molecular marker has the advantages of being simple in method, low in cost and stable in result; when the high-oleic-acid-content molecular marker is used for carrying out the back cross breeding, the accuracy of character selection can be obviously improved, the breeding cost is reduced, and the breeding efficiency is improved.

Description

technical field [0001] The invention belongs to the field of agricultural bioengineering, and in particular relates to a molecular marker for high oleic acid, a backcross breeding method for assisting selection by using the marker and its application. Background technique [0002] Cultivated peanuts ( Arachis hypogaea ), also known as groundnut, Papilionaceae, is an annual herb. Peanuts are originally produced in South America, and their planting areas are mainly distributed in Asia, Africa and America, of which Asia accounts for about 60%, Africa accounts for about 30%, and America accounts for about 5.5%. my country is the world's largest peanut producer, consumer and exporter, and peanuts are grown in all provinces, municipalities and autonomous regions. Peanut is an important oil crop, and its fatty acid composition has a great influence on the nutritional quality and storage quality of peanut. Oleic acid and linoleic acid in peanut oil account for about 80% of the to...

AI Technical Summary

Problems solved by technology

[0005] In order to use high oleic acid mutants as hybrid parents for molecular marker-assisted selection (MAS), Barkley et al. (2010) developed real-time quantitative PCR at the F435 mutation site method for high oleic acid genotyping; Chen et al. (2010) developed a general PCR method specific for the F435 mutation site; Chu et al. These methods have corresponding shortcomings, such as high cost of fluorescent labeling, unstable results, cumbersome methods, and high cost of enzymes.

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