A snp marker affecting lambing traits of Hu sheep and its application

Abstract

The invention belongs to the field of molecular biotechnology and molecular marker technology, in particular to the application of a sheep SNP molecular marker in the study of lambing traits and sheep breeding. The goat SNP molecular marker site is as shown in SEQ ID NO.1, and the nucleic acid single base mutation at position 343 of the sequence fragment is named: T-87C. The SNP molecular marker corresponds to the T>C mutation at ‑87bp of the coding sequence of the BMPR‑IB gene (accession number: NC_019463.2) on the sheep chromosome 6 that has been published on GeneBank. By optimizing the dominant allele of the SNP molecular marker, the invention can increase the genetic progress of lambing traits of Hu sheep, shorten the breeding time of reproductive traits of Hu sheep, thereby reducing breeding costs and effectively improving the economic benefits of the mutton sheep breeding industry.

Description

technical field [0001] The invention belongs to the field of molecular biotechnology and molecular marker technology, and in particular relates to the application of a Hu sheep SNP molecular marker in the study of sheep lambing traits and sheep breeding. Background technique [0002] Lamb size is the most important reproductive trait of sheep. It is affected by factors such as genes and environment, and its economic benefits are very significant. It is the main factor affecting the development of mutton sheep industry. Therefore, lambing traits are the focus of research on genetic improvement of breeding sheep. It is difficult to quickly improve the reproductive performance of mutton sheep by conventional breeding methods, but marker assisted selection (MAS) breaks this technical barrier and can eliminate the selection of phenotypic traits by factors such as age, sex, and environment. interference, thus making up for the lack of conventional phenotype selection (Wang Baichua...

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Problems solved by technology

Patent CN104099330A discloses that the allelic mutation occurs at position 713 of the LHβ gene fragment of sheep (breeds include Dorper and Hu sheep), which affects the number of lambs born in Mianyang

[0005] However, there are still some problems in molecular breeding at present, mainly including: the limitation of marker genetic map; the polymorphism frequency of molecular markers linked to breeding target traits is low; The degree of automation is low, and the required equipment and reagents are expensive

However, in gene mapping, due to the limitations of current mapping materials, there is often the problem of inaccurate positioning, and in the technology of QTL mapping of quantitative trait loci, not all target QTLs can be detected, and QTL and There are problems such as the interaction between the environment and the masking of QTLs with large effects on QTLs with small effects

In addition, the RFLP labeling method used in this study also has technical defects such as a large amount of DNA required, complicated detection techniques, and difficulty in applying to large-scale breeding practices.

And these factors have increased the difficulty of finding a new SNP molecular marker

[0006] In addition, some existing molecular markers for lambing traits have different genotypes in their alleles, and the difference in the number of lambs is not particularly significant, which is not particularly ideal for the improvement of the number of lambs

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