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Molecular marker on pig chromosome No.7 related to duroc pig daily weight gain property and application

A molecular marker and chromosomal technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., which can solve the problem that QTL cannot be accurately located at one time, multiple traits cannot be analyzed at one time, and QTL mapping resolution is poor. and other problems to achieve the effect of increasing core competitiveness, reducing feeding costs, and speeding up the breeding process

Active Publication Date: 2019-08-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the candidate gene method has the advantages of simple operation and low total cost, it can only target genes with known biological functions, and cannot find QTLs with unknown biological functions; compared with the candidate gene method, QTL mapping is a more precise mapping method , but the resolution of QTL mapping is poor, and QTLs related to traits cannot be accurately positioned at one time, nor can multiple traits be analyzed at one time, the cycle is long and the efficiency is low; GWAS is considered as a new method for high-resolution genetic analysis

Method used

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  • Molecular marker on pig chromosome No.7 related to duroc pig daily weight gain property and application
  • Molecular marker on pig chromosome No.7 related to duroc pig daily weight gain property and application
  • Molecular marker on pig chromosome No.7 related to duroc pig daily weight gain property and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 concrete explanation obtains the assay process that affects daily weight gain in the present invention

[0035] The average daily gain of live pigs from 30 kg to 100 kg body weight was measured by the Osborne FIRE Pig Performance Testing System (Kansas, NE, USA). The experimental pig group used in the present invention is 2121 purebred plus-line Duroc of Guangdong Wen's Food Group Co., Ltd. Breeding Pig Branch Company, which is the core group of the Breeding Pig Branch Company, and the group pedigree records are detailed. In this experiment, the Canadian Duroc breed pigs in this resource group were selected. The pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

Embodiment 2

[0036] Embodiment 2 specifically explains the inventive process of obtaining the gene marker in the present invention

[0037] (1) The method of extracting DNA from the ear-like tissues of the Canadian Duroc pig was extracted from the whole genome DNA according to the marked phenol-chloroform method. Using Nanodrop-ND1000 spectrophotometer to detect the quality and concentration of the DNA of the purebred plus line Duroc population. The ratio of A260 / 280 is 1.8-2.0, and the ratio of A260 / 230 is 1.7-1.9. Finally, the qualified DNA samples were uniformly diluted to 50 ng / μl.

[0038] (2) 50K SNP genotype detection of the whole pig genome: GeneSeek Genomic Profiler Porcine50KSNP typing platform, using Illumina Infinium instructions and standard procedures for chip hybridization and result scanning. Finally, the genotype data were read by GenomeStudio software. The quality control of the obtained genotype data was performed with PLINK v1.07, and the detection rate was -6 Indivi...

Embodiment 3

[0044] Embodiment 3 specifically explains the inventive process of inventing and detecting SNP markers

[0045] (1) The target segment containing the SNP site significantly related to the average daily weight gain of the Canadian Duroc 30-100 kg is a 214bp nucleotide sequence in chromosome 7, and the upstream and downstream primers for sequence amplification are primer-F and primer-R, its nucleic acid sequence is as follows:

[0046] Upstream primer primer-F: 5'-CCAGGAGAACTGGGTCACAT-3';

[0047] Downstream primer primer-R: 5'-TGGCTCATTCAGTGCAGAAC-3';

[0048] (2) PCR amplification system and condition setting

[0049] Configure a 10 μL system, including 1.0 μL DNA sample, 0.3 μL upstream primer, 0.3 μL downstream primer, 5.0 μL PCR mix, ddH 2 O3.4 μL, PCR conditions were pre-denaturation at 95°C for 5 min, denaturation at 95°C for 30 s, annealing at 64°C for 30 s, extension at 72°C for 30 s, a total of 35 cycles, and a final extension at 72°C for 5 min.

[0050] (3) DNA se...

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Abstract

The invention belongs to the field of molecular biological techniques and molecular marker techniques, and particularly relates to a molecular marker on a pig chromosome No.7 related to the duroc pigdaily weight gain property and application of the molecular marker. An SNP site of the molecular marker on the pig chromosome No.7 related to the duroc pig daily weight gain property corresponds to the A>C mutation at the 27045611th-bp site on the international pig reference genome 11.1 version chromosome No.7, the molecular marker is obtained through whole-genome correlation analysis, the averagedaily weight gain property of 30-100 kg of a pig is obviously affected by the molecular marker. The invention further provides a primer pair for authenticating the molecular marker. By means of the molecular marker and the primer pair, the efficient and accurate molecular marker assisted breeding technology can be established; by applying the technology to the pig daily weight gain property genetic improvement, the daily weight gain of the pig is improved, enterprise benefits are improved, and core competitiveness is improved.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and in particular relates to a molecular marker located on pig No. 7 chromosome and related to the trait of daily weight gain of Duroc pigs and an application thereof. Background technique [0002] Pork is one of the main meats consumed by humans, and it is particularly important to improve the growth performance of pigs to meet people's demand for pork. Average daily gain refers to the average daily weight gain of pigs during the fattening period (30-100kg), which is one of the important indicators to measure the growth performance of pigs and one of the main breeding goals in pig breeding. Breeders have been committed to improving the average daily gain of pigs, and after long-term breeding, the average daily gain has been improved to a certain extent. However, daily gain is a complex quantitative trait regulated by multiple genes, and conventional breedi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴珍芳杨杰吴杰周身娉郑恩琴徐铮蔡更元
Owner SOUTH CHINA AGRI UNIV
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