Bio-enzyme and microorganism containing compound feed additive and preparation process thereof

A compound feed and bio-enzyme technology, applied in animal feed, animal feed, application, etc., can solve the problems of easy reduction of Bacillus subtilis viable bacteria count, reduced effect of additives, weakened digestion effect of feed additives, etc.

Inactive Publication Date: 2012-07-25
LIAONING SHENNONG BIOLOGICAL ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The activity of the enzyme contained in the beneficial bacteria of this invention is small, and the contained microorganisms also contain Lactobacillus acidophilus (Lactobacillus acidophilus) belonging to anaerobic bacteria. Similarly, in the production process of feed additives containing microorganisms and biological enzymes , especially the anaerobic culture process will lead to an increase in cost, and the actual feeding of Lactobacillus acidophilus will also reduce the effect of additives due to its strict living conditions, thereby affecting the effect of feeding; Lactobacillus acidophilus is a kind of live bacteria preparation strain. Because Lactobacillus acidophilus reproduces slowly, its seed cultivation time is as long as 92 to 96 hours. In the drying process of live bacteria preparation, whey powder is used as a protective agent, and the cost is relatively low. High; Bacillus subtilis is used as the enzyme preparation strain, and the fermentation liquid is spray-dried during the preparation process, which not only has high cost, but also easily reduces the number of viable Bacillus subtilis bacteria, which is not enough to meet the needs of animals for digestion and absorption of feed. The digestive effect of feed additives entering the animal body is weakened, so that it cannot effectively balance the beneficial bacteria in the animal's digestive tract and improve the digestion and utilization rate of feed

Method used

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  • Bio-enzyme and microorganism containing compound feed additive and preparation process thereof
  • Bio-enzyme and microorganism containing compound feed additive and preparation process thereof
  • Bio-enzyme and microorganism containing compound feed additive and preparation process thereof

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specific Embodiment approach 1

[0072] A compound feed additive containing biological enzymes and microorganisms proposed by the present invention is composed of beneficial bacteria components and biological enzyme components, and the beneficial bacteria components contain Bacillus subtilis, Saccharomyces cerevisiae, Trichoderma reesei and Aspergillus niger , the biological enzyme component contains cellulase, α-amylase and protease, and the compound feed additive containing biological enzyme and microorganism is made of liquid seeds activated by Bacillus subtilis and Saccharomyces cerevisiae by liquid fermentation and 40-50 The living bacteria preparation prepared by drying at low temperature at ℃ and the enzyme preparation prepared by solid fermentation and drying of liquid seeds activated by Trichoderma reesei and Aspergillus niger are compounded at a ratio of 1:3; this product contains The number of viable microorganisms >8×10 9 cfu, the final cellulase activity is the amount of cellulose degraded per gr...

specific Embodiment approach 2

[0088] A kind of preparation technology of the compound feed additive containing biological enzyme and microorganism of the present invention adopts following steps:

[0089] 1. Preparation of viable bacterial preparations

[0090] 1.1. Strain preparation

[0091] The present invention selects Bacillus subtilis ( Bacillus subtilis ) and Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) as live bacteria preparation strains;

[0092] 1.2. Fermentation medium preparation

[0093] The present invention selects crushed corn and soybean meal, the ratio of corn and soybean meal is 10:1, adds warm water at 40-50°C, the ratio of material to water is 1:2.5-1:3.5, mixes well and heats to 75-85 °C, the time is 40-50 minutes; cook at 130-135 °C, 0.10-0.15 MPa for 80-90 minutes, cool to 60-70 °C, add high-temperature liquefying enzyme and glucoamylase for 3-4 hours to obtain glucose value (DE value) above 65% and 12-14°BX corn and soybean meal enzymatic hydrolysis saccharificatio...

specific Embodiment approach 3

[0142] In the fermentor culture step in the fermented liquid preparation step prepared by the live bacterial agent, the fermentor culture that is carried out separately can be combined into a mixed fermenter culture of Bacillus subtilis and Saccharomyces cerevisiae, that is to say, the liquid seeds of Bacillus subtilis and Saccharomyces cerevisiae are respectively pressed 3% to 5% are co-inoculated into corn and soybean meal enzymatic saccharification liquid for mixed culture, 10L automatic seed fermentation tank is filled with 5L seed fermentation medium, and cultivated at 28-32°C, 180-200rpm (rev / min) for 20 hours , other steps are with embodiment 2. Adopting this implementation mode can save materials and energy consumption in fermentation tanks and production.

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Abstract

The invention discloses a bio-enzyme and microorganism containing compound feed additive and a preparation process thereof, which solve the problems of high production cost and less viable count of beneficial microorganisms in the prior art. The bio-enzyme and microorganism containing compound feed additive is characterized in that the additive is prepared by compounding a viable organism preparation (prepared by carrying out liquid fermentation and low-temperature drying on bacillus subtilis and brewer's yeast) with an enzyme preparation (prepared by carrying out solid fermentation and drying on trichoderma reesei and Aspergillus niger); and the viable count of beneficial microorganisms in each gram of the product is greater than 8*10<9> cfu/g, the cellulase activity (refer to the quantity of cellulose degraded by each gram of the product per minute) is greater than 500 mg, the alpha-amylase activity (refer to the quantity of starch hydrolyzed by each gram of the product per minute) is greater than 3000 mg, and the protease activity (refer to the mount of an amino acid generated by caseins hydrolyzed by each gram of the product per minute) is greater than 400 mg. The bio-enzyme and microorganism containing compound feed additive disclosed by the invention has the advantages that: the feed utilization rate of livestock can be improved in a short term, the intestinal digestive flora of livestock is improved and balanced, the breeding cost is reduced, and the feed conversion rate and the animal production performance are improved; and meanwhile, the additive is simple in preparation process, low in production cost, and suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation and feed additives, in particular to a compound feed additive containing biological enzymes and microorganisms and a preparation process thereof. Background technique [0002] With the development and improvement of microbiology and microecology, microbial feed additives are more and more widely used in the feed industry. Microbial feed additive is a preparation that utilizes active microorganisms, their metabolites, and growth-regulating substances that are beneficial to the host. It is non-toxic, non-residual, and non-polluting, and is of great significance to environmental protection and food safety. At present, the research and development of microbial feed additives at home and abroad are mainly concentrated in three categories: one is live bacterial preparations represented by Bacillus subtilis and yeast cultures, which contain active substances and live bacteria that regulate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/165
Inventor 宋超
Owner LIAONING SHENNONG BIOLOGICAL ENG
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