SNP molecular marker relevant to growth traits of large white pig and application of SNP molecular marker

A technology of growth traits and molecular markers, applied in the determination/testing of microorganisms, recombinant DNA technology, biochemical equipment and methods, etc., to achieve the effects of large economic application value and scientific research value, saving production costs, and accelerating genetic progress

Active Publication Date: 2019-03-01
FOSHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the research on DHRS4 gene mainly focuses on mice and human tumor cells, and there are few reports on pig muscle development, and the related molecular pathways and mechanisms in the pathways of pig growth traits still need further research

Method used

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  • SNP molecular marker relevant to growth traits of large white pig and application of SNP molecular marker
  • SNP molecular marker relevant to growth traits of large white pig and application of SNP molecular marker
  • SNP molecular marker relevant to growth traits of large white pig and application of SNP molecular marker

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Experimental program
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Embodiment

[0024] 1. Collection of experimental samples

[0025] 247 large white pigs from a certain pig farm in Shandong were randomly selected, and the ear tissue samples of the pigs were collected one by one, placed in 75% alcohol and stored at -20°C until DNA was extracted.

[0026] 2. Extraction and detection of porcine genomic DNA

[0027] (1) Cut the pig ear tissue of the sampled pig, each 30 mg, and place it in a 1.5 ml centrifuge tube after cutting into pieces;

[0028] (2) Take a 50ml centrifuge tube, mix the proteinase K with a final concentration of 0.4mg / ml and the lysate, and add 0.5ml of the lysate to the 1.5ml centrifuge tube containing the pig ear tissue for lysis;

[0029] (3) Place the centrifuge tube evenly on a constant temperature shaker (tightly seal the tube cap to avoid liquid extravasation), place it at 55°C overnight, and digest until the tissue sample cannot be observed with the naked eye;

[0030] (4) After the sample is fully cracked, take out the centrifu...

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Abstract

The invention discloses an SNP molecular marker relevant to growth traits of a large white pig and application of the SNP molecular marker. The invention belongs to the molecular marker breeding technical field. The SNP molecular marker is located on the locus rs701332503 of the gene DHRS4 of the large white pig. The nucleotide sequence of the gene DHRS4 in the large white pig is relevant to the growth traits of the large white pig from the 5' end locus rs701332503, that is to say, the SNP molecular marker on the locus rs701332503 of the gene DHRS4 of the large white pig is relevant to the growth traits like the feed conversion ratio of the large white pig and is a new molecular marker, by determining the genotype of the SNP locus of the to-be-tested large white pig, the growth traits likethe feed conversion ratio of the large white pig are subjected to breeding pig sifting, the production cost can be effectively reduced, genetic progress can be accelerated, sifting of large white breeding pigs is better served, the huge market requirement can be met, and the SNP molecular marker also has high economic application value and scientific research value.

Description

technical field [0001] The invention belongs to the technical field of molecular marker breeding, in particular to a SNP molecular marker related to the growth traits of large white pigs and its application. Background technique [0002] Single Nucleotide Polymorphisms (Single Nucleotide Polymorphisms, SNPs) are base variations generated by a single nucleotide in the genome, which are characterized by a large number of distributions and abundant polymorphisms. Another important molecular genetic marker is called the third generation molecular genetic marker. The marker has been widely used in animal and plant marker-assisted breeding, gene mapping, genetic diversity research and other fields. [0003] Animal husbandry occupies a very important position in the development of modern agriculture, and its ratio in agricultural production is usually used as an important indicator to measure the development level of a country and region. Pork is the most important source of meat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 杨言昭陈文坚郭照良朱晓萍
Owner FOSHAN UNIVERSITY
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