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Porcine birth weight trait related SNP marker and use thereof

A marker and locus technology, applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problem of high piglet mortality, increase economic benefits, increase genetic progress, and improve birth weight. Effect

Active Publication Date: 2018-03-30
SOUTH CHINA AGRI UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditionally, the birth weight of piglets has been increased mainly by breeding excellent breeding pigs, strengthening breeding management, and strengthening feeding management of sows during gestation. However, the high mortality rate of piglets caused by low birth weight has been plagued by many breeding work. By

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  • Porcine birth weight trait related SNP marker and use thereof
  • Porcine birth weight trait related SNP marker and use thereof
  • Porcine birth weight trait related SNP marker and use thereof

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Embodiment 1

[0029] Embodiment 1: obtain the inventive process of nascent weight performance in the present invention

[0030] Phenotype data collection: After the piglets were born, the body weight of each piglet at 2 hours after birth was measured by a standard weight scale as the phenotype value of the newborn weight trait.

Embodiment 2

[0031] Embodiment 2: obtain the invention process of gene marker in the present invention

[0032] (1) The method of extracting DNA from large white pig ear-like tissue refers to the phenol-chloroform method to extract whole-genome DNA. Use Nanodrop-ND1000 spectrophotometer to detect the quality and concentration of the DNA of the purebred Great White population. The ratio of A260 / 280 is 1.8-2.0, and the ratio of A260 / 230 is 1.7-1.9. Finally, the qualified DNA samples were uniformly diluted to 50 ng / μl.

[0033] (2) 80K SNP genotype detection of the whole pig genome: GeneSeek Genomic Profiler Porcine 80KSNP typing platform, using Illumina Infinium instructions and standard procedures for chip hybridization and result scanning. Finally, the genotype data were read by GenomeStudio software. The quality control of the obtained genotype data was carried out with PLINK v1.07, and the detection rate of elimination was <99.7%, and the allele frequency (mimor allel frequency, MAF) ...

Embodiment 3

[0038]Embodiment 3: Invention process for detecting SNP markers

[0039] (1) Amplification of the target fragment containing the SNP site significantly related to the birth weight performance of Dabai. The target fragment is a 297bp nucleotide sequence in chromosome 12, and the upstream and downstream primers for sequence amplification are:

[0040] Upstream primer 5'-CAAGCCGACCTTTCCTCCTT-3'

[0041] Downstream primer 5'-CGGGTTTTGTGTCAGCGTTC-3'

[0042] (2) PCR amplification system and condition setting

[0043] Configure 20ul system, including DNA sample 3.5ul, upstream primer 0.6ul, downstream primer 0.6ul, PCR mix10ul,ddH 2 O 5.3ul, PCR conditions were 95°C pre-denaturation for 2min, 95°C denaturation for 10s, 56.5°C annealing for 15s, 72°C extension for 30s, a total of 36 cycles, and the final extension was 72°C for 7min.

[0044] (3) DNA sequence sequencing identification: Sequence sequencing was carried out in Shenzhen Huada Gene Technology Co., Ltd., and the positive...

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Abstract

The invention provides a porcine birth weight trait related SNP marker. The locus of the SNP marker is the 6083856th nucleotide locus on the chromosome No.12 of international pig reference genomes version 11.1, and the base of the locus is T or C. By optimizing preponderant alleles of SNP, the genetic progress of large white birth weight traits can be increased, and the breeding time of the largewhite birth weight traits can be reduced to effectively improve economic benefits of breeding pig breeding.

Description

technical field [0001] The invention relates to a SNP marker related to pig birth weight traits and its use. Background technique [0002] The birth weight of piglets refers to the weight of piglets at birth, which is closely and positively correlated with the weaning survival rate, weaning weight, and slaughter weight. Studies have shown that for every 0.1kg increase in the birth weight of piglets, the weaning weight can be increased by 1kg, and the slaughter weight can be increased by 10kg, that is, at a ratio of 1:10:100. Therefore, the birth weight of piglets can affect the growth and production performance of pigs throughout their lives, and is an important indicator for evaluating pig growth performance. [0003] Piglet birth weight is a typical quantitative trait controlled by polygenes. There are many factors that affect the birth weight of piglets, including the breed of sow, age at first mating, gestational age, body condition, litter size, breed of boar and envi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 吴珍芳杨杰李绍云全建平蔡更元杨明郑恩琴
Owner SOUTH CHINA AGRI UNIV
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