SNP molecular marker influencing wool fiber diameter of alpine merino sheep and application of SNP molecular marker
A technology of molecular markers and merino sheep, applied in the field of genetic engineering, can solve the problems that important genetic resources have not been effectively excavated and utilized, and the molecular marker system of fine-haired wool traits has not been established, so as to shorten the breeding cycle and improve the quality of selection. Accuracy, the effect of speeding up the breeding process
Active Publication Date: 2020-12-08
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology
In the past 50 years, fine-wool sheep genetics and breeding experts at home and abroad have been devoted to the research of candidate genes or molecular markers controlling wool traits, but so far no molecular marker system for fine-wool traits has been established, and a large number of important genetic resources for regulating wool traits are still available. Not being exploited and utilized effectively
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Embodiment 1
[0040] Example 1 Correlation Between Different Genotypes of Alpine Merino Sheep and Wool Fiber Diameter
[0041] 1 material
[0042] 1.1 Sample collection
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Abstract
The invention relates to the technical field of gene engineering, in particular to an SNP molecular marker influencing the wool fiber diameter of alpine merino sheep and application of the SNP molecular marker. The SNP molecular marker influencing the wool fiber diameter of the alpine merino sheep is positioned on the 44288858th nucleotide locus A / G mutation on the seventh chromosome of the international sheep reference genome Oar_v4.0 version. The invention also provides a specific primer pair for identifying the SNP molecular marker and a kit containing the specific primer pair, and the specific primer pair and the kit can be used for early selection of a superfine strain of the alpine merino sheep to improve the seed selection accuracy, shorten the cultivation period and accelerate thebreeding process. The kit has the advantages of simple operation, can shorten the breeding time of excellent traits of the wool fiber diameter of the alpine merino sheep, reduce the breeding cost, shorten the cultivation period and accelerate the cultivation process, and has very high application value.
Description
technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a SNP molecular marker affecting the fiber diameter of Alpine Merino sheep and its application. Background technique [0002] The new breed of alpine Merino sheep is the world's first new breed of wool and meat Merino sheep that can adapt to the alpine cold and drought ecological zone at an altitude of 2400-4070m and the wool fiber diameter is 19.1-21.5μm. Excellent germplasm resources are provided. The wool yield and fiber fineness quality of fine-wool sheep are important indicators to determine its economic value, and the objective inspection of wool quality is getting more and more attention in wool sales, and it is more and more combined with textile performance, breeding and production. Tightness, wool fiber diameter is the most important test index in wool fiber inspection, almost all properties of wool are related to or directly determined by fiber diameter. ...
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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 袁超郭婷婷卢曾奎刘建斌岳耀敬牛春娥杨博辉孙晓萍李建烨
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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