Multisite polymerization effect analytic breeding method for increasing lambing number of Hu sheep

A technology of lambing number and multiple loci, applied in the field of molecular biotechnology and molecular markers, can solve the problems of poor selection effect, long time-consuming, low breeding efficiency, etc., to increase lambing number, reduce breeding time, and speed up breeding process. Effect

Active Publication Date: 2018-12-07
SOUTH CHINA AGRI UNIV
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AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defects and deficiencies of the prior art in genetic selection of litter size traits, to provide a multi-site polymeric molecular marker that affects the litter size traits of Hu sheep, and to improve the litter size traits of Hu sheep. The multi-gene aggregation breeding method, the core technology of this method lies in a molecular marker related to the litter size traits of Hu sheep, in order to overcome the long time-consuming conventional breeding technology, low breeding efficiency and the selection of single locus molecular markers for complex economic traits Insufficient effect

Method used

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  • Multisite polymerization effect analytic breeding method for increasing lambing number of Hu sheep
  • Multisite polymerization effect analytic breeding method for increasing lambing number of Hu sheep
  • Multisite polymerization effect analytic breeding method for increasing lambing number of Hu sheep

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Experimental program
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Effect test

Embodiment 1

[0056] Application of embodiment 1 biological technology

[0057] In the embodiment, 934 Hu sheep ewes with complete lambing records were selected, and 5 mL of jugular vein blood was drawn and anticoagulated, and stored at -20°C for later use. The Hu sheep used in this research all come from the bougainvillea sheep breeding farm of Wenshi Xinwang Sheep Industry Co., Ltd., Yunfu City, Guangdong Province. The farm is raised in high-bed houses throughout the year, and fed rations according to the uniform feeding standard, and free to eat drinking water.

[0058] (1) The extraction of Hu sheep blood genomic DNA was performed with reference to the blood genomic DNA extraction kit (purchased from Guangzhou Ruiyang Biotechnology Co., Ltd.), and detected by 1% agarose gel electrophoresis, without dispersion or tailing phenomenon; An ultraviolet spectrophotometer was used to detect the quality and concentration of the extracted DNA. DNA samples with A260 / 280 ratio of 1.8 to 2.0 and A...

Embodiment 2

[0063] The establishment of embodiment 2 single-point effect analysis model

[0064] (1) Amplification of target fragments containing SNPs loci significantly related to litter size traits of Hu sheep: the target fragments are all partial nucleotide sequences on chromosome 6, and the upstream and downstream primers for sequence amplification are based on reports on GenBank The sequence of the sheep BMPR-IB gene (accession number: NC-019463.2), using the Primer Premier 5.0 software to design primers for the three SNPs of the BMPR-IB gene, amplified the partial sequence of the Hu sheep BMPR-IB gene. The primers were synthesized by Huada Biotechnology Co., Ltd., and the specific sequences are as follows:

[0065] SEQ ID NO.4:

[0066] The upstream primer PCR-F of the T-87C site: 5'-TTTTAGGGCTCCATGTACTCC-3';

[0067] SEQ ID NO.5:

[0068] Downstream primer PCR-R of T-87C site: 5'-TTTCCTCTGGGTGCTGTG-3'.

[0069] SEQ ID NO.6:

[0070] The upstream primer PCR-F of the A746G site: 5...

Embodiment 3 3

[0100] Example 3 Establishment of Three-site Polymerization Effect Analysis Model

[0101] First, the methods described in Examples 1 and 2 were used to detect the T-87C, A746G and G765A polymorphic sites of the BMPR-IB gene, and the single-site effect analysis was carried out with the litter size of Hu sheep. The above results showed that BMPR-IB The three polymorphic sites of the IB gene all had a significant impact on the litter size of Hu sheep. In order to study the interaction between the above three loci and further improve the breeding efficiency of the litter size traits of Hu sheep, the present invention established a multi-site aggregation effect analysis model for the litter size traits of Hu sheep.

[0102] (1) Three-site aggregation effect analysis model

[0103] A total of 934 Hu sheep were tested, the T-87C / A746G / G765A combined genotype was determined by PCR-RFLP method, and the correlation analysis between the combined genotype and litter size was carried out...

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Abstract

The invention belongs to the technical field of molecular biological and molecular marker technologies and especially relates to a multisite polymerization effect analytic breeding method for increasing lambing number of Hu sheep. BMPR-IB gene serves as a candidate gene that influences the character of the lambing number of Hu sheep, wherein there are three SNPs molecular markers, T-87C site, A746G site and G765A site, in the gene; by performing T-87C / A746G / G765A triple-site polymerization effect analysis to the SNPs molecular markers, it is found that CCGGGA is an optimum combined genotype. By means of the optimization of combined genotype, the genetic progress on the character of lambing number of Hu sheep is accelerated and early-stage selection efficiency of the Hu sheep is increased,thereby effectively increasing the economic benefit of mutton sheep breeding industry.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and in particular relates to a breeding method for analyzing multi-site aggregation effects for improving the litter size of Hu sheep. The invention uses the molecular markers in combination in the study of the litter size traits of Hu sheep and the early breeding work of Hu sheep. Background technique [0002] Reproductive traits are important economic traits. For breeding stock, fecundity is productivity, and reproductive performance is directly related to the economic effect of mutton sheep industry. In traditional breeding methods, the selection of livestock traits mostly relies on the phenotypic selection of livestock. The breeding of large livestock often takes decades, and it is difficult to quickly improve the reproductive performance of livestock groups. Studying the breeding method to improve lambing rate from the genetic nature is a technical pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 柳广斌杨新月刘德武李耀坤孙宝丽
Owner SOUTH CHINA AGRI UNIV
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