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SNP molecular marker related to broiler chicken weight and shin length character, and application of SNP molecular marker

A molecular marker, broiler technology, applied in the field of genetic engineering, can solve the problems of a large number of seed selection, seed production, time-consuming, slow breeding process, etc., achieve great economic application value and scientific research value, save production costs, and speed up genetics. effect of progress

Active Publication Date: 2021-08-06
SOUTH CHINA AGRI UNIV
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Problems solved by technology

But its breeding process is slow and takes a lot of time
The results of breeding are complex and unpredictable, requiring a lot of work in seed selection and seed production

Method used

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  • SNP molecular marker related to broiler chicken weight and shin length character, and application of SNP molecular marker
  • SNP molecular marker related to broiler chicken weight and shin length character, and application of SNP molecular marker
  • SNP molecular marker related to broiler chicken weight and shin length character, and application of SNP molecular marker

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Embodiment

[0023] The broilers to be tested are F2 resource populations of Xinghua chicken and recessive white Roc chicken, among which the recessive white Roc chicken is a fast large broiler chicken, and Xinghua chicken is a broiler breed native to Guangdong, China. The chickens are raised in a flat way and fed corn-soybean meal-based feed that meets international formula standards. Then the body weight and shank length of the broiler chickens were measured at each week from 1 to 17 weeks of age. Blood was collected from the subwing vein before slaughter, and the collected blood was stored in a centrifuge tube containing 2% EDTA anticoagulant for blood DNA extraction.

[0024] 1. Extract the blood DNA of broiler chickens to be tested from the full-sib family of Xinghua chicken × recessive white Roc chicken F2 generation resource group

[0025] Genomic DNA of all individuals based on Plant Mini Kit (Qiagen, Hilden, CA; Cat#69104) was used in the kit instructions to extract blood DNA, ...

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Abstract

The invention discloses an SNP molecular marker related to a broiler chicken weight and shin length character, and application of the SNP molecular marker. The SNP molecular marker is positioned on the 135355388th nucleotide of the No.1 chromosome of a galGal6 version chicken genome, and corresponds to a mutation site g. 880C>T in a chicken TMEM182 gene sequence. The genotypes of the SNP molecular marker are CC, TCA and TT, wherein the weight of the CC genotype individual is greater than the weight of the TC and TT genotype individuals, and the shin length of the CC genotype individual is greater than the shin length of the TC and TT genotype individuals. The SNP molecular marker disclosed by the invention is characterized in that the mutation site g. 880C>T in a chicken TMEM182 gene intron 2 sequence is related to the weight and shin length character, the SNP molecular marker is a new molecular marker, early selection is carried out on the broiler chicken shin length character through determination of the genotype of the chicken SNP locus, production cost can be saved, and genetic progress is quickened.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a SNP molecular marker related to the shank length trait of broiler chickens and an application thereof. Background technique [0002] The consumer market largely determines the direction of chicken breeding. my country's local broiler breeds are rich in resources, and most of the local broiler breeds have excellent traits such as strong stress resistance and weak nesting ability. With the improvement of living standards and the change of dietary concepts, people have higher requirements for the meat quality of broilers. Due to their excellent meat quality and unique flavor, local breeds are deeply loved by consumers and have a large market demand. However, compared with foreign fast and large broilers, they have defects such as slow growth and low feed consumption ratio, which seriously restricts Chinese local breeds Large-scale production and industrial applicatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 罗文林泽桐张细权聂庆华
Owner SOUTH CHINA AGRI UNIV
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