SNP molecular marker related to egg weight and application of SNP molecular marker

A technology of molecular markers and egg weight, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of egg weight identification and large QTL confidence interval, and achieve accelerated genetic progress and large economic applications Value and scientific research value, the effect of saving production costs

Inactive Publication Date: 2016-02-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the reported QTLs related to egg weight have relatively large confidence intervals, and it is difficult to identify the cause of mutations affecting egg weight through QTL maps

Method used

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  • SNP molecular marker related to egg weight and application of SNP molecular marker
  • SNP molecular marker related to egg weight and application of SNP molecular marker
  • SNP molecular marker related to egg weight and application of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Confirmation of the correlation between rs314058619 site SNP and egg weight

[0031] Experimental materials: 1534 F2 individuals of the cross between Bai Laihang and Dongxiang green-shell layer hens of Jiangsu Institute of Poultry Science.

[0032] 1. Determination of egg weight

[0033] The egg weight of each individual was measured at first egg weight, egg weight at 32 weeks, egg weight at 36 weeks, egg weight at 40 weeks, egg weight at 44 weeks, egg weight at 48 weeks, egg weight at 52 weeks, and egg weight at 56 weeks. Egg weight at 60 weeks old.

[0034] 2. Detection of SNP

[0035] 1. Genomic DNA extraction

[0036] Chicken wing venous blood collection, ACD anticoagulant (1.32% (m / v) sodium citrate, 0.48% (m / v) citric acid, 1.47% (m / v) glucose) anticoagulant after lysis, protease (purchased from Sangong Bioengineering (Shanghai) Co., Ltd.) digested, extracted DNA by phenol imitation method, and dissolved in sterilized double distilled water.

[0037...

Embodiment 2

[0056] Example 2 Correlation analysis between rs314058619T / G genotype and egg weight of F2 generation individuals

[0057] 1. Experimental materials: 1534 F2 individuals of the hybridization of Bailaihang laying hens and Dongxiang green-shell laying hens of Jiangsu Institute of Poultry Science.

[0058] The egg weight of each individual was measured at first egg weight, egg weight at 32 weeks, egg weight at 36 weeks, egg weight at 40 weeks, egg weight at 44 weeks, egg weight at 48 weeks, egg weight at 52 weeks, and egg weight at 56 weeks. Egg weight at 60 weeks old.

[0059] 2. Detection of SNP

[0060] 1. Genomic DNA extraction

[0061] Blood was collected from chicken wings, anticoagulated with ACD anticoagulant, lysed and digested with protease, DNA was extracted by phenol imitation method, and dissolved in sterilized double distilled water.

[0062] 2. Genotype determination

[0063] Genomic DNA was taken, and the genotype of each individual was detected by using 600KA...

Embodiment 3

[0080] Example 3 Application of rs314058619 site SNP

[0081] 1. Experimental materials: Beijing Huadu Yukou Poultry Industry Co., Ltd. 1,065 Luodao red pure-line laying hens were measured for each individual's initial egg weight, 28-week-old egg weight, 36-week-old egg weight, and 56-week-old egg weight. Age egg weight and 66-week-old egg weight.

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Abstract

The invention provides an SNP molecular marker related to egg weight and application of the SNP molecular marker. The SNP molecular marker corresponds to 169, 930, 974th deoxynucleotide of a Number one chromosome positive-sense strand of chicken reference genome Gallus_gallus-4.0 version sequence information issued in NCBI, wherein the nucleotide is T or G. An SNP locus is never reported before, thereby being a newly-found genetic molecular marker. The SNP molecular marker can be applied to early-stage selection of egg weight at different week ages and assisting of chicken breeding, lowering of production cost and accelerating of genetic progress can be facilitated, and the SNP molecular marker has quite high economic application value and scientific research value.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a SNP molecular marker related to egg weight of eggs and an application thereof. Background technique [0002] In poultry production, egg weight is an important economic trait, and selecting the egg weight that meets market demand is the main goal and research purpose of poultry breeding. Since consumers in different countries and regions have different preferences for egg weight, egg weight will directly affect the sales and price of eggs in the market, so egg weight has important economic value. For breeding, egg weight also affects chick quality, including chick birth weight, fitness and performance. [0003] For the same hen, the age factor has a significant effect on the egg weight, and the egg weight increases with the age of the hen throughout the laying period. Excessive egg weight in the late laying period will affect egg packaging and sales. Theref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 杨宁易国强李光奇
Owner CHINA AGRI UNIV
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