Molecular marker capable of affecting abdominal fat percentage of chicken, and application of molecular marker

Abstract

The invention discloses a molecular marker capable of affecting the abdominal fat percentage of a chicken, and application of the molecular marker, particularly relates to application of a chicken ALDH1A3 gene 3' noncoding region sequence g.941 T>C molecular marker as a detection site for authenticating the abdominal fat percentage of the chicken, and is characterized in that the abdominal fat percentage of a CC genotype individual is obviously greater than the abdominal fat percentage of a TT genotype individual and the abdominal fat percentage of a TC genotype individual. When the molecularmarker disclosed by the invention used for carrying out marker assisted selection, the abdominal fat percentage obtained after the chicken is slaughtered can be greatly lowered, a feed use ratio of achicken flock is improved, and breeding cost is lowered. Through the molecular marker combination and a detection primer, an efficient and accurate molecular marker assisted breeding technology can beestablished, then, the efficient and accurate molecular marker assisted breeding technology is applied to abdominal fat percentage character genetic improvement after a breeding chicken is slaughtered, the abdominal fat percentage after the chicken is slaughtered is lowered, a feed use ratio of the chicken flock is improved, the sales of enterprises can be improved, and core competitiveness is increased.

Description

technical field

[0001] The invention relates to the technical field of molecular breeding, and more particularly relates to a molecular marker affecting the abdominal fat rate of chickens and an application thereof. Background technique

[0002] Chicken is the main edible poultry in our country. Chicken meat is tender and delicious. Because of its light taste, it can be used in various dishes all over the country. Phospholipids in chicken play an important role in human growth and development, and are one of the important sources of fat and phospholipids in Chinese dietary structure. At the same time, chicken has a good therapeutic effect on malnutrition, chills, fatigue, irregular menstruation, anemia, and weakness. As the trading market of live poultry is constantly being questioned, chilled chicken is gradually accepted by consumers. Consumers largely determine the breeding direction and sales methods of chickens. For example, in southern China, whole chickens are mainl...

Examples

Embodiment 1

[0035] Example 1 Primer Design

[0036] 1. Experimental method

[0037] Find the sequence of the 3' non-coding region of the ALDH1A3 gene from https: / / www.ncbi.nlm.nih.gov / , and design a primer pair. The primer pair information is shown in Table 1 (primer sequence 5'→3'). The sequence of the primer pair was sent to Guangzhou Sangon Biotech Co., Ltd. for synthesis, and the primer pair was used to perform PCR on tissue DNA to test the specificity of the primers.

[0038] Table 1 Primer information for SNP screening of ALDH1A3

[0039]

[0040] 2. Experimental results

[0041] The results are attached figure 1 shown. Use this primer pair to carry out PCR on blood DNA, as attached figure 1 As shown, the primers are specific.

Embodiment 2

[0042] Example 2 DNA pool sequencing

[0043] 1. Experimental method

[0044] The sample chickens were selected from the 120-day-old Nanhai jute chicken No. 1 pure line of Jiangfeng Chicken Farm. All chickens were hatched in the same batch, raised in a flat rearing manner, and fed corn-soybean meal-based feed that complied with international formula standards. Use a 2ml syringe to collect blood from the subwing vein, collect the blood and store it in a centrifuge tube containing 20% ​​EDTA for blood DNA extraction.

[0045] Genomic DNA of all individuals based on Plant Mini Kit (Qiagen, Hilden, CA; Cat#69104) was used in the kit instructions to extract blood DNA, measure the quality and concentration, dilute to 50 ng / μL, and store at 4°C for later use.

[0046] 30 DNA samples were randomly selected from the total extracted DNA samples to construct a mixed pool, and every three samples (about 0.33 μL of each sample) were mixed into a mixed sample, a total of 10 samples. The...

Embodiment 3

[0049]Example 3 Determination of the genotype of the ALDH1A3 mutation site g.941T>C

[0050] 1. Experimental method

[0051] Before slaughtering, all chicken samples were tested for indicators, in accordance with the Agricultural Industry Standard of the People's Republic of China "Poultry Production Performance Terminology and Measuring and Statistical Methods" (NT / T823-2004). Among them, the subcutaneous fat thickness and intermuscular fat width were measured with a vernier caliper, and the shin circumference and shin length were measured with a soft ruler. After the chickens were bled, scalded and plucked in a unified line in the slaughterhouse, they were gently wiped dry with paper towels, and the cloaca, shin (cuticle removal), Determination of the yellowness value of the shoulders, buttocks, chest, belly and abdominal fat.

[0052] A database was established with Excel, and individuals with incomplete trait data records and outliers were deleted during the initial scre...

technical field

[0001] The invention relates to the technical field of molecular breeding, and more particularly relates to a molecular marker affecting the abdominal fat rate of chickens and an application thereof. Background technique

[0002] Chicken is the main edible poultry in our country. Chicken meat is tender and delicious. Because of its light taste, it can be used in various dishes all over the country. Phospholipids in chicken play an important role in human growth and development, and are one of the important sources of fat and phospholipids in Chinese dietary structure. At the same time, chicken has a good therapeutic effect on malnutrition, chills, fatigue, irregular menstruation, anemia, and weakness. As the trading market of live poultry is constantly being questioned, chilled chicken is gradually accepted by consumers. Consumers largely determine the breeding direction and sales methods of chickens. For example, in southern China, whole chickens are mainl...

Examples

Embodiment 1

[0035] Example 1 Primer Design

[0036] 1. Experimental method

[0037] Find the sequence of the 3' non-coding region of the ALDH1A3 gene from https: / / www.ncbi.nlm.nih.gov / , and design a primer pair. The primer pair information is shown in Table 1 (primer sequence 5'→3'). The sequence of the primer pair was sent to Guangzhou Sangon Biotech Co., Ltd. for synthesis, and the primer pair was used to perform PCR on tissue DNA to test the specificity of the primers.

[0038] Table 1 Primer information for SNP screening of ALDH1A3

[0039]

[0040] 2. Experimental results

[0041] The results are attached figure 1 shown. Use this primer pair to carry out PCR on blood DNA, as attached figure 1 As shown, the primers are specific.

Embodiment 2

[0042] Example 2 DNA pool sequencing

[0043] 1. Experimental method

[0044] The sample chickens were selected from the 120-day-old Nanhai jute chicken No. 1 pure line of Jiangfeng Chicken Farm. All chickens were hatched in the same batch, raised in a flat rearing manner, and fed corn-soybean meal-based feed that complied with international formula standards. Use a 2ml syringe to collect blood from the subwing vein, collect the blood and store it in a centrifuge tube containing 20% ​​EDTA for blood DNA extraction.

[0045] Genomic DNA of all individuals based on Plant Mini Kit (Qiagen, Hilden, CA; Cat#69104) was used in the kit instructions to extract blood DNA, measure the quality and concentration, dilute to 50 ng / μL, and store at 4°C for later use.

[0046] 30 DNA samples were randomly selected from the total extracted DNA samples to construct a mixed pool, and every three samples (about 0.33 μL of each sample) were mixed into a mixed sample, a total of 10 samples. The...

Embodiment 3

[0049]Example 3 Determination of the genotype of the ALDH1A3 mutation site g.941T>C

[0050] 1. Experimental method

[0051] Before slaughtering, all chicken samples were tested for indicators, in accordance with the Agricultural Industry Standard of the People's Republic of China "Poultry Production Performance Terminology and Measuring and Statistical Methods" (NT / T823-2004). Among them, the subcutaneous fat thickness and intermuscular fat width were measured with a vernier caliper, and the shin circumference and shin length were measured with a soft ruler. After the chickens were bled, scalded and plucked in a unified line in the slaughterhouse, they were gently wiped dry with paper towels, and the cloaca, shin (cuticle removal), Determination of the yellowness value of the shoulders, buttocks, chest, belly and abdominal fat.

[0052] A database was established with Excel, and individuals with incomplete trait data records and outliers were deleted during the initial scre...

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