Method for detecting plastic content of marine organism

A technology of marine organisms and plastics, applied in the field of detection of substance content in organisms, to achieve the effects of easy preparation, strong practicability, and accurate experimental detection

Active Publication Date: 2017-05-10
DALIAN OCEAN UNIV
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  • Method for detecting plastic content of marine organism
  • Method for detecting plastic content of marine organism
  • Method for detecting plastic content of marine organism

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[0019] The specific embodiment of the present invention will be described below in conjunction with accompanying drawing: Figure 1 to Figure 3 Shown: a method for detecting plastic content in marine organisms, which is operated according to the following steps: firstly, take the muscle tissue of the marine organisms to be tested, or take the digestive tract tissue from the esophagus to the anal sphincter of the marine organisms on the north side, which is called After weighing, record the quality of the biological tissue, and dry it in an oven at a constant temperature of 60°C, and crush the biological tissue after drying to constant weight in a quartz mortar, and ensure that the particle size range is 5-10mm after crushing. Weigh 5-10g of the pulverized biological tissue sample into a 500ml glass beaker, add 25-50ml of concentrated nitric acid (concentration: 65% nitric acid), boil and digest on the electric heating plate for 30min, the heating temperature range is 90-100℃, ...

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Abstract

The invention discloses a method for detecting the plastic content of a marine organism. The method is characterized by including the steps: drying, crushing and digesting marine organism digestive tracts or muscular tissues; performing density gradient centrifugation for digestion solution; performing microscopic examination for micro-plastics in obtained centrifugal liquid by a fluorescence microscope and a Fourier transform infrared microscope; counting the number of the micro-plastics. The method is simple to operate, high in accuracy and application safety and applicable to marine organism micro-plastic pollution detection.

Description

technical field [0001] The invention relates to a method for detecting the content of substances in organisms, in particular to a method for detecting the content of plastics in marine organisms. Background technique [0002] Plastics and their products are widely used in industry, agriculture and daily life. The world uses no less than 240 million tons of plastics every year. Plastic residues in the environment can migrate long distances through external forces such as wind, rivers, and ocean currents, polluting remote corners of the earth. It has been found from previous reports that there is currently a relatively high degree of plastic debris pollution on the surface and deep sea of ​​the North Atlantic and Pacific Oceans; at the same time, studies have also shown that the pollution of plastic debris in the coastal zone of the South China Sea is also very common. Due to the effects of solar radiation (photodegradation, embrittlement) or waves, these fragments are often ...

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Application Information

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IPC IPC(8): G01N21/64G01N21/3563
CPCG01N21/3563G01N21/64G01N2021/3595
Inventor 魏海峰刘长发刘海映陈明鑫陈明锐
Owner DALIAN OCEAN UNIV
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