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Tissue culture seedling growing process for trichosanthes

A technology of hanging melons and melon pieces, which is applied in the field of plant tissue culture, can solve the problems of low reproduction efficiency, high cost of seedling cultivation, low reproduction rate, etc., and achieve the effects of high-quality and efficient production scale, short reproduction cycle and high reproduction coefficient

Inactive Publication Date: 2007-06-27
江苏春辉生态农林股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seeds are used for sowing and reproduction, and the male plants that do not bear fruit account for about 70% of the seedlings that reproduce naturally. The cost of raising seedlings is high and the reproduction rate is low
Propagation by cutting root tubers, due to the limitation of the number of tubers, the reproduction efficiency is low, and it is labor-intensive and time-consuming, which directly affects the expansion of the planting area and the promotion of fine varieties.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The best way for the present invention to adopt edible hanging melon group cultivation seedling method below:

[0022] Step 1. Selection of explants

[0023] In May, take the buds or vine-like stems that germinate on the root of Diaogua tuber.

[0024] Step 2, processing of explants

[0025] Cut the buds or vine-like stems, remove the leaves and tendrils on the vine-like stems, cut into a leafy stem, first wash with 1% detergent solution for 8 minutes, then rinse with running water for 30 minutes, and dry the surface with clean dry gauze moisture. In the ultra-clean workbench, put the buds or stems into a jar that has been sterilized by high pressure, first soak it with 70% alcohol for 10 seconds, pour out the alcohol solution, rinse it twice with sterile water, and then use 0.1 % mercuric chloride solution for 10 minutes, then rinse with sterile water 4 times, not less than 4 minutes each time, pour off the sterile water washing solution, dry the surface water with ...

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Abstract

The tissue culture seedling growing process for trichosanthes includes the following steps: 1. taking the bud on tuberous root or stalk of trichosanthes as the explant in summer end or initial summer; 2.cutting bud or stem segment; 3. inoculating the bud or stem segment in culture medium I inside culture bottle; 4. secondary culture in culture medium II inside culture bottle; and 5. rooting culture in culture medium III inside culture bottle for 20-30 days to root. The present invention has short propagation period and high propagation coefficient.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for cultivating Diaogua group seedlings for industrialized rapid propagation of Diaogua by means of a basic medium with different components added. It belongs to the technical field of plant tissue culture. Background technique [0002] Diaogua, also known as Trivbosantnes Kirilouii (Trivbosantnes Kirilouii), belongs to the genus Trivbosantnes Kirilouii. It is a perennial herbaceous vine plant. ), according to the records in "Compendium of Materia Medica", Diaogua has the functions of invigorating the stomach, moistening the lungs, resolving phlegm, loosening joints, and smoothing. show. Hanging melon seeds also have anti-inflammatory and anti-cancer effects. According to the "New Anti-Cancer Method Compilation" published by Shanghai Science and Technology Literature Publishing House, it has a significant effect on lung cancer, gastric cancer, and insulin breast cancer....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 吴有光方建民刘唤英
Owner 江苏春辉生态农林股份有限公司
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