Single-end feeding method of agriphila aeneociliella

The technology of the white-browed weed moth and the petri dish is applied in the field of single-head feeding of the white-browed weed moth, which can solve the problems of bacteria, virus infection, dysplasia of test insects, and food contamination, so as to avoid differences in larval development progress and larval nutrition Bad, easy-to-operate effect

Inactive Publication Date: 2016-06-08
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Group feeding with feed usually leads to continuous excrement, contaminating food, causing stunted development of test insects, and even infection and death
And group breeding also has the problem of intraspecific cannibalism
In addition, the cocoons of multi-headed larvae reared in groups are tangled together, making it difficult to separate them, and it is also difficult to keep t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A feeding method for the white-browed weed moth mainly comprises the following steps:

[0025] (1) Egg collection and disinfection: Adults are paired and placed in an egg collection box for adult lepidopteran insect breeding. After the adults start to lay eggs, collect eggs once a day until they no longer lay eggs. After the eggs are collected, sterilize them with 3.5% formaldehyde solution for 1 minute, then take them out with a sterile net bag and soak them in sterile water for 3 times, each time for 5 minutes; after soaking, take them out and use absorbent paper to blot the water on the surface of the eggs.

[0026] (2) Egg hatching larvae and larva rearing: put a piece of sterile water-soaked 1cm 3 Absorbent paper block, place the sterilized eggs in step 1 on the side opposite to the absorbent paper in the petri dish, seal the cling film and place it in a light incubator for cultivation. There are three air holes with a diameter of 2 mm on the cling film. The condi...

Embodiment 2

[0034] A feeding method for the white-browed weed moth mainly comprises the following steps:

[0035] (1) Egg collection and disinfection: Adults are paired and placed in an egg collection box for adult lepidopteran insect breeding. After the adults start to lay eggs, collect eggs once a day until they no longer lay eggs. After the eggs are collected, sterilize them with 3.5% formaldehyde solution for 1 minute, then take them out with a sterile net bag and soak them in sterile water for 3 times, each time for 5 minutes; after soaking, take them out and use absorbent paper to blot the water on the surface of the eggs.

[0036] (2) Egg hatching larvae and larva rearing: put a piece of sterile water-soaked 1cm 3 Absorbent paper block, place the sterilized eggs in step 1 on the side opposite to the absorbent paper in the petri dish, seal the cling film and place it in a light incubator for cultivation. There are three air holes with a diameter of 2 mm on the cling film. The condi...

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PUM

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Abstract

The invention relates to the field of plant protection and an insect breeding technology, in particular to a single-end feeding method of agriphila aeneociliella. The method comprises the steps that a single insect breeding case is adopted to serve as a breeding tool, and entangled cocooning and cannibalism among larvae can be avoided; mass propagation of pathogenic microorganisms such as bacteria and viruses can be effectively inhibited, and bacterium and virus transmission among insects is avoided; degerming and cleaning are conducted conveniently at any time, and a disinfection measure is adopted; timely replacing on feed is facilitated, and group consistency is kept; sterile vermiculite with the moisture of 20% serves as a pupa cell making medium, mature larvae are put on the medium surface, and pupating of the larvae and adult eclosion are facilitated.

Description

technical field [0001] The invention belongs to the field of plant protection and insect breeding technology, and relates to a single-head feeding method of the weed moth. Background technique [0002] Lepidoptera is the second largest order of the order Insecta, and Agriphila aeneociliella (Eversmann, 1844)], which belongs to this family, is a new wheat pest in recent years. Since the outbreak of the insect in 2009, the insect has spread and exploded in Laizhou City. By 2012, it had occurred on more than 6,000 mu in Laizhou, causing great damage to the wheat at the seedling stage. The lack of seedlings and ridges are formed, and more than 50% of the wheat seedlings in serious plots die, or even become extinct. In March 2013, this kind of pest was found to damage wheat in Jincheng, Shanxi. In 2014, the damage occurred in the Gaomi wheat field in Shandong, and in 2015 it spread to Jimo, Qingdao. It causes huge economic losses every year and poses a serious threat to the sa...

Claims

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Application Information

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IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 刘勇迟宝杰刘笑先赵鹏凯
Owner SHANDONG AGRICULTURAL UNIVERSITY
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