Single-end feeding method of agriphila aeneociliella
The technology of the white-browed weed moth and the petri dish is applied in the field of single-head feeding of the white-browed weed moth, which can solve the problems of bacteria, virus infection, dysplasia of test insects, and food contamination, so as to avoid differences in larval development progress and larval nutrition Bad, easy-to-operate effect
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Embodiment 1
[0024] A feeding method for the white-browed weed moth mainly comprises the following steps:
[0025] (1) Egg collection and disinfection: Adults are paired and placed in an egg collection box for adult lepidopteran insect breeding. After the adults start to lay eggs, collect eggs once a day until they no longer lay eggs. After the eggs are collected, sterilize them with 3.5% formaldehyde solution for 1 minute, then take them out with a sterile net bag and soak them in sterile water for 3 times, each time for 5 minutes; after soaking, take them out and use absorbent paper to blot the water on the surface of the eggs.
[0026] (2) Egg hatching larvae and larva rearing: put a piece of sterile water-soaked 1cm 3 Absorbent paper block, place the sterilized eggs in step 1 on the side opposite to the absorbent paper in the petri dish, seal the cling film and place it in a light incubator for cultivation. There are three air holes with a diameter of 2 mm on the cling film. The condi...
Embodiment 2
[0034] A feeding method for the white-browed weed moth mainly comprises the following steps:
[0035] (1) Egg collection and disinfection: Adults are paired and placed in an egg collection box for adult lepidopteran insect breeding. After the adults start to lay eggs, collect eggs once a day until they no longer lay eggs. After the eggs are collected, sterilize them with 3.5% formaldehyde solution for 1 minute, then take them out with a sterile net bag and soak them in sterile water for 3 times, each time for 5 minutes; after soaking, take them out and use absorbent paper to blot the water on the surface of the eggs.
[0036] (2) Egg hatching larvae and larva rearing: put a piece of sterile water-soaked 1cm 3 Absorbent paper block, place the sterilized eggs in step 1 on the side opposite to the absorbent paper in the petri dish, seal the cling film and place it in a light incubator for cultivation. There are three air holes with a diameter of 2 mm on the cling film. The condi...
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