Trichogramma japonicun ashmead artifical breeding production method

A technology for artificial breeding of Trichogramma, applied in animal husbandry and other directions, can solve the problems of difficulty in large-scale production, inability to complete development, difficulty in large-scale, commercialization, etc. High, short reproductive cycle effect

Active Publication Date: 2015-12-16
INST OF ZOOLOGY CHINESE ACAD OF SCI +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Trichogramma rice borer is an important parasitic natural enemy of rice pests such as rice stem borer, rice stem borer and rice leaf roller. The host range of rice borer Trichogramma is relatively narrow. It is still difficult to raise these primary hosts in large quantities. Usually, the eggs of rice moths are used as substitute hosts to breed Trichogramma oryzae. Trichogramma rice borer has been known for many years, but it is difficult to form large-scale and commercialized, which limits the production of organic rice
Trichogramma rice borer can parasitize the eggs of corn borer and armyworm, but the eggs of these two insects are in the form of lumps, which are not easy to remove from the egg-laying utensils to make egg cards, which limits the application; the life cycle of diamondback moth is short, and the reproduction Ligao can quickly expand the population in a short period of time and obtain a large number of eggs, but the eggs are small, resulting in a decline in the quality of the bred Trichogramma rice borer. Similarly, diamondback moth eggs are not easy to make egg cards and form standardization; Spodoptera litura The amount of eggs laid is large, and it is easy to make a standard egg card, but the Trichogramma rice borer cannot complete the development after being parasitized

Method used

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  • Trichogramma japonicun ashmead artifical breeding production method

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Embodiment 1 produces beet armyworm egg

[0050] 1. For the rearing of beet armyworm, the temperature in the insectarium is controlled at 27±1°C and the humidity is about 65%.

[0051] The rearing of A larvae: Place the beet armyworm ovum that will hatch soon on one side of the petri dish, and add an appropriate amount of artificial feed on the other side, and cover with a lid with toilet paper to prevent the larvae from escaping. About 0.1 grams of eggs per dish. After 2 days of hatching, the larvae in one dish can be reared in 2 or 3 dishes separately; after another 2-3 days of growth, the larvae can be transferred to a large square box for feeding, and the larvae in 2-3 dishes can be combined into In a large square box, artificial feed is added; the number of larvae in each large square box can be adjusted according to the actual situation. Before pupation, there are about 400 larvae in each large square box, and the larvae of beet armyworm are about 12 days. Befo...

Embodiment 2

[0055] Embodiment 2 breeding Trichogramma rice borer

[0056] 1. The production of egg cards, the beet armyworm eggs used to receive bees are passed through a 40-mesh sieve, divided into single grains, and the eggs are poured on paper sheets coated with glue or glued with double-sided tape to make egg cards and dried in the air , (about 380 eggs of beet armyworm eggs per square centimeter), inactivated under 15 watts of ultraviolet light for 30 minutes.

[0057]2. The temperature of the breeding chamber is kept at 27±1°C, and the humidity is about 80%. Since the Trichogramma rice borer has phototaxis, the breeding chamber is generally dark when receiving bees. Put the prepared egg card into the bee-receiving box according to the bee-egg ratio of 1:10, and take it out after 1 day. Put in bee breeding and new egg cards every day. Place the egg cards that have been picked up at 27±1°C for 3 days until the Trichogramma rice borer develops to the pupae. Store in a refrigerator...

Embodiment 3

[0058] Embodiment 3 and the comparison of prior art and cost estimation

[0059] Trichogramma rice borer can parasitize the eggs of corn borer and armyworm, but the eggs of these two insects are in the form of lumps, which are not easy to remove from the egg-laying utensils to make egg cards, which limits the application; the life cycle of diamondback moth is short, and the reproduction Ligao can quickly expand the population in a short period of time and obtain a large number of eggs, but the eggs are small, resulting in a decline in the quality of the bred Trichogramma rice borer. Similarly, diamondback moth eggs are not easy to make egg cards and form standardization; Spodoptera litura The amount of eggs laid is large, and it is easy to make a standard egg card, but the Trichogramma rice borer cannot complete the development after being parasitized. Therefore, screening an alternative host with high production efficiency is an important basis for using Trichogramma as a b...

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Abstract

The invention provides a method for breeding trichogramma japonicun ashmead by means of beet armyworm ovums. The method is relatively high in production efficiency and short in breeding period, and is beneficial to scale production of trichogramma japonicun ashmead. The method comprises following steps of 1) producing beet armyworm ova; and 2) breeding trichogramma japonicun ashmead by means of the beet armyworm ova. The trichogramma japonicun ashmea bred artificially are used for controlling agricultural pests which mainly comprise rice-stem borer, yellow rice borer and rice leaf roller. Compared with the prior art, the trichogramma japonicun ashmead artifical breeding production method is advantaged in that beet armyworm can be obtained easily, and can be bred simply; and the method is relatively high in production efficiency and short in breeding period, and is convenient for large-scale breeding, and is beneficial to scale production of trichogramma japonicun ashmead.

Description

technical field [0001] The invention belongs to the technical field of biological control of agricultural pests, and relates to a breeding and production method for Trichogramma, in particular to an artificial breeding and production method for Trichogramma rice borer. Background technique [0002] Trichogramma is the most researched and most widely used insect in biological pest control in the world. At least 12 species have been artificially propagated in large numbers to control some important agricultural and forestry pests. The mass breeding of Trichogramma population is mainly carried out by using intermediate host eggs, and the intermediate host eggs of Trichogramma are relatively successfully utilized are tussah silkworm eggs, castor silkworm eggs, wheat moth eggs and rice moth eggs. Large eggs (tussah silkworm eggs, castor silkworm eggs) are easy to obtain and relatively low in cost, so large-egg bees bred with tussah silkworm eggs such as Trichogramma pine caterpil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 秦启联王红托舒锐豪李芳功方分分陈新中程清泉苗麟李瑄张寰张继红
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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