Tissue culture rapid propagation method of polygonatum multiflorum

A multi-flowered Polygonatum chinensis, tissue culture and rapid propagation technology, applied in the field of plant tissue culture, can solve the problems of accelerating the cultivation time proliferation coefficient and the like, and achieve the effects of shortening the tissue cultivation time, shortening the tissue culture time, and improving the proliferation coefficient.

Active Publication Date: 2018-04-03
ANHUI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Rhizome has very strong reproductive potential, can grow young shoots and root system, form new plant and carry out asexual reproduction, thereby prior art mostly uses the rhizome o

Method used

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  • Tissue culture rapid propagation method of polygonatum multiflorum
  • Tissue culture rapid propagation method of polygonatum multiflorum
  • Tissue culture rapid propagation method of polygonatum multiflorum

Examples

Experimental program
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Embodiment 1

[0058] Take the mature Polygonatum seeds collected from September to November, put them in a plastic bag and seal them for 2 weeks, the seed coat can be softened, rub the peel off, clean and put the seeds in a cool place to dry naturally. Mix the dried Polygonatum polyflora seeds with the sand and store them in sand at 5°C for 60 days. During the period of sand storage, spray water regularly to keep the humidity at 50-55%.

[0059] Soak Polygonatum polyflora seeds stored in sand in warm water at 35-40°C for 24 hours, and then soak them in gibberellin solution with a concentration of 100 mg / L for 22 hours, then take them out. Put Polygonatum polyflora seeds soaked in gibberellin solution in a beaker in an ultra-clean bench, disinfect with 75% alcohol by volume for 30 seconds, rinse with sterile water for 3 times, and rinse with 0.1% HgCl 2 Soak in the solution for 10min, then rinse 5 times with sterile water and inoculate the Polygonatum multiflora seeds in the seed cluster bud...

Embodiment 2

[0082] Take mature Rhizoma Polygonatum seeds collected from September to November, put them in plastic bags, seal and ferment for 3 weeks, the seed coats will soften, rub the peels off, clean them, and place the seeds in a cool place to dry naturally. Mix the dried Polygonatum multiflora seeds from which the pericarp has been removed with sand, store them in the sand at 4°C for 50 days, and spray water regularly to keep the humidity at 40-60% during the sand storage.

[0083] Soak Polygonatum polyflora seeds stored in sand in warm water at 30-40°C for 18 hours, then soak them in gibberellin solution with a concentration of 50 mg / L for 24 hours, and then take them out. Put Polygonatum polyflora seeds soaked in gibberellin solution in a beaker in an ultra-clean bench, disinfect with 75% alcohol by volume for 30 seconds, rinse with sterile water for 3 times, and rinse with 0.1% HgCl 2 Soak in the solution for 10 minutes, rinse with sterile water for 5 times, and then inoculate th...

Embodiment 3

[0089] Take mature Polygonatum seeds collected from September to November, put them in a plastic bag and seal them for fermentation for 2 weeks, the seed coat can be softened, rub the peel off, clean and put the seeds in a cool place to dry naturally. Mix the dried Polygonatum multiflora seeds with the peel removed with sand, store in sand at 8°C for 80 days, and regularly spray water during the sand storage to keep the humidity at 50-60%.

[0090] Soak Polygonatum polyflora seeds stored in sand for 32 hours in warm water at 30-35°C, then soak them in gibberellin solution with a concentration of 150 mg / L for 20 hours, and then take them out. Put Polygonatum polyflora seeds soaked in gibberellin solution in a beaker in an ultra-clean bench, disinfect with 75% alcohol by volume for 30 seconds, rinse with sterile water for 3 times, and rinse with 0.1% HgCl 2 Soak in the solution for 10 minutes, rinse with sterile water for 5 times, and then inoculate the Polygonatum multiflora se...

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Abstract

The invention provides and relates to the technical field of plant tissue culture, and provides a tissue culture rapid propagation method of polygonatum multiflorum. The method sequentially comprisesthe following steps of performing sand storage; performing soaking by warm water being 35 to 40 DEG C; performing gibberellin treatment; performing pretreatment on the polygonatum multiflorum seeds soas to break the seed dormancy; inoculating the pretreated seeds into a seed cluster bud inducing culture medium; directly inducing the seeds into cluster buds, wherein the seed cluster bud inducing culture medium is prepared from 1.5 to 2.5mg/L of MS culture media 6-BA, 0.5 to 1.5mg/L of GA3, 6.5 to 7g/L of agar and 30 to 35g/L of sucrose, and the pH value is 5.5 to 6.0; dividing the cluster budsand performing propagation culture so as to expand the cluster bud quantity; performing rooting culture and domestication transplanting to obtain polygonatum multiflorum stock seedlings. The method provided by the invention has the advantages that the dormancy of the polygonatum multiflorum seeds can be effectively broken; the seed germination stage is shortened to five months; the effects of short propagation period and germination rate improvement by 85 to 90 percent are achieved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture and rapid propagation of Polygonatum polyflora. Background technique [0002] Polygonatum cyrtonema (scientific name: Polygonatum cyrtonema Hua) is a perennial herb of the genus Polygonatum cyrtonema in the family Liliaceae. Oblong-lanceolate, umbel, perianth yellow-green, black berries, flowering from May to June, fruiting from August to October. The rhizome of Polygonatum multiflora can be used as medicine or as an ornamental plant. [0003] Polygonatum multiflora is a species of Polygonatum specified in the "Chinese Pharmacopoeia (2015)", which has the effects of invigorating qi and nourishing yin, strengthening the spleen, moistening the lungs, and nourishing the kidneys. In recent years, with the in-depth research on the efficacy of Polygonatum multiflora and the advent of health care products and medicines for Polygonatum multiflor...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 金青童金凤常艳蔡永萍李昌林孙蕊蕊李昌素王倩柴岳
Owner ANHUI AGRICULTURAL UNIVERSITY
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