42results about How to "Break hibernation" patented technology

The invention provides and relates to the technical field of plant tissue culture, and provides a tissue culture rapid propagation method of polygonatum multiflorum. The method sequentially comprisesthe following steps of performing sand storage; performing soaking by warm water being 35 to 40 DEG C; performing gibberellin treatment; performing pretreatment on the polygonatum multiflorum seeds soas to break the seed dormancy; inoculating the pretreated seeds into a seed cluster bud inducing culture medium; directly inducing the seeds into cluster buds, wherein the seed cluster bud inducing culture medium is prepared from 1.5 to 2.5mg/L of MS culture media 6-BA, 0.5 to 1.5mg/L of GA3, 6.5 to 7g/L of agar and 30 to 35g/L of sucrose, and the pH value is 5.5 to 6.0; dividing the cluster budsand performing propagation culture so as to expand the cluster bud quantity; performing rooting culture and domestication transplanting to obtain polygonatum multiflorum stock seedlings. The method provided by the invention has the advantages that the dormancy of the polygonatum multiflorum seeds can be effectively broken; the seed germination stage is shortened to five months; the effects of short propagation period and germination rate improvement by 85 to 90 percent are achieved.

The invention relates to the field of plant tissue culture, in particular to a method for quickly germinating phytolacca americana seeds and application of the method to aseptic seedling culture. The method comprises: soaking the phytolacca americana seeds into a concentrated sulfuric acid for a certain period, damaging the structure of phytolacca americana seed coats, breaking the sleep state of the seeds, and making the seeds be quickly subjected to imbibition and enter the germination state; simultaneously, utilizing the sterilization function of the concentrated sulfuric acid to perform sterilization treatment on the phytolacca americana seeds; subsequently, inoculating the aseptic phytolacca americana seeds on a culture medium selected by an experiment, adjusting the conditions such as temperature and illumination, and making the phytolacca americana seeds germinate quickly; and finally obtaining aseptic phytolacca americana seedlings. The method can quickly break through the sleep state of the phytolacca americana seeds, and greatly shorten the germination time and the seedling time of the phytolacca americana seeds. The invention provides a method for culturing the aseptic phytolacca americana seedlings for laboratory investigation.

The invention relates to a method and system for breeding plant seedlings under compound induction of a magnetic field and an electric field. The method comprises the following steps of: keeping a seedling bed at appropriate humiture, and applying an electric field 50-100KV and a magnetic field 500-4,000 gauss to seeds on the seedling bed for 10-60 minutes respectively; and when seedlings are 0-35 millimeters in height, applying an electric field 25-50KV and a magnetic field 500-4,000 gauss for 1-5 times respectively, every time for 5-30 minutes. Through experiments, magnetic and electric field induction parameters suitable for different plant seedlings are obtained. In the system, a heating tape is laid at the bottom of the seedling bed; a coil and a metal net or plate are arranged in a substrate on the seedling bed; seeds are spread on the substrate; another metal net or plate is arranged above a surface substrate; a metal meshed plate is connected with a direct current power supply generating electric field; the coil is connected with an alternating current power supply generating magnetic field; an intelligent control center is arranged; and a central processor is used for controlling the intensities of the magnetic and electric fields and the humiture of the seedling bed according to a command of a host. Due to the adoption of compound induction of the magnetic field and the electric field, the rate of emergence is high, the germinative force is good, the seedlings grow vigorously, the cultivating period is short, and the transplanting survival rate is high.

The invention discloses a plant seed yield increase processing device. The device comprises a processing tube and an ultrasonic functional unit arranged on the tube wall of the processing tube. A conveying screw is arranged in the processing tube, and one end of the conveying screw is connected with a driving motor. A feed inlet and a discharge opening are formed in the processing tube. The invention further discloses a plant seed yield increase processing system which at least comprises two plant seed yield increase processing devices. The discharge opening of the first plant seed yield increase processing device conveys processed seeds into the feed inlet of the second plant seed yield increase processing device. The device and the system have the advantages that the seeds can be sufficiently and evenly subjected to ultrasonic processing, the processing effect is effectively improved, and the yield can be increased by 10% after the seeds are processed by equipment for about 1 min; the conveying speed of screw conveying can be flexibly controlled, the blocking phenomenon is avoided, spiral blades on the screw can turn the seeds in the conveying process, and ultrasound irradiation is more uniform; a modular structure is basically obtained, installation is flexible, and large-scale production can be conveniently achieved.

The invention relates to a rescue method after ball spallation in big tree transplanting. The method adopts the treatment steps as follows: (1) pouring gaps of a soil ball by adopting mud mixed with a rooting agent after transplanting so as to form a whole; (2) swathing boughs of a tree trunk and below and trunk parts by adopting a plastic film until the bottom of the trunk; and (3) conducting transfusion treatment to the trunk. Leaves and/or branches with leaves of the tree can be further pruned towards special trees before transplanting, and anti-transpirant spraying and/or overshadowing tothe whole tree can be conducted after being transplanted. With the method, a root system can be better contacted with soil and water, new roots of the root system can be quickened, water and nutrientcan be timely supplemented, cells of the tree and enzyme activity can be stimulated, water transpirating of the tree can be slow down, the absorption strength of the tree body can be reduced, nutrition consumption can be decreased, the up and down balance of the tree can be increasingly achieved again, and the survival rate of the whole canopy transplanted tree can be improved.

The invention provides a planting method for watermelon in a greenhouse. The cultivation method comprises the following concrete steps: seed germination acceleration, seedling management, management in a rejuvenation period, and field management. The cultivation method for the watermelon provided by the invention has the advantages of rapid growth speed, high yield, good quality, bright red watermelon flesh, sweet and crisp pulp, etc.; and compared with a traditional cultivation method for the watermelon, the method provided by the invention has the advantages of simple process, convenient operation, good economic benefits and great popularizing value.

The invention discloses a method for increasing the germination rate of magnolia denudata seeds. The method includes the steps of firstly, conducting primary seed soaking, wherein the magnolia denudata seeds are poured into an NaOH solution to be soaked for 4-5 hours and then filtered out; secondly, conducting washing, wherein the seeds filtered out are put in a container and washed till the pH value of outflow clear water is neutral; thirdly, conducting secondary seed soaking, wherein washed seeds are put in a GA3 solution and a 6-BA solution to be soaked for 40-48 hours; fourthly, conducting stratification treatment, wherein seeds are covered with sand about 0.5-1 cm thick and put at a day and night temperature of 18-28 DEG C to be treated for 10-12 weeks so that dormancy of seeds can be eliminated. The magnolia denudata seeds can be treated in batches through the method, the germination rate can reach 85-95%, and the method has the advantages of being simple in procedure, few in chemical raw material type, low in chemical raw material consumption, short in seed treatment cycle and low in cost.

The invention relates to a flower cultivation field, and provides a method of promoting flowering of peony during the Spring Festival with low cost by using cold conditions. The method is characterized in that the peony seedlings are transplanted into flower pots in late September and early October; the whole roots of peony seedlings are sprayed with naphthoacetic acid solution before transplanting, and the root stems of peony seedlings are irrigated with gibberellin solution, then are dried for 3-5d; the transplanted peony seedlings are put under the outdoor shade condition for seedling recovery and adaptation, after the seedling recovery and adaptation, the seedlings are moved to the outdoor sun for adequate cultivation, when the outdoor temperature drops to minus 10-15 DEG C, the seedlings are moved into the greenhouse. After transplanting, the temperature of cold condition can break the dormancy temperature of peony to break peony dormancy under natural low temperature condition, and save the facilities and running cost of cold storage; after moving into the greenhouse, the peony dormancy has been broken, beginning to enter the flowering stage. The method regulates the greenhouse condition according to the different period of the peony flowering, and can blossom during the Spring Festival.

The invention discloses a method for planting bean sprouts in the technical field of vegetable planting. The method includes the steps that soybeans are selected, a nutrient solution is prepared; ferric citrate is prepared into a 500-1000 mg/L ferric citrate solution, and then sodium selenite is prepared into a 1.5-2.5 mg/L sodium selenite solution; the soybeans are soaked and boxed; spraying is conducted, wherein the soybeans are sprayed with a 300-400-fold diluent of em bacteria for 3-4 h; after the soybeans sprout, the ferric citrate solution and the sodium selenite solution are sprayed atthe same time for 3-4 h; the bean sprouts are picked, wherein the bean sprouts are taken out from a cultivation box and sprayed with clean water to be cleaned. The bean sprouts planted through the method are relatively high in iron content and selenium content.

The invention provides a tissue culture and rapid propagation method of populus lasiocarpa, and belongs to the technical field of plant tissue culture. The tissue culture and rapid propagation methodincludes subjecting branches of the populus lasiocarpa to refrigerating, soaking and water planting in sequence to obtain tender twigs 8-10cm in length; sterilizing the tender twigs to obtain sterileleaves; cutting the sterile leaves into small pieces, and inoculating the small pieces to an adventitious bud inducing medium for inducing culture to obtain adventitious buds; cutting the adventitiousbuds into stems with axillary buds, and inoculating each stem to a rooting medium for rooting culture to obtain rooted seedlings; transplanting the rooted seedlings after domestication to obtain populus lasiocarpa seedlings. The tissue culture and rapid propagation method of the populus lasiocarpa has the advantages that the steps are simple, the cycle is short, the annual propagation number of the leaves can reach 81, and tissue culture and rapid propagation of the populus lasiocarpa can be realized.

The invention discloses a method for increasing a survival rate of seedling culturing, planting and grafting of a honey pomelo, and relates to the technical field of fruit and vegetable planting. Themethod comprises the following steps of (1) activating seeds of the honey pomelo for further use: sowing the seeds of the honey pomelo into a seedling culture bed, fully watering the seeds, covering the seeds with a fresh-keeping film, transplanting young seedling grows with 10 to 14 true leaves into a field, and normally watering and applying fertilizer to the seedling, so as to obtain a parent tree; (2) manufacturing a cion, and putting the cion into a basin loaded with a nutritional liquid for further use; (3) selecting acid pomelo as a stock, shearing an upper part, cutting a skin layer from a smooth surface, 10 to 20 cm away from ground, of a main branch until the cion can be accommodated, inserting the cut cion into a cut of the stock, tightly attaching base parts of the stock and the cion, aligning the cutting surfaces, bundling the stock and the cion with a plastic film belt, timely removing sprout tillers after the cion survives, watering the cion at an interval of 2 to 3 days, and applying a compound fertilizer at an interval of 20 to 30 days. The seedling culturing, planting and grafting method has the advantages that the operation is simple, the implemention is easy, the survival rate of grafting is high, the growth is vigorous, and the output and quality of the honey pomelo are greatly improved.

The invention relates to the field of cucumber plantation technology, specifically to a seed treatment method for enhancing disease resistance of cucumber. The method comprises the following steps: S1, selection of seeds; S2, alkali treatment; S3, nutrient solution treatment; S4, electric field treatment; S5, pregermination. By the use of the above method, disease resistance and stress resistanceof seeds are effectively enhanced, seed growth is effectively promoted, absorption power of seeds is enhanced, nutrient loss or waste is further effectively avoided, and undesirable phenomena such assoil eutrophication or hardening, etc. due to nutrient residues are prevented.

The invention provides a germination accelerating method of sword bean seeds. The germination accelerating method includes: sterilizing, soaking the seeds, performing ultrasonic treatment, accelerating germination in a constant-temperature manner and sowing. The germination accelerating method has the advantages that the nutrition absorption of the seeds can be promoted, germination time is shortened, germ differentiation is benefited, the germination rate and stress resistance of the seeds are increased, planted sword beans are fresh and tender in quality, good in taste and high in nutritional value, and the germination accelerating method is simple in operation, capable of lowering planting cost and increasing economic benefits and suitable for large-scale production and popularization.

The invention discloses a culture method of wheat seedlings. The culture method comprises: wheat germination accelerating; with a porous sponge as a substrate carrier, injecting a culture medium into the porous sponge to obtain a sponge based culture medium, wherein the structure of the porous sponge is as follows: a blocky sponge is cut out to form a plurality of seed passages; each seed passage is in a curved shape, and the outlet of the seed passage is located in the upper surface of the blocky sponge, while the bottom of the seed passage is located in the blocky sponge; and carrying out seedling culture until the wheat seedlings are grown. According to the invention, using the porous sponge as the culture medium carrier, the problem of particle scattering everywhere of a sand-based culture medium is avoided; sufficient disinfection using hydrogen peroxide is ensured, thereby effectively preventing wheat seed mildewing, and water is not prone to loss and evaporation. The germination liquid and the culture medium used in the seedling culture process can quickly break the dormancy state of seeds and improves the wheat germination efficiency.

The invention relates to a germination method of camellia tree seeds. The method comprises (1) drying seeds in the sun for one day at 8th-10th day before sowing, soaking the seeds in clean cold waterfor 24h while kneading the seeds, then washing the seeds through clean water and carrying out rinsing to remove impurities until the water is clarified, (2) wrapping the seeds with gauze, squeezing toremove a part of water, putting the seeds into a refrigerator, keeping the temperature of 5 DEG C and washing the seeds twice in the morning and in the evening through clean water, (3) after 2 days,spread-drying the seeds in the light environment and washing the seeds twice in the morning and in the evening every day through cold water, wherein after each washing process, excessive water on theseeds is removed through blowing, (4) wrapping the seeds with gauze, hanging the seeds at a position above the water surface in a deep well, taking out the seeds every day, washing the seeds twice through clean water and drying the washed seeds through an electric fan and (5) carrying out treatment for 6 to 8 days to obtain the germinant seeds for sowing. The method shortens the germination time of the camellia tree seeds and the treated seeds have neat buds and a high germination rate.

The invention provides a germination accelerating method of Pinus thunbergii seeds. The method comprises the following steps: immersing the seeds in an immersion liquid, stirring the immersed seeds and peat, and carrying out poikilothermic treatment for 2-3d until the seeds germinate. The method breaks the state of dormancy, and is in favor of differentiating embryo seeds. Compared with routine methods, the method provided by the invention has the advantages of simple operation, shortening of the germination accelerating treatment time of the Pinus thunbergii seeds, increase of the germination rate, realization of regular germination, and allows the germination rate to reach 90% or above; and the germination accelerating method of Pinus thunbergii seeds also has the characteristics of great increase of the germination rate of the Pinus thunbergii seeds, plantation cost reduction, economic benefit increase, and suitableness for large-scale production and promotion.

The invention discloses a germination accelerating method for camellia oleifera seeds, and relates to the field of camellia oleifera planting. The germination accelerating method for the camellia oleifera seeds comprises the following steps: (1) selecting the camellia oleifera seeds with excellent quality for cultivation; (2) selecting full tea seeds, soaking in warm water at 40-45 DEG C, and draining; (3) soaking the tea seeds with a seed soaking agent for 10-15 hours, washing the tea seeds with clear water, and naturally air-drying the tea seeds; (4) soaking the tea seeds in a germination accelerating agent for 12 hours, draining the tea seeds and taking out the tea seeds; (5) refrigerating the tea seeds in a refrigerator at the temperature of -6-5 DEG C, taking out the tea seeds, and soaking the tea seeds in warm water; (6) wrapping the tea seeds by wet gauze, and putting the wrapped tea seeds into a thermotank at 25-30 DEG C for accelerating germination; (7) mixing tea seeds with wet sandy soil, putting the mixture into an air-raid shelter, and sowing after budding. The germination accelerating method for the camellia oleifera seeds is short in germination accelerating time, high in germination accelerating efficiency and capable of improving the disease and pest resistance of camellia oleifera in the subsequent growth process.