Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds

A kind of aseptic production and miniature technology, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve problems such as the impossibility of large-scale production, difficulty in seedling cultivation, and difficulty in cultivation and survival, so as to alleviate white and The effect of shortage of provenance, promotion of white and large-scale artificial planting, and overcoming the low survival rate of transplanting

Inactive Publication Date: 2013-02-06
建始县颐泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seeds are small, and they need to be symbiotic with fungi to germinate under natural conditions. The cultivation of seedlings is relatively difficult, and the survival rate of traditional vegetative reproduction methods such as branching and cutting is extremely low, and reproduction is very difficult. It is impossible to meet large-scale production needs
Although there are many people involved in the research on tissue culture and seed propagation technology of Bletilla japonicus, it basically takes the technical route of cultivating seedlings and then transplanting them to the field. After the tissue cultured seedlings of Bletilla japonicus leave the cultivation environment, it is difficult to cultivate and survive. its application in production

Method used

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  • Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds
  • Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds
  • Method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds

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Embodiment 1

[0025] The present invention is a method for aseptically producing miniature stems from Radix Radix Radix, a method for aseptically producing miniature stems from Radix Radix Radix, the main steps of which are as follows: (1) Germination of seeds and cultivation of sterile seedlings: harvesting mature Radix Radix Radix For fruits, first rinse with washing powder for 20 minutes, then put them in 2% mercuric solution for disinfection for 20 minutes, then rinse them with sterile water for 5 times, and then inoculate them. The inoculation method is as follows: cut the fruit in a cross shape, pop the white powdery seeds on the high-temperature sterilized filter paper, and then inoculate an appropriate amount on the medium. After inoculation, place at 24±2°C, light for 10 hours a day, and cultivate under light intensity of 20,000-30,000 lx. After 30 days to 100 days, the seeds germinate and grow into seedlings. The formula composition of the medium is KC+100-200g / L potato, and the p...

example ２

[0027] Example 2: According to the steps described in Example 1, wherein: step (1) the medium in seed germination and sterile seedling cultivation is 1 / 2 MS+10% coconut milk. Others are the same.

Embodiment 3

[0029] According to the steps described in Example 1, wherein: step (1) the medium in seed germination and sterile seedling cultivation is MS+2-4mg6-BA / L+0.2-0.4mgNAA / L. Others are the same. Example 4: According to the steps described in Example 1, wherein: step (3) the medium used for culturing the miniature stems is MS medium. Others are the same.

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Abstract

The invention relates to a method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds. The method comprises the following steps of disinfecting the common bletilla pseudobulb seeds; germinating the seeds on a germination culture medium; inducing the seeds to root; and inducing artificial seed stems to form, grow and mature and the like. According to the method, technical routes and key technologies from the step of germinating the common bletilla pseudobulb seeds to the step of forming the mature seed stems are optimized and proposed, and a technology of culturing common bletilla pseudobulb tissues can be simplified, so that the common bletilla pseudobulb seeds are directly are bred in a large scale, and the common bletilla pseudobulb seed stems are produced. The germination rate of embryo seeds reaches more than 95 percent, and more than 80 percent of tissue culture seedlings can form mature miniature seed stems. According to the method, the difficulty that the common bletilla pseudobulb seeds rarely germinate under natural conditions is overcome, and the defects that the survival rate of transplantation of the seedlings in the conventional common bletilla pseudobulb tissue culture and seed aseptical breeding is low and the like are overcome. A feasible practical technology is provided for quickly producing a great amount of seed stems in factories by means of the common bletilla pseudobulb seeds, the current situation of the shortage of the seed sources of common bletilla pseudobulb can be alleviated, and large-scale artificial cultivation of the common bletilla pseudobulb is promoted.

Description

technical field [0001] The invention relates to a method for aseptically producing miniature seed stems from bletilla seeds. Background technique [0002] White and Bletilla striata (Thunb. ex A. Murray) Rchb. f. of dry tubers. Also known as Baiji, Lianjicao, Ruolan, Gangen, Baigen, Baili, Zihui. Baiji has a long history of medicinal use and is a traditional Chinese medicine in my country. "Shen Nong's Materia Medica" contains: "Indications for the treatment of painful, swollen, malignant sores and gangrene, and injury to the dead muscles of the yin." ), scrofula, flutter damage, knife and arrow injury, promoting muscle growth and relieving pain. "Medicine Method Image" said: "Stop lung blood." Experimental studies have proved that Baiji decoction and powder have hemostatic effect, and have a certain effect on experimental gastric and duodenal perforation. Moreover, anal fissure and other diseases have good curative effects, and it is one of the main medicines for the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 谢玲玲
Owner 建始县颐泰生物科技有限公司
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