Clone seedling breeding method for sea-tangle gametocyte

A gametophyte and kelp technology, applied in botany equipment and methods, seaweed cultivation, plant cells, etc., can solve problems such as long seedling time, different development and decay of kelp species, mixed kelp varieties, etc., and achieve the goal of increasing production Effect

Inactive Publication Date: 2006-07-26
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Problems in this method: one is that the seedling time is affected by the water temperature in the sea area, and the seedling time is long; the other is that the kelp species are mixed when the kelp is raised; the third is that the development degree and decay of the kelp are different, resulting in the occurrence of different diseases in the seedling process.
[0003] Laminaria gametophyte cloning seedling raising method solves the above-mentioned problems, seedling raising technology has basically matured, but this new technology is difficult to popularize
The reason that causes this situation: the one, because this method requires higher kelp seedling raising technology, the 2nd, can only use single layer curtain in this method seedling raising process, and conventional method can have the double layer curtain of 30~40%, cause cloning Seedling production is reduced by more than 30% compared with conventional methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0014] Embodiment, a kind of kelp gametophyte clone breeding method, take Jiaodong Bohai Bay area as an example, on August 9, start circulating water to cool down, and the kelp seedling curtains that have been cleaned and pretreated are neatly placed in the nursery pond. On the second day, 30kg of pre-stimulated male and female gametophyte clones were smashed with a high-speed tissue masher, and then manually rubbed into small cell segments with a 400-mesh sieve, and sprayed evenly on 4,000 kelp seedlings with a sprayer. Fill the nursery pond with water before spraying the clones and stop the circulation. The clones are basically attached in the morning of August 12, and the water starts to circulate to provide suitable light, water temperature, water quality and nutrition to promote the development of clones; the gametophyte begins to develop on August 15. Sporophyte, on August 25, the sporophyte developed to 4 rows of cells, and some sporophytes were firmly attached. Use a wa...

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Abstract

This invention discloses a clonic seeding method of sea-tangle gametocyte. Comparing with former mature sea-tangle seed source, using gametocyte clone as seed source can solve these problems: seeding time is limited by sea areas and water temperature; seeding time is long; sea-tangle seeding causes its varieties complex; the growth degree and dote condition of sea-tangle are different, which causes various diseas occur during seeding period. The seeding-pick are proceed at twice and two curtains are employed, these heightens sea-tangle seeding output.

Description

1. Technical field [0001] The invention belongs to the technical field of marine biological seedling raising, in particular to a method for cloning seedlings of kelp gametophytes. 2. Background technology [0002] The kelp seedlings still use the conventional kelp breeding method. The method is to bring the pre-selected mature kelp into the room before the water temperature in the natural sea area rises to 23 ℃ (in mid-July in Zhifu Bay). Seawater stimulates the release of zoospores, and after reaching a certain density, the attached substrate is added. After swimming for a certain period of time, the zoospores actively attach to the substrate. Under the conditions of appropriate water temperature and light, they can start to grow and develop. After three months The kelp seedlings grow to 2 cm, and the natural water temperature in the sea area is stable below 19 ℃ (Zhifu Bay in mid-October). The kelp seedlings are moved out of the pool to the sea for temporary cultivation. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01G33/00
Inventor 车轼丛义周于松敏曲善村孙娟金瑜王青岩
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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