Quick propagation method of nervilia fordii seed balls

A technology of blue sky sunflower ball and blue sky sunflower, applied in the field of rapid propagation of blue sky sunflower seed balls, can solve the problems of high cost, long induction period, unfavorable factory production and the like

Inactive Publication Date: 2018-12-28
遵义市龙驰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, in the process of propagating the blue skyflower, it is induced in the bottle during tissue culture, but the induction medium is complicated, the induction period is long, and the c...

Method used

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  • Quick propagation method of nervilia fordii seed balls
  • Quick propagation method of nervilia fordii seed balls

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The rapid propagation method of blue skyflower seed bulbs comprises the following steps:

[0019] (1) Use the bulb of the blue skyflower as the explant, and germinate and cultivate the explant to obtain the stem. The specific operation is: use the bulb of the blue skyflower as the explant, inoculate it on the germination medium, place it at 26-28 ° C, Light intensity 2000Lx, 12h / d, cultivate for 30-35 days, and obtain walking stems;

[0020] (2) Subculture the stems on the proliferation medium, cut them into sections, lay them in the corm induction matrix, manage for 2 months, obtain the bulbs, and transplant them to the field. The specific operation is: cut the stems into 2- The 3cm-long section is inoculated on the proliferation medium, placed at 26-28°C, with a light intensity of 2000Lx, 12h / d, and cultivated for 30-40 days to obtain thick stems; wash the thick stems with tap water, cut into For the section of 3-5cm, use 1 tablet of aspirin and 1L water to prepare s...

Embodiment 2

[0022] On the basis of embodiment 1, adopt tap water to clean the thick and strong stems, cut into 3-5cm sections, and set aside; in the simple and easy shed preparation, form a wide 1m, long 5m seedbed, on the seedbed, lay a Layer 3-5cm thick corm inducing matrix, and spare thick and strong stems are laid on the corm inducing matrix, and others are all the same as embodiment 1.

Embodiment 3

[0024] On the basis of Example 1, the corm-inducing substrate is formed by mixing humus, perlite, decomposed sawdust, and grass charcoal at a volume ratio of 5.5:5.5:5.5:1.5. Others are all the same as in Example 1.

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Abstract

The invention relates to the technical field of culture of traditional Chinese medicine material seedlings, in particular to a quick propagation method of nervilia fordii seed balls. The quick propagation method comprises the following steps of performing explant germination and culturing, performing propagation culture medium subculturing, performing management culturing in corm induction mediums, and performing transplanting, so that nervilia fordii explants are induced outside a bottle to form corms. The propagation coefficient of nervilia fordii corms is increased, the production cycle isshortened, the inductivity of the corms is high, the operation is simple, and large-scale industrialized production is facilitated.

Description

technical field [0001] The invention relates to the technical field of raising seedlings of Chinese medicinal materials, in particular to a method for rapid propagation of blue skyflower seed bulbs. Background technique [0002] Blue Skyflower is a terrestrial orchid plant. The underground part of the plant has a nearly spherical to oval tuber, which is the main part for medicinal purposes. Tubers milky white, slightly transparent. The surface of tubers, rhizomes and roots is densely covered with cobweb-like symbiotic fungal silks. It is a rare Chinese herbal medicine with a long history of folk application. However, due to the small number of wild blue skyflowers, scattered distribution, and difficulty in finding, and the asexual reproduction of bulbs, the reproduction coefficient is extremely low, coupled with years of extinct excavations, leading to the depletion of wild blue skyflower resources. However, the demand in domestic and foreign markets is increasing year by...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 刘红美梁大勇石建龙
Owner 遵义市龙驰生物科技有限公司
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