The application provides a preparation method of
Artemisia sphaerocephala
pollen allergen and an
Artemisia sphaerocephala
pollen allergen injection, and belongs to the technical field of
pollen allergen vaccine preparation. After the
Artemisia sphaerocephala pollen is defatted and dried, the
Artemisia sphaerocephala pollen is extracted by using an extraction solution, the supernatant is filled into a
dialysis bag to be dialyzed, concentrated, enzymolyzed and desalted, and then
chromatography is carried out, and polypeptides with molecular weights of 23-46KD and 67-82KD are collected to be the
Artemisia sphaerocephala pollen allergen. The
Artemisia sphaerocephala pollen allergen injection comprises the Artemisia sphaerocephala
pollen allergen,
sodium chloride, PBS buffer,
phenol and
thiourea. The Artemisia sphaerocephala pollen allergen injection of the application fills the blank of Artemisia sphaerocephala pollen allergen injection, can effectively reduce the allergic degree of guinea pigs to Artemisia sphaerocephala pollen, and can be stably stored at 2-8 DEG C.