Novel method for quickly screening high-yield kelp strains

A new method and technology of kelp, applied in the field of kelp seed breeding, can solve the problems of a lot of manpower, material resources, blindness and randomness of breeding results, long experimental period, etc. Effect

Inactive Publication Date: 2014-09-17
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Therefore, the existing breeding methods not only have a long experimental cycle, which needs to go through 10 months, but also require a lot of manpower and material resources in the experimental process.
In addition, due to the influence of the sea area environment, there is a certain blindness and randomness in the breeding results, and not all the breeding results can achieve the ideal traits.

Method used

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  • Novel method for quickly screening high-yield kelp strains
  • Novel method for quickly screening high-yield kelp strains
  • Novel method for quickly screening high-yield kelp strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A new method for rapid screening of high-yield Laminaria strains, which uses chlorophyll fluorescence technology to detect and compare the photosynthetic system parameters of different strains of Laminaria gametophyte photosynthetic system II,

[0027] 1) The different strains of kelp used in the experiment were collected from the kelp breeding area of ​​Sanggou Bay, Rongcheng City, Shandong Province, and were labeled ZS, BN, JA and JB respectively.

[0028] 2) The composition and final concentration of the PES medium used in the experiment process are: sodium nitrate (NaNO 3 ) 46mg / L, glycerophosphate (C 3 h 7 Na 2 o 6 P·5H 2 O) 6.66mg / L, boric acid (H 3 BO 3 )3.8mg / L, 0.5M ethylenediaminetetraacetic acid (C 10 h 16 N 2 o 8 ) 18.2ml / L, Trismabase (C 4 h 11 NO 3 )66.6mg / L, ferric ammonium sulfate hexahydrate ((NH 4 ) 2 Fe(SO 4 ) 2 ·6H 2 O) 2.34mg / L, ferric chloride (FeCl 3 ) 100μg / L, manganese sulfate monohydrate (MnSO 4 ·H 2 O) 546μg / L, zinc sulfat...

Embodiment 2

[0034] Embodiment 2 zoospore breeding and sporophyte sea area cultivation:

[0035] The kelp sporophyte seedlings of different strains of Example 1 are cultivated by traditional zoospore breeding technology, and after reaching the specification of the seedlings out of the warehouse (about 2 cm in length), they are transferred to the kelp breeding area of ​​Sanggou Bay, Rongcheng, Shandong Province in October, and are temporarily raised until one month later. , the seedlings were cultivated until harvesting in May of the following year, and the wet weight of kelp was detected monthly from January to May 2013. Maximum photosynthetic quantum yield F of Laminaria gametophytes of different strains v / F m and the actual quantum yield Y(II) as figure 1 shown. Such as figure 2 As shown, the wet weight of different strains of kelp increased significantly with the culture time (p image 3 As shown, as of May, the yields per mu of 4 different strains of ZS, BN, JA and JB were 23.168±...

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Abstract

The invention discloses a novel method for quickly screening high-yield kelp strains, and belongs to the technical field of kelp seedling breeding. Photosynthetic system parameters of photosynthetic systems II of gametophytes of different strains of kelps are detected and compared by a chlorophyll fluorescence technology, and the strains with large parameter values are preferably selected to be retained. The chlorophyll fluorescence technology adopted by the novel method disclosed by the invention is a high-efficiency and lossless detection technology; according to the fluorescence detection, the number of required gametophyte samples is small (fresh weight: 0.05g), and the gametophytes cannot be badly affected; the detected samples can be continued to be used for gametophyte cloning breeding; the new strains of the high-yield kelps are screened by detecting the photosynthetic parameters of the gametophytes; compared with the conventional seedling breeding method, the novel method has the advantages that the breeding time is greatly saved, and a breeding result is high in pertinence.

Description

Technical field: [0001] The invention belongs to the technical field of kelp seed selection and breeding, and in particular relates to a new method for quickly screening high-yield kelp strains. Background technique: [0002] Kelp (Saccharina japonica) belongs to Phaeophyta, Phaeosporae, Laminariales, Laminariaceae, and Saccharina in classification. It is a cold-water macroeconomic algae, mainly distributed in the Pacific and northern Atlantic coasts, and is an important part of the coastal marine ecosystem. Kelp is not only a nutrient-rich marine vegetable, but also rich in economic components such as alginate, mannitol, iodine, and salina. It is an important raw material for seaweed chemical industry, medicine and health care, and agricultural fertilizer industries. [0003] There was no kelp distribution in my country, and it was not until 1927 that kelp was successfully introduced into my country. Since the 1950s, my country has carried out research on the genetic impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
Inventor 徐东叶乃好张晓雯范晓
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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