New method for culturing kelp seedlings in scale without substratum

A new method, non-attaching technology, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problems of a large amount of manpower, low survival rate, seedling shedding, etc., to reduce losses, simple operation, and increase survival rate Effect

Inactive Publication Date: 2013-11-20
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0004] The traditional way of growing macroalgae is to cultivate seedlings by attaching zoospores to the substrate. However, this method of raising seedlings is limited by mature vegetables, and the seedling time is limited. Seedling cultivation requires a specific seedling substrate and a seedling workshop, requiring a lot of manpower, material resources
In addition, due to the secondary attachment process of macroalgae after rhizoid shedding, traditional seedling raising methods generally have the phenomenon of serious seedling shedding and low survival rate

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  • New method for culturing kelp seedlings in scale without substratum

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Embodiment

[0025] The experiment started on July 18, 2012 and ended on December 20, 2012. The results of culturing macroalgae young sporophytes by aeration and suspension method showed that the macroalgae embryosporophytes obtained by gametophyte cloning and breeding technology were then tested in the laboratory. Cultivate macroalgae young sporophyte seedlings with a growth of about 1-2cm under controlled conditions.

[0026] (1) Acquisition of macroalgae embryosporophyte: The method of gametophyte cloning hybrid breeding (new method for breeding macroalgae seedlings by gametophyte cloning method 201110373578.8) was used to obtain embryosporophyte. The male and female gametophytes of macroalgae were obtained from the germplasm resource bank of the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences. From July 18, 2012 to September 20, 2012, a large number of male and female gametophytes of macroalgae ( figure 1 A).

[0027] (2) Development of macroalgae e...

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Abstract

A new method for culturing kelp seedlings in scale without substratum includes steps of (1) obtaining kelp embryoid sporophore by means of gametophyte clone cross breeding, (2) moving mixed liquid containing the kelp embryoid sporophore into sterile PES culture media to prepare embryoid sporophore culture solution and breeding the kelp embryoid sporophore into kelp young sporophore of 200+/-20 micrometers, and (3) expanding a kelp young sporophore culture system gradually by a three-step culture method, increasing culture temperature, breeding the young sporophore into seedlings of 1-2cm gradually in culture containers by a ventilation suspension method. By the ventilation suspension method, the survival rate can be increased greatly and loss caused by rotten seedlings can be reduced. Due to the ventilation suspension method for culture of kelp, operation in kelp culture is simple, laborsaving and timesaving, limitation in mature main weed is avoided, the kelp seedlings can be cultured anytime according to actual requirements, and the survival rate and the culture density of unit water body are higher than those of conventional methods.

Description

technical field [0001] The invention belongs to the technical field of algae breeding, and in particular relates to a new method for large-scale cultivation of giant algae seedlings without attachment base. Background technique [0002] Giant algae [Macrocystis pyrifera (L.) Ag.] belongs to Phaeophyta, Laminariales, Lessoniaceae, and Macrocystis in classification, and is the largest individual in the world. Seaweed is one of the fastest growing plants in the world. Macroalgae occupies a very important position in the natural ecosystem, and is the habitat of various marine organisms such as abalone, fish and other sea treasures, and plays a positive role in maintaining the ecological balance near the shore and preventing the occurrence of large-scale animal diseases; At the same time, giant algae has important application value and is used to produce a variety of chemical and pharmaceutical products, and is an important raw material for the production of alginate and methane...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 叶乃好徐东王依涛张晓雯
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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