New method for culturing kelp seedlings in scale without substratum

Abstract

A new method for culturing kelp seedlings in scale without substratum includes steps of (1) obtaining kelp embryoid sporophore by means of gametophyte clone cross breeding, (2) moving mixed liquid containing the kelp embryoid sporophore into sterile PES culture media to prepare embryoid sporophore culture solution and breeding the kelp embryoid sporophore into kelp young sporophore of 200+ / -20 micrometers, and (3) expanding a kelp young sporophore culture system gradually by a three-step culture method, increasing culture temperature, breeding the young sporophore into seedlings of 1-2cm gradually in culture containers by a ventilation suspension method. By the ventilation suspension method, the survival rate can be increased greatly and loss caused by rotten seedlings can be reduced. Due to the ventilation suspension method for culture of kelp, operation in kelp culture is simple, laborsaving and timesaving, limitation in mature main weed is avoided, the kelp seedlings can be cultured anytime according to actual requirements, and the survival rate and the culture density of unit water body are higher than those of conventional methods.

Description

technical field [0001] The invention belongs to the technical field of algae breeding, and in particular relates to a new method for large-scale cultivation of giant algae seedlings without attachment base. Background technique [0002] Giant algae [Macrocystis pyrifera (L.) Ag.] belongs to Phaeophyta, Laminariales, Lessoniaceae, and Macrocystis in classification, and is the largest individual in the world. Seaweed is one of the fastest growing plants in the world. Macroalgae occupies a very important position in the natural ecosystem, and is the habitat of various marine organisms such as abalone, fish and other sea treasures, and plays a positive role in maintaining the ecological balance near the shore and preventing the occurrence of large-scale animal diseases; At the same time, giant algae has important application value and is used to produce a variety of chemical and pharmaceutical products, and is an important raw material for the production of alginate and methane...

AI Technical Summary

Problems solved by technology

[0004] The traditional way of growing macroalgae is to cultivate seedlings by attaching zoospores to the substrate. However, this method of raising seedlings is limited by mature vegetables, and the seedling time is limited. Seedling cultivation requires a specific seedling substrate and a seedling workshop, requiring a lot of manpower, material resources

In addition, due to the secondary attachment process of macroalgae after rhizoid shedding, traditional seedling raising methods generally have the phenomenon of serious seedling shedding and low survival rate

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