Method for simultaneously extracting high quality DNA/RNA of kelp gametophyte
A gametophyte, high-quality technology, applied in the field of molecular biology, can solve the problems of liquid nitrogen grinding, small DNA fragments, large amount of materials, etc., and achieve the effects of convenient material acquisition, avoiding interference and saving costs.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] A method for simultaneously extracting high-quality Laminaria gametophyte DNA / RNA, which includes material selection, multiple grinding and cracking of materials, extraction, precipitation and dissolution, and purification of DNA / RNA; the specific operations are as follows:
[0030] Take fresh kelp gametophytes and wash them in filtered seawater, measure the fluorescence parameter Fv / Fm value of gametophyte chlorophyll, and select the gametophytes with Fv / Fm value between 0.3-0.6.
[0031]1g gametocytes are placed in mortar, add and contain polyvinylpyrrolidone (PVP) and dithiothreitol (DTT) CTAB lysis buffer 10ml, the final concentration of PVP and DTT in CTAB lysis buffer is respectively 2% (mass ratio) and 50mM. Grind until the tissue is dissolved; divide the tissue lysate into six 2ml Ep tubes, add two 3mm steel balls to the Ep tubes, vortex, and grind for 20min with a tissue grinder at 10°C; then centrifuge (45sec , 8100×g), collected the supernatant in a 50ml cen...
Embodiment 2
[0041] In this embodiment, the conventional liquid nitrogen grinding method is used to extract the DNA in the gametophyte, and the remaining steps are completely the same as in Example 2, and finally the DNA solution is obtained, and the quality of the nucleic acid sample is detected by electrophoresis on a 1% agarose gel, as shown in FIG. Figure 5 .
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com