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In-vitro rapid propagation method of macromitrium cavaleriei card and ther

A technology of moss chinensis and gametophyte, which is applied in the field of bioengineering to achieve the effects of highly intensive and high-density factory production, automatic control production, and obvious scientific research and application value

Inactive Publication Date: 2014-08-27
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the prior art, there is no culture technique for a large number of gametocytes in test tubes and flasks, and there is no relevant research report on obtaining gametophytes by culturing S. sinensis isolates.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Cut the spore capsules of Moss sinensis growing in the natural environment for surface disinfection, and the disinfectant is 75% alcohol solution and 0.1% mercury solution by volume; after disinfection, inoculate the spores in the sterile spore capsules into The modified Knop's medium without any hormone was cultured to obtain sterile protoceles, and after another 30 days of culture, the protoceles were differentiated to obtain gametophytes. The culture conditions are: temperature 23° C., light intensity 2000 lx, light time 14 hours / day, and observe the culture results after 10 days of culture.

[0019] Prepare 75% alcohol solution by volume fraction and mercuric chloride solution with 0.1% mass volume fraction according to the conventional method, and use for the surface sterilization of the gametophyte of Moss sinensis, the sterilant combination and sterilization time are the following 3 kinds:

[0020] a) 75% volume fraction of alcohol solution for 30 seconds and 0.1...

Embodiment 2

[0031] The single gametocyte strain obtained in Example 1 was inoculated on the culture medium for gametocyte proliferation and multiplication, and cultivated for 30 days. The culture conditions are: temperature 23° C., light intensity 2000 lx, light time 14 hours / day.

[0032] Prepare the medium for inducing differentiation gametocytes to produce according to conventional methods, and the medium formula consists of the following 14 types:

[0033] a) Improved Knop's basic medium+10g / L sucrose+6g / L agar powder;

[0034] b) 1 / 2 improved Knop's basal medium (the amount of macroelement compound is 1 / 2 of the prescribed amount in the formula)+10g / L sucrose+6g / L agar powder;

[0035] c) 1 / 3 improved Knop's basal medium (the amount of macroelement compound is 1 / 3 of the prescribed amount in the formula)+10g / L sucrose+6g / L agar powder;

[0036] d) 1 / 4 improved Knop's basal medium (the amount of macroelement compound is 1 / 4 of the prescribed amount in the formula)+10g / L sucrose+6g / L...

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PUM

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Abstract

The invention discloses an in-vitro rapid propagation method of macromitrium cavaleriei card and ther, which comprises the following steps: (1) carrying out surface sterilization of mature capsula of the macromitrium cavaleriei card and ther which grows in the natural environment; inoculating the mature capsula on Knop's culture medium without any hormone to culture and obtain protonemata; and then culturing for 30 days to obtain gametophyte; (2) inoculating single gametophyte on a culture medium for subculture multiplication of the gametophyte to obtain new gametophyte. The invention builds the in-vitro rapid propagation method of macromitrium cavaleriei card and ther for the first time; by using the in-vitro rapid propagation method, large-scale artificial propagation of the gametophyte of the macromitrium cavaleriei card and ther can be realized in a short time. The method has obvious scientific significance and practical value, and can provide a large number of uniformly-growing ideal test materials for indicating and monitoring atmospheric pollution by utilizing bryophytes. Simultaneously, the method continuously provides rich abundant seedlings for garden landscapes which use the bryophytes, such as establishing of a moss garden.

Description

technical field [0001] The invention relates to a method for rapidly propagating gametophytes of Moss sinensis in vitro by using plant tissue culture technology, and belongs to the technical field of bioengineering. Background technique [0002] The genus Macramitrium Bridel in the family Orthotrichaceae is a group with pantropical distribution. There are very rich species of Moss in China, and there are currently more than 40 species accepted. Among them, Macromitrium cavaleriei Card. & Thér. is a new species described by Cardot in 1906 based on the specimens collected by J. Cavalerie from Guizhou (Kouy Techeou). It is recorded in a narrow distribution area in Southwest my country. The main characteristics of this species are The tree is epiphytic, the stems and leaves are often reflexed or close to the stems when they are wet, elliptic-lanceolate to triangular-lanceolate, apex acuminate; branches and leaves are linear or oval-lanceolate to lanceolate, most gradually acumin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCY02A40/22
Inventor 娄玉霞丁雪郭水良
Owner SHANGHAI NORMAL UNIVERSITY
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