Multi-control sterility expression vector constructed on basis of Ms7 gene and method for using multi-control sterility expression vector for keeping and reproducing corn recessive genic male sterile lines

Abstract

The invention discloses a multi-control sterility expression vector which is constructed through an Ms7 gene and used for recovering, keeping and reproducing corn recessive genic male sterile lines, and an application method of the multi-control sterility expression vector, and belongs to the field of plant genetic engineering in the biotechnology. The multi-control sterility expression vector mainly comprises four functional elements, namely, the fertility reproducing gene functional element for corn male sterility, the functional element used for restraining corn male gametophyte formation, the cone peel color screening and marking functional element, and the herbicide resistance gene functional element. The constructed vector is introduced into corn receptors such as cells, callus or organs, can be used for recovering, keeping and reproducing the male sterility lines of corn, is expected to be applied to corn sterile cross breeding and cross hybrid seed production, and generates great economical value in the corn production.

Description

technical field [0001] Generally, the present invention is in the fields of molecular biology and plant genetic engineering. Specifically, the present invention relates to a fertility restorer gene for restoring male sterile maize fertility Ms7 The construct, the recombinant vector comprising the construct, and the host cell transformed with the construct or the recombinant vector, the transgenic plant cell and the preparation method of transgenic corn, and the maize male sterile line seed and maintainer line produced therefrom seed. Background technique [0002] Heterosis is the phenomenon in which a hybrid is superior to its two parents in one or more traits. For example, the first generation of hybrids obtained by crossing different strains, different varieties, or even different species often exhibit stronger growth rate and metabolic functions than their parents, resulting in developed organs, increased body size, increased yield, or resistance to disease. Improvemen...

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Problems solved by technology

Artificial detasseling is relatively easy, and mechanical detasseling and chemical detasseling can also be used. However, these strategies also have some problems: on the one hand, these techniques greatly increase the cost of seed production; Thorough or untimely, it will reduce the purity of hybrids, resulting in a large area of ​​production reduction, and ultimately lead to great economic losses

[0004] Maize recessive male sterile materials do not have the problem of detasseling, but due to the lack of effective maintenance and propagation techniques, it is difficult to apply them in actual production

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