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Sterilized spore germination of platycerium wallichii and test tube seedling cultivating method

A technology of staghorn fern and spores, which is applied in the field of aseptic spore germination and test tube seedling propagation and cultivation of staghorn fern, can solve the problems of large-scale tissue culture production, etc., and achieve novel propagation methods, unique medium components, and high output Effect

Inactive Publication Date: 2008-03-05
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on its large-scale tissue culture production and patent application at home and abroad

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Collect the mature spores on the back of sporophyll of robust plants of Platycerium wallichii Hook cultivated in the greenhouse, soak them in 70% alcohol for 10 seconds, take them out and place them in 0.2% mercuric chloride solution for disinfection for 10 minutes, wash them with sterile water for 5 times, and inoculate To the spore germination medium (each liter contains 0.5g of activated carbon, 50mL of coconut milk, 0.1mg of gibberellin, 7g of agar, and the rest is MS), and cultured in the dark at 25°C for 2 weeks, and then cultured under 1500 1x light for 12 hours a day After 5 weeks, the prothallus formed. Transfer the prothallus to the gametophyte growth medium (each liter contains 0.1g of hydrolyzed casein, 0.2mg of 6-benzylpurine, 0.1mg of naphthaleneacetic acid, 7g of agar, and the rest is MS), at 25°C, 12 hours a day, 1500lx light Under culture for 5 weeks, a large number of clustered leaf-like gametophytes were formed. Inoculate the clustered thallus into l...

Embodiment 2

[0020]Collect mature spores on the back of sporophylls of robust plants of Platycerium grande cultivated in the greenhouse, soak them in 75% alcohol for 10 seconds, take them out and place them in 0.1% mercuric chloride solution for disinfection for 15 minutes, wash them with sterile water for 6 times, and inoculate the spores Germination medium (each liter contains 1g of activated carbon, 150mL of coconut milk, 0.5mg of gibberellin, 5g of agar, and the rest is MS), cultured in the dark at 24°C for 3 weeks, and then cultivated for 4 weeks under 2000lx light for 8 hours a day to form prothallus. Transfer the prothallus to the gametocyte growth medium (each liter contains 0.3g of hydrolyzed casein, 1.0mg of 6-benzylpurine, 0.5mg of naphthaleneacetic acid, 5g of agar, and the rest is MS), at 24°C, 8 hours a day, 2000 1x Cultivate under light for 4 weeks, and form a large number of clustered leaf-like gametophytes. Inoculate the clustered thallus into the liquid gametophyte growt...

Embodiment 3

[0022] Collect the mature spores on the back of the sporophyll of robust plants of Pla tycerium bifurca tum C.Chr. cultivated in the greenhouse, soak them in 70% alcohol for 10 seconds, take them out and put them in 1.5% sodium hypochlorite solution for disinfection for 10 minutes, and wash them with sterile water for 5 minutes. After the second time, inoculate into the spore germination medium (each liter contains 0.5g of activated carbon, 100mL of coconut milk, 0.2mg of gibberellin, 7g of agar, and the rest are MS), and cultivate in the dark at 26°C for 2 weeks, and then 1800lx for 10 hours every day Cultivate under light for 5 weeks to form prothallus. Transfer the prothallus to the gametocyte growth medium (each liter contains 0.2g of hydrolyzed casein, 0.5mg of 6-benzylpurine, 0.2mg of naphthaleneacetic acid, 7g of agar (with?), the rest is MS), 26°C, every day Cultivate under 1800lx light for 10 hours for 5 weeks to form a large number of clustered leaf-like gametophytes...

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PUM

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Abstract

The present invention relates to a method for propagation and cultivation of platycerium. It is characterized by that said invention adopts sterile spore germination technique and makes it be combined with test-tube seedling-cultivating technique to implement propagation and cultivation of platycerium. Said method includes the following several steps: collecting storing, health and mature spore, making sterile spore germination, gametophyte growth culture, insemination to form zygoite, sporophyte enrichment culture, rooting culture and transplantation, etc.

Description

technical field [0001] The invention relates to a method for propagating and cultivating staghorn fern, in particular to a method for propagating and cultivating staghorn fern by germination of sterile spores and test tube seedling cultivation. Background technique [0002] Staghorn fern, also known as staghorn fern, bat fern, staghorn fern, etc., is a plant of the genus Platycerium in the family Water Keelaceae. It is the most peculiar type of ornamental fern and belongs to the epiphytic ornamental fern. There are two types of leaves of staghorn fern. The vegetative leaves are small, round, oval or fan-shaped, and are closely attached to the attached organisms. Tender green, turning light brown when ripe, very cute. Staghorn fern is very popular in the decoration and arrangement of parks, botanical gardens, shops, living rooms, windowsills and other places in Europe and America. It is a rare and precious indoor foliage plant, especially a good material for indoor three-dim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 陈之林段俊吴坤林郑枫曾宋君
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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