Method for identifying resistance of tobacco to root rot caused by Fusarium tabacinum

Abstract

The invention discloses a method for identifying the resistance of tobacco to root rot caused by Fusarium tabacinum. The method comprises 1, culture of Fusarium tabacinum, 2, preparation of a Fusariumtabacinum liquid, 3, cultivation of tobacco seedlings, 4, pre-treatment on the tobacco seedlings, 5, inoculation with the Fusarium tabacinum, and 6, investigation to detect the resistance of the specie to the root rot caused by Fusarium tabacinum according to the disease conditions. The identification method can directly identify tobacco seedlings. Compared with the method for inoculation of a pot or a field with bacteria, the method is free of a complex process for pot culture after transplant, reduces work load, utilizes floating plate vaccination to inoculate with hundreds or thousands ofplants, enlarges the inoculation amount, greatly reduces identification time through direct inoculation in the seedling period, breaks the season limitation of the resistance of tobacco to root rot caused by Fusarium tabacinum and realizes identification in any seasons.

Description

technical field [0001] The invention relates to the technical field of tobacco disease identification, in particular to an identification method for tobacco's resistance to tobacco fusarium root rot. Background technique [0002] Tobacco is an important economic crop, and there are many kinds of diseases, among which the root diseases are the most serious. Black shank has been the most common tobacco root disease for a long time. In recent years, with the expansion of continuous cropping area, rhizome diseases have become more and more serious. In addition to black shank, a new disease is spreading, that is, Fusarium root rot. The occurrence of black shank has attracted high attention, so in-depth research has been done on variety breeding, resistance identification, cultivation measures, drug control and other means. Fusarium root rot was discovered on tobacco late, and its research mainly focused on the identification and isolation of pathogenic bacteria, and the researc...

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Problems solved by technology

[0006] Current identification method: At present, there is no identification standard for Fusarium root rot resistance, what can be found in the literature is the inoculation method about this disease in other research species, a kind of is to utilize Fusarium crude toxin to process after extraction and purification The newly sprouted tobacco seedlings (Study on the pathogenic crude toxin of Fusarium tobacco root rot pathogen, Zhao Jie); the other is to inoculate the tobacco seedlings at the 10-leaf stage with the bacteria cake, and prick the wound with an inoculation needle on the roots of the tobacco seedlings , paste the prepared fungus cake on the wound, dipped in absorbent cotton with sterile water and wrapped it on the inoculation site (preliminary molecular identification of Fusarium tobacco in Henan, Tian Yanyan), these two methods can achieve the purpose of making the tobacco plant disease, However, it does not play a role in identifying the degree of disease of the variety

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