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Identification method for clubroot resistance of non-heading cabbages

A technology of head cabbage and identification method, which is applied in the field of identification of non-heading cabbage resistance to clubroot, can solve the problems of serious root disease of non-heading cabbage, loss of vegetable growers, and decline in yield, etc., so as to improve the resistance to clubroot Virulence, strong anti-infection, strong sensitivity

Inactive Publication Date: 2015-03-11
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the vegetables are infected, the yield will decrease and the quality will decrease. In severe cases, soft rot bacteria and other pathogenic bacteria will take advantage of the situation and infect the affected parts, eventually causing the plants to wither and die, causing great losses to vegetable farmers.
[0005] At present, there are many researches on cruciferous clubroot at home and abroad, but they mainly focus on Chinese cabbage, cabbage, rape and radish. There are few studies on the identification methods of resistance to clubroot in non-heading cabbage infected with clubroot, and the root disease of non-heading cabbage is becoming more and more serious. Screening of anti-clubroot pathogenic materials of head cabbage provides technical support

Method used

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  • Identification method for clubroot resistance of non-heading cabbages
  • Identification method for clubroot resistance of non-heading cabbages
  • Identification method for clubroot resistance of non-heading cabbages

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1, Identification and analysis of non-heading Chinese cabbage infected with clubroot in different periods

[0027] The non-heading cabbage mainly grown in Shanghai, the conventional variety Xin Aiqing and the heat-resistant variety Xinxiaqing, were treated with root clubroot suspension at the same time and concentration at different growth stages (cotyledon stage and three-leaf one-heart stage). deal with.

[0028] Test method: The test was carried out in the greenhouse of the test base of the Institute of Horticulture, Shanghai Academy of Agricultural Sciences. First, the fresh diseased tissues of clubroot of non-heading cabbage (that is, the root tumor of non-heading cabbage) were collected at Hongyang Farm, Qingpu District, Shanghai, washed and packed in plastic bags, and stored in a -20°C refrigerator . During the test, the root tumor was taken out, homogenized with a plant tissue grinder, filtered with four layers of gauze, centrifuged at 4000r / min in a r...

Embodiment 2

[0035] Embodiment 2, identification of clubroot resistance of non-heading Chinese cabbage of different varieties

[0036] Test materials: All the materials in the examples are provided by the Qingcai Research Group of the Institute of Horticulture, Shanghai Academy of Agricultural Sciences. Among them, the numbers are 13-1g-1 to 13-1g-52 and 13-1g-54 to 13-1g-61, all of which are inbred line materials purified by the research group for many years; the numbers are 13-1g-65 to 13 -1g-70 is the intermediate material; numbers 13-3g-1 to 13-3g-3 are the clubroot resistant Chinese cabbage varieties introduced by South Korea's Sunnis Seed Company.

[0037]Test method: The test was carried out in the greenhouse of the test base of the Institute of Horticulture, Shanghai Academy of Agricultural Sciences. First, the fresh diseased tissues of clubroot of non-heading cabbage (that is, the root tumor of non-heading cabbage) were collected at Hongyang Farm, Qingpu District, Shanghai, washe...

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Abstract

The invention discloses an identification method for clubroot resistance of non-heading cabbages. The identification method comprises the following steps: preparing a clubroot bacterium suspension with the concentration of 1*10<7> to 2*10<8>bacteria / mL; when seedlings of the non-heading cabbage grow to have three leaves and one heart, uniformly irrigating the roots of the seedlings with the prepared clubroot bacterium suspension so as to soak the roots of the seedlings in the clubroot bacterium suspension for 3-4 days; taking the seedlings out of the clubroot bacterium suspension, culturing the seedlings for 40-50 days by a conventional method, and identifying and screening non-heading cabbages which are not infected by clubroot. By the identification method, when the seedlings of the non-heading cabbages grow to have three leaves and one heart, the seedlings are inoculated with clubroot bacteria by a root dipping method; by investigating the illness occurrence situation of plants, the disease-resistant or infected non-heading cabbages are identified.

Description

technical field [0001] The invention belongs to the technical field of disease resistance identification of cruciferous crops, and in particular relates to an identification method of non-heading cabbage resistance to clubroot. Background technique [0002] Clubroot of cruciferous crops is a root disease of cruciferous crops caused by the infestation of plasmodiophora brassicae Woron, an obligate parasite that specifically infects cruciferous crops. The pathogen invades the root hairs, cortex, and cambium of plants, produces pathogenic secretions containing plant growth hormone, stimulates the abnormal division and enlargement of parenchyma cells, and expands to form nodular roots at the hyperplasia. On the one hand, the appearance of swollen roots reduces the ability of the root system to absorb and supply water and nutrients, causing the aboveground part to lose water, wither and die; on the other hand, the plant root system loses the protective effect of the outer layer a...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/645
Inventor 朱红芳朱玉英李晓锋
Owner SHANGHAI ACAD OF AGRI SCI
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