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Method for evaluating resistance of brassica plants on club root

A technology for clubroot and clubroot, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problem of clubroot disease, which is difficult to ensure the consistency of identification conditions, difficult to complete large-scale variety identification, and time-consuming and other problems, to achieve the results of accurate and reliable identification results, strong practicability, and easy operation

Inactive Publication Date: 2012-06-27
云南省农业科学院园艺作物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects and deficiencies in the prior art that it is difficult to ensure the consistency of identification conditions, labor-intensive

Method used

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  • Method for evaluating resistance of brassica plants on club root
  • Method for evaluating resistance of brassica plants on club root
  • Method for evaluating resistance of brassica plants on club root

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] experiment material:

[0043] Varieties to be identified are: Chinese cabbage C09-35, Chinese cabbage C09-36, Chinese cabbage C09-37, Chinese cabbage C09-38,

[0044] The control is: Chinese cabbage 83-1, a high-susceptibility variety known to be susceptible to clubroot;

[0045] The test steps are as follows:

[0046] (1) The identification of mycorrhiza of Plasmodium brassicae, according to the following conventional methods:

[0047] Root swelling caused by clubroot infection only occurs in cruciferous crops. It is mainly distinguished from root swelling caused by root-knot nematodes. The root epidermis enters the cambium, which stimulates the parenchyma cells to divide and expand, and finally causes the root to form a nodule. Root swelling caused by clubroot can occur in both the main and lateral roots of the crop. The nodules on the main root are mostly close to the upper part, round or oval, with uneven and rough surfaces, and may crack or not crack later; the ...

Embodiment 2

[0077] Test materials: Chinese cabbage C10053, Chinese cabbage C10054, Chinese cabbage C10055, Chinese cabbage C10056;

[0078] The control is: Chinese cabbage 83-1, a high-susceptibility variety known to be susceptible to clubroot;

[0079] Test procedure is except that following operational measures are different, and all the other operational measures are identical with embodiment 1:

[0080] (2) Mycorrhizal inoculation method of Plasmodium brassicae in laboratory freezer box

[0081] (1) Preparation of culture substrate

[0082] The perlite is soaked in acidic water with a pH of 5.0 for 96 hours before use, and the culture medium is mixed according to the ratio of perlite and crushed Plasmodium brassica mycorrhizae in a ratio of 1 liter: 15 grams;

[0083] (3) Sowing

[0084] Freezing box 1 is placed in the stainless steel pan;

[0085] (6) Put the stainless steel plate filled with the inoculum solution and the freezing box inside the plate (that is, the freezing box w...

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Abstract

The invention discloses a method for evaluating resistance of brassica plants on club root, which belongs to the brassica plant disease resistance evaluation technical field, and is characterized in that: perlite after being soaked by acid water is mixed with crushed brassica plasmodiophora brassicae fungus root according to a ratio of 1L: 10 to 15g to form culture matrix, the culture matrix is filled inside grids of a frozen box, seeds of a specimen to be evaluated and infected specimen seeds infecting the club root are planted inside the grids of the frozen box, one seed is planted inside each grid, the frozen box is arranged inside a ceramic dish, and 1/2MS culture matrix and the crushed brassica plasmodiophora brassicae fungus root are used for being made into inoculant liquid with pH value of 5.5 and content of pathogenic bacteria spores of 1*108/ml; and the inoculant liquid is filled into the ceramic dish to be cultured inside an artificial weather box, and the resistance of the specimen to be evaluated on the club root is evaluated according to the investigated disease indexes. Due to the adoption of the method, mass evaluation of the resistance on the club root can be realized, a great amount of land can be saved, consistence of the evaluation conditions can be guaranteed, time and labor can be saved, the evaluation result is accurate and reliable, and simplicity and convenience in operation can be realized.

Description

technical field [0001] The invention belongs to the technical field of disease resistance identification of Brassica crops, and in particular relates to an identification method for clubroot resistance. Background technique [0002] Club root is caused by Plasmodium brassicae ( Plasmodiophora brassicae ) is a worldwide disease caused by infecting plants. Brassicina genus Phizodiomycetes Fungi Kingdom Flagellate subphylum Plagidiomycetes Plasmodiophora ), is a pathogenic pathogenic bacteria that specifically infects Brassica plants. The site swells to form a nodular root. On the one hand, the appearance of swollen roots reduces the ability of the root system to absorb and supply water and nutrients, causing the aboveground part to lose water, wither and die; on the other hand, the plant root system loses the protective effect of the outer layer and is easily invaded by other bacteria causing decay. Brassica ( brassica ) is the specific host of Plasmodium, under high t...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/06
Inventor 和江明胡靖锋汪骞钟利杨红丽田志梅
Owner 云南省农业科学院园艺作物研究所
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