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Breeding method of Chinese cabbages resistant to clubroot

A technology against clubroot and clubroot, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and microbial determination/inspection, etc. application and other issues to achieve rapid screening, shorten the breeding cycle, and avoid the spread of germs

Active Publication Date: 2017-03-22
QINGDAO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fine Chinese cabbage varieties mainly planted in my country are generally not resistant to clubroot, and there is a problem that the fine varieties cannot continue to be produced and applied in clubroot disease-affected areas
The improvement of the clubroot resistance traits of the main excellent varieties currently in production is an urgent demand in production, but the transfer and backcross improvement of the clubroot resistance traits are a long process, and it takes a cross breeding, 5-6 Generation backcross selection, 2-3 generations of self-purification, the current technology can achieve up to 2 generations of transfer per year, and the completion of the target material transfer requires 9-10 generations, which takes more than 5 years; and the existing technology has passed The method of inoculation identification of clubroot pathogen to screen resistant individual plants has the risk of spreading the pathogen contamination

Method used

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  • Breeding method of Chinese cabbages resistant to clubroot
  • Breeding method of Chinese cabbages resistant to clubroot
  • Breeding method of Chinese cabbages resistant to clubroot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In mid-April 2014, the target parent Y1 (clubroot resistance genotype rr) and the source CRA-2 (DH line, clubroot resistance genotype RR) were crossed to obtain F1 generation seeds.

[0048] The seed pods of F1 were picked in early May, and the immature embryos were peeled off and placed in a petri dish covered with moist filter paper (see figure 1 ), germinated in a 24°C incubator, and the germinated immature embryos (see figure 2 ) in a refrigerated incubator (SANYO MEDICALCOOL) at 4°C for vernalization under low light (see image 3 ), sowed in June, cultivated in an artificial climate box (see Figure 4 ), the culture temperature is 24°C, the humidity is 69%, and the light is 24 hours. The BC1 generation seeds are obtained by backcross pollination in July, and the BC1 generation seeds are peeled off in August and germinated at 25°C for 6 days.

[0049] The immature embryos of the BC1 generation after germination were vernalized at 5°C for 3 weeks, sown in Septembe...

Embodiment 2

[0057] In mid-April 2014, the target parent Y1 (clubroot resistance genotype rr) and the source CRA-2 (DH line, clubroot resistance genotype RR) were crossed to obtain F1 generation seeds.

[0058] In the first ten days of May, the seed pods of F1 were picked, and the immature embryos were removed and germinated in a 24°C incubator on a petri dish covered with wet filter paper, and the germinated immature embryos were placed in a refrigerated incubator (SANYO MEDICALCOOL) at 4-5°C with low light Cultivate vernalization, sow in June, cultivate in an artificial climate box, culture temperature 24°C, humidity 66%, 24 hours light, backcross pollination in July to obtain BC1 generation seeds, August BC1 generation seeds to peel off embryos, 25°C to accelerate germination 6 sky.

[0059] The immature embryos of the BC1 generation after germination were vernalized at 5°C for 3 weeks, sown in September, and identified by the KBrH129J18R molecular marker in October, from which the BC1 ...

Embodiment 3

[0067] In mid-April 2014, the target parent Y1 (clubroot resistance genotype rr) and the source CRA-2 (DH line, clubroot resistance genotype RR) were crossed to obtain F1 generation seeds.

[0068] In the first ten days of May, the seed pods of F1 were picked, and the immature embryos were stripped and germinated in a culture dish covered with wet filter paper in a 24°C incubator. Planted in June, cultured in an artificial climate box at 24°C, 68% humidity, 24 hours of light, backcross pollination in July to obtain BC1 generation seeds, in August BC1 generation seeds were stripped of embryos and germinated at 25°C for 6 days.

[0069] The immature embryos of the BC1 generation after germination were vernalized at 5°C for 3 weeks, sown in September, and identified by the KBrH129J18R molecular marker in October, from which the BC1 generation resistant single plant with the clubroot resistance gene Rr was screened out. In November, the BC1 generation The disease-resistant single ...

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Abstract

The invention provides a breeding method of Chinese cabbages resistant to clubroot. The breeding method comprises the following steps: 1) a to-be-transferred parent A and a transfer source parent B are hybridized and F1-generaiton seeds are obtained; 2) young embryos of the F1-generation seeds are germinated and sown and cultured after being vernalized; 3) F1-generation plants and the to-be-transferred parent A are subjected to backcrossing, and BC1-generation seeds are obtained; 4) young embryos of the BC1-generation seeds are germinated and sown and cultured after being vernalized; 5) BC1-generation plants are screened by means of a molecular marker, and a BC1-generation disease-resistant single plant is obtained; 6) the BC1 disease-resistant single plant and the to-be-transferred parent A are subjected to backcrossing, and BC2-generation seeds are obtained; 7) the young embryos of the BC2-generation seeds are taken as a treatment object, germination, vernalization, sowing, screening and backcrossing in the step 4), the step 5) and the step 6) are repeated four times, and BC6-generation seeds are obtained; 8) a BC6-generation plant is self-crossed, a BC6-generation self-crossed progeny is obtained, and a transfer single plant with homozygous clubroot resistance is obtained.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for breeding Chinese cabbage resistant to clubroot. Background technique [0002] Cruciferous clubroot is a worldwide disease caused by Plasmodiophora brassica Woronin, which has a wide range of hosts, including cabbage, cabbage, radish, cauliflower, rapeseed, etc. More than 100 species of cultivated and wild cruciferous plants. Chinese cabbage is one of the most serious cruciferous vegetables caused by clubroot. In recent years, clubroot has occurred in most parts of the country, including coastal areas, Yunnan, Guizhou, Sichuan, parts of northeast my country, and western regions, and the area of ​​occurrence has increased sharply. Aggravating year by year, clubroot has become the main disease in the main producing areas of Chinese cabbage. [0003] Because the dormant spores of clubroot have strong stress resistance and can survive in the soil for more than 10 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/68
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13
Inventor 杨晓云张清霞张淑霞司朝光王媛李剑
Owner QINGDAO ACAD OF AGRI SCI
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